２－アセチルアミノ－４－Ｏ－（２－アミノ－２－デオキシ－β－Ｄ －グルコピラノシル）－２－デオキシ－Ｄ －グルコースおよびその塩の製造法
PROBLEM TO BE SOLVED: To obtain the subject compound useful as a substrate for measuring activity of chitin-related compound-decomposing enzymes, a food material and an intermediate in the field of sugar chain engineering, by reacting di-N- acetylchitobiose with a deacetylation enzyme derived from microbes.
SOLUTION: This compound is obtained by reacting di-N-acetyl-D-chitobiose as a substrate, which is easily available as a decomposition product of chitin or a reagent, with a deacetylation enzyme derived from microbes. The compound expressed by the formula or its acid addition salt is almost quantitatively obtained. Chitin-deacetylating enzyme derived from Colletotrichum lindemuthianum ATCC 56676 strain is exemplified as deacetylation enzymes.