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極限ストレス土壌における植物の耐性戦略 アルカリ土壌耐性イネの創製

研究報告コード R000000508
掲載日 2002年9月30日
研究者
  • 森 敏
研究者所属機関
  • 東京大学大学院農学生命科学研究科
研究機関
  • 東京大学大学院農学生命科学研究科
報告名称 極限ストレス土壌における植物の耐性戦略 アルカリ土壌耐性イネの創製
報告概要 不良土壌での作物生産力を高めるため,3つの方針で作物の鉄吸収力を高める研究を進めた。鉄欠乏耐性イネの創製のために遺伝子導入法を用いた。図1に示したように,鉄欠乏で根圏に分泌する「ムギネ酸」の生合成経路を解明し,経路関連酵素を精製,遺伝子クローニングを行った。これらのオオムギの遺伝子3つをイネに導入し検定した結果,naatが耐性を示した(図2)。今後これをアルカリ土壌耐性イネの品種改良の母本とし,さらに高品質のイネと掛け合わせる予定である。陸稲やトウモロコシにも同様の遺伝子を導入して検定を行っている。次に鉄系被覆肥料の開発として,2価鉄塩をポリオレフィンで被覆した粒状肥料と作物種子とを接触施肥する方法を考案し,陸稲の収量を上げることができた。「鉄・ムギネ酸アナログ化合物」を含有する鉄系被覆肥料の開発を目指している。鉄系葉面散布剤としてムギネ酸誘導体を10種類合成し,活性のある3種を見出し,これらの大量合成方法を開発する予定である。Cyanidium caldarium(イデユコゴメ)のアルミニウム耐性のメカニズムについても研究を行った。
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研究分野
  • 土壌化学
  • 土壌管理
  • 肥料の調製,保存
  • 作物の品種改良
  • 稲作
関連発表論文 (1)Bughio N.,Nakanishi H.,Kiyomiya S.,Ishioka N.S.,Watanabe S.,Uchida H.,Tsuji A.,Osa A.,Kume T.,Hashimoto S.,Sekine T.,Mori S.(2001)Real-time[11C]methionine translocation in barley in relation to mugineic acid phytosiderophore biosynthesis.Planta 213(5):708-715
(2)Higuchi K.,Takahashi M.,Nakanishi H.,Kawasaki S.,Nishizawa N.K.,Mori S.(2001)Analysis of toransgenic rice containing barley nicotianamine synthiase gene.Soil Science and Plant Nutrition 47:315-322
(3)Higuchi K.,Tani M.,Nakanishi H.,Yoshiwara T.,Goto F.,Nishizawa N.K.,Mori S.(2001)The expression of a barley HvNAS1 nicotianamine synthase gene promoter-gus fusion gene in transgenic tobacco is induced by Fe-deficiency in roots.Biosci.Biotechnol.Biochem.65(7):1692-1696.
(4)Higuchi K.,Watanabe S.,Takahashi M.,Kawasaki S.,Nakanishi H.,Nishizawa N.K.,Mori S.(2001)Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions.Plant Journal 25:159-168<Abstract>
(5)Kiyomiya S.,Nakanishi H.,Uchida H.,Tsuji A.,Nishiyama S.,Futatsubashi M.,Tsukada H.,Ishioka N.S.,Watanabe S.,Osa A.,Matsuhashi S.,Hashimoto S.,Sekine T.,Mori S.(2001)Real time visualization of 13N translocation in rice under different environmental conditions using prositron emitting tracer imaging system.Plant Physiology 125:1743-1753<Abstract>
(6)Kiyomiya S.,Nakanishi H.,Tsuji A.,Uchida H.,Nishiyama S.,Tsukada H.,Ishioka N.S.,Watanabe S.,Osa A.,Mizuniwa C.,Matsuhashi S.,Hashimoto S.,Sekine T.,Mori S.(2001)Light activates H215O flow in rice - Deatailed monitoring using a positron-emitting tracer imaging syastem(PETIS)Physiologia Plantarum(in press)
(7)Kobayashi T.,Nakanishi H.,Takahashi M.,Kawasaki S.,Nishizawa N.K.,Mori S.(2001)In-vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2'-deoxymugineic acid to mugineic acid in transgenic rice.Planta 212:864-871<Abstact>
(8)Mori S.(2001)The role of mugineic acid in iron acquisition:progress in cloning the genes for transgenic rice.Plant Nutrient(eds.N.Ae,J.Arihara,K.Okada,A.srinivasan)Springer-Verlag Tokyo,pp.120-139
(9)Takahashi M.,Nakanishi H.,Kawasaki S.,Nishizawa N.K.,Mori S.(2001)Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes.Nature biotechnology 19:466-469<Abstact>
(10)Itai R.,Suzuki K.,Yamaguchi H.,Nakanishi H.,Nishizawa N.K.,Yoshimune E.,Mori S.(2000)Induced activity of adnenine phoaphoribosyItransferase(APRT)in iron-deficient barley roots:a possible role for phytosiderophore production.Journal of Experimental Botany 51:1179-1188.<Abstact>
(11)Mori S.,Kiyomiya S.,Nakanishi H.,Ishioka N.S.,Watanabe S.,Osa A.,Matsuhashi S.,Hashimoto S.,Sekine T,Uchida H.,Nishiyama S.,Tsukada H.,Tsuji A.(2000)visalization of 15O-water flow in tomato and rice in the light and dark using a positron-emitting tracer imaging system(PETIS)Soil science and Plant Nutrition 46:975-979.<Abstact>
(12)Nakanishi H.,Yamaguchi H.,Sasakuma T.,Nishizawa N.K.,Mori S.(2000)Two dioxygenase genes,Ids3 and Ids2,from Hordeum vulgare in the biosynthesis of mugineic acid family phytosiderophores.Plant Molecular Biology 44(2);199-207<Abstact>
(13)Singh K.,Mori S.,Yoshimura E.(2000)physiological basis of Al toxicity.disorders and dynamics of tolerance mechanism in acid soils.Advances in Plant Pysiology,vol.3(ed.by hemantaranjan A)scientific publishers(India),Jdohpur,pp.335-352
(14)singh K.,Sasakuma T.,Bughio N.,Takahashi M.,Nakanishi H.,Yoshimune E.,Nishizawa N.K.Mori S(2000)Ability of ancestral wheat species to secrete Mugineic acid family phytosiderophores in response to iron deficiency.Journal of Plant Nutrition 23:1973-1981.<Abstact>
(15)Yamaguchi H.,Nakanishi H.,Nishizawa N.K.,Mori S.(2000)induction of the IDI1 gene in Fe-deficient barley roots:a gene encoding a putative enzyme that catalyses the methionine salvage pathway for phytosiderophore production.Soil Science and Plant Nutrition 46(1):1-9.<Abstact>
(16)Yamaguchi H.,Nakanishi H.,Nishizawa N.K.,and Mori S.(2000)Isolation and characterization of IDI2,a new Fe-deficiency induced cDNA from barley roots,which encodes a protein related to the a subunit of eukaryotic initiation factor 2B(eIF2Ba).Journal of Experimental Botany 51(353):2001-2007.<Abstact>
(17)Yoshimura E.,Kobayashi R.,Furihata K.,Kajiwara H.,Yamazaki S.(2000)1H and 13C NMR spectal assignment of phytochelatin.Magnetic Resonance in chemistry 38:141-142
(18)Yoshimura E.,Sakaguchi T.,Nakanishi H.,Nishizawa N.K..,Nakai I.,Mori S.(2000)Characuterization of the chemical state of iron in the leaves of wild-type tomato and of a nicotianamine-free mutant chloronerva by X-ray absorption near-edge structure(XANES).Phytochemical Analysis 11(3): 160-162.<Abstract>
(19)Yoshimura E.,Sato N.,Nishizawa N.K.,Satake K.,Mori S.(2000)Accumulation of metals in the liverwort.Scapania undulata.Journal of Environmental Science & Health 35(6):837-847.
(20)Yoshimura E.,Nagasaka S.,Satake S.,Mori S.(2000)Mechanism of aluminum tolerance in cyanidium caldarium.Hydrobiologia 433,1005-1010.
(21)Yoshimura E.,Tsunoda K.,Takatsu A.,Satake K.(2000)The determination and speciation of aluminum and its behavior in the environment.Global Environ.Res.4(1),61-71(2000).
(22)Higuchi K.,Nakanishi H.,Suzuki K.,Nishizawa N.K.Mori S.(1999)Presence of nicotianamine synthase isozymes and their homologues in the root of graminaceous plants.Soil Science and Plant Nutrition 45(3):681-691.<Abstract>
(23)Higuchi K.,Suzuki K.,Nakanishi H.,Yamaguchi H.,Nishizawa N.K.,Mori S.(1999)Cloning of nicotianamine synthase genes, nevel genes involved in the biosynthesis of phytosiderophores.Plant Physiology 119(2):471-480.<Abstract>
(24)Kaneko M.,Yoshimura E.,Nishizawa N.K.,Mori S.(1999) Time course study of aluminum-induced callose formation in barley roots as observed by digital microscopy and low-vacuum scanning electron microscopy.Soil Science and Plant Nutrition 45(3):701-712.<Abstract>
(25)Mori S.(1999) Iron acquisition by plants.Current opinion of Plant Biology 2:250-253 <Abstract>
(26)Nakanishi H.,Bughio N.,Matsuhashi S.,Ishioka N.S.,Uchida H.,Tsuji A.,Osa A.,Sekine T.,Kume T.,Mori S.(1999) Visualising real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a positron emitting tracer system(PETIS).Journal of experimental Botany 50(334):637-643.<Abstract>
(27)Oki H.,Yamaguchi H.,Nakanishi H.,Mori S.(1999) Introduction of the reconstructed yeast ferric reductase gene,refrel,into tobacco.Plant and Soil 215(5):211-220.<Abstract>
(28)Sakaguchi T.,Nishizawa N.K.,Nakanishi H.,Yoshimura E.,Mori S.(1999)The role of potassium in the secretion of mugineic acids family phytosiderophores from iron-deficient barley roots.Plant and Soil 215(2):221-227.<Abstract>
(29)Suzuki K.,Higuchi K.,Nakanishi H.,Nishizawa N.K.,Mori S.(1999) Cloning of nicotianamine synthase genes from arabidopsis thaliana.Soil Science and Plant Nutrition 45(4):993-1002.<Abstract>
(30)Takahashi M.,Yamaguchi H.,Nakanishi H.,Shioiri T.,Nishizawa N.K.,Mori S.(1999)Cloning two genes for nicotianamine aminotransferase,a critical enzyme in iron acquisition (Strategy II)in graminaceous plants.Plant Physiologi 121:947-956.<Abstract>
(31)Yoshimura E.,Nagasaka S.,Sato Y.,Satake K.,Mori S.(1999) Extraordinary high aluminium torerance of the acidophilic thermophilic alga,Cyanidium caldarium.Soil Science and Plant Nutrition 45(3):721-724.<Abstract>
(32)Kanazawa K.,Higuchi K.,Nakanishi H.,Nishizawa N.K.,Mori S.(1998)Characterizing nicotianamine aminotransferase:Improving its assay system and details of regulation of its activity by Fe nutrition status.Soil Sciensce and Plant Nutrition 44(4):717-721.
(33)Masaoka Y.,Chino M,Mori S.(1998)Amino acid sequence of proteins indced in Fe-deficient stressed alfalfa(Medicago sativa L.)roots.
(34)Mori S.(1998)Iron transport in graminaceous plants.In Metal Irons in Biological Systems,vol.35,'Iron Transport and Storage in Microorganisms,Plants,and Animals'.Edited by Sigel A.and Sigel H.pp.215-238.Marcel Dekker Inc.,NewYork.
(35)Suzuki K.,Itai R.,Suzuki K.,Nakanishi H.,Nnishizawa N.K.,Yoshimura E.,Mori S.(1998)Formate dehydorogenase,an enaerobic of anaerobic metaboism,is induced by iron deficiency in barley roots.Plant Physiology 116(2):725-732.<Abstract>
(36)Yoshimura E.,Kitai K.,Nishizawa N.K.,Satake K.,Mori S.,Yamazaki S.(1998)Accumulation of metals and cellular distribution of aluminum in the liverwort scapania undulata in scidic and neutral stream in Japan.Journal of Environmental Science &Health A33:671-680.<Abstract.>
(37)Bughio N.,Takahashi M.,Yoshimura E.,Nishizawa N.K.,Mori S.(1997)Light dependent iron transport into isolated barley chloroplasts.Plant and Cell Physiology 38(1):101-105.<Abstract>
(38)Bughio N.,Takahashi M.,Yoshimura E.,Nishizawa N.K.,Mori S.(1997)characteristics of light-regulated iron transport system in barley chloroplasts.Soil Science and Plant Nutrition 43:959-963.<Abstract>
(39)Mori S.(1997)Reevalation of the genes induced bu iron deficiency in barley roots.Soil Science and Plant Nutrition 43:975-980.<Abstract>
(40)Nakanishi H.,Nishizawa N.K.Yoshimura E.,Mori S.(1997)Possible involvement of Ids3 in the hydroxylation of deoxymugineic acid to mugineic acid in Fe-deficient barley roots.In Plant Nutrition-for sustainable food production and environment.Edited by T.Ando et al.pp.281-282.Kluwer Academic Publishers.
(41)Singh K.,Yoshimura E.,Bughio N.,Kanazawa K.,Mori S.(1997)Complexing ability of organic acids with aluminum in acidic soils.In Plant Nutrition-for sustainable food production and environment.Edited by T.Ando et al.pp.447-448.Kluwer Academic Publishers.
(42)Suzuki K.,Kanazawa K.,Higuchi K.,nishizawa N.K.,Mori S.(1997)Immunological characterization of a 36 kDa Fedeficiency specific peptid in barley roots.BioMetals 10(2):77-84.<abstract>
(43)Takahashi M.,Yamaguchi H.,Nakanishi H.,Kanazawa K.,Shioiri T.,Nishizawa N.K.,Mori S.(1997) Purification,characterization and sequencing of nicotianamine aminotransferase(NAAT-III)expressed in Fe-deficient barley roots.In Plant Nutrition-for sustainable food production and environment.Edited by T.Ando et al.pp.279-280.Kluwer Academic Publishers.
(44)Yoshimura E.,Satoh N.,Kaneko M.,Nishizawa N.K.,Satake K.,Mori S.(1997)Cellular distribution and chemical forms of aluminum in Scapania undulata.In Plant Nurrition-for sustainable food production and environment.Edited by T.Ando et al.pp.457-458.Kluwer Academic Publishers.
(45)Higuchi K.,Kanazawa K.,Nishizawa N.K.,Mori S.(1996)The role of nicotianamine synthase in response to Fe nutrition status in Gramineae.Plant and Soil 178(2):171-177.<Abstract>
(46)Higuchi K.,Nishizawa N.,Roemheld V.,Marschner H.,Mori S.(1996)Absence of nicotianamine synthase activity in the tomato mutant 'chloronerva'.Journal of Plant Nutrition 19(8-9):1235-1239.<Abstract>
(47)Huang C.,Graham R.D.,Barker S.J.and Mori S.(1996)Differential expression of iron deficiency-induced genes in barley genotypes with differing manmanese efficiency.Journal of Plant Nutrition 19(2):407-420
(48)Mori S.(1996)Can Modern technology ameliorate desart?In Watanuki K.,Yoshioka K.eds.,Global Environment and Human Activity,Maruzen,Japan
(49)Takizawa R.,Nishizawa N.K.,Nakanishi H.,Mori S.(1996)Effect of iron deficiency on S adenosyl methionine synthetate in barley roots.Journal of Plant Nutrition 19(8-9):1189-1200.<Abstract>
研究制度
  • 戦略的基礎研究推進事業、研究領域「極限環境状態における現象」研究代表者 森 敏(東京大学大学院農学生命科学研究科)/科学技術振興事業団
研究報告資料
  • 森 敏. 極限ストレス土壌における植物の耐性戦略 アルカリ土壌耐性イネの創製. 戦略的基礎研究推進事業「極限環境状態における現象」兼平成8年度採択研究課題終了シンポジウム 講演要旨集,2001. p.42 - 43.

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