PROBLEM TO BE SOLVED: To efficiently and easily carry out the cell culture by culturing a cell in a culture liquid containing lectin, separating the cell coagulated by the action of lectin from the culture liquid, supplying the fresh culture liquid to the separated cell and repeating the cell culture.
SOLUTION: This process is a process for culturing a cell such as hybridoma, etc., producing monoclonal antibody. The cell is suspended in a culture liquid before or after the addition of a lectin [e.g. kidney bean lectin (PHA-L)], the cell is cultured, the cell coagulated by the action of the lectin in the culture liquid is separated from the culture liquid by the gravitational spontaneous precipitation, etc., a part or total of the culture liquid is removed, new culture liquid is added to the cell, the lectin is added to the culture liquid at an arbitrary timing and the culture of the cell is continued. The above procedures are repeated arbitrary times to enable the automation of culture on an industrial scale and the efficient and easy perfusion culture of the objective cell.