PROBLEM TO BE SOLVED: To provide a therapeutic method for a malignant tumor in which a cell-specific expression replication vector is constructed in order to treat the malignant tumor by expressing and replicating a gene in a specific cell, for example, that of the malignant tumor while normal cells may not be damaged, wherein the vector is transferred into the specific living cell, e.g. the malignant tumor cell, and expressed for treatment.
SOLUTION: A transcription initiation control region of human calponin gene specifically expressed in a cell is acquired and linked to the upstream portion of a replication-related gene for a virus, e.g. ICP4. A DNA encoding the protein of vascularization inhibitory factor or apoptosis-relating factors is linked through IRES to the replication-related gene for the virus, while a thymidinekinase gene is left intact. The resultant DNA sequence is incorporated into a virus DNA to construct the cell-specific expression replication vector and the constructed vector is infectively transferred into the malignant tumor cell to selectively break the tumor cells.