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生体光エネルギー変換の分子機構-光化学系Ⅱ複合体の構造と機能の解明と応用

研究報告コード R070000209
整理番号 R070000209
掲載日 2008年4月11日
研究者
  • 沈 建仁
研究者所属機関
  • 岡山大学大学院自然科学研究科
研究機関
  • 岡山大学大学院自然科学研究科
報告名称 生体光エネルギー変換の分子機構-光化学系Ⅱ複合体の構造と機能の解明と応用
報告概要 光合成光化学系II(以下PSIIとする)はラン色細菌から高等植物までのチラコイド膜上に存荘する、14-16種の膜貫通サブユニットと3種の膜表在性サブユニットを含む分子量320kDaの超分子複合体である。この複合体は太陽の光エネルギーを吸収し、一連の電子伝達反応を通して生物利用可能な化学エネルギーに変換し、同時に水を分解し分子状酸素を放出する。従って、PSIIが生物エネルギーの固定と地球上酸素の供給と言う点で極めて重要な複合体である。本研究は、PSII複合体を精製・結晶化し、X線結晶構造解析法を用いてその立体構造を解明することにより、PSIIにおける一連のエネルギー伝達・電子伝達、水分解・酸素発生反応の機構を詳細に解明することを目的とした。
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研究分野
  • 分子構造
  • 光合成
関連発表論文 (1) Kamiya N. and Shen J.-R. (2003) Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7 Å resolution. Proc. Natl. Acad. Sci. USA 100, 98-103.
(2) Koulougliotis D., Shen J.-R., Ioannidis N. and Petrouleas V. (2003) Near-IR irradiation of the S2 state of the water oxidizing complex of photosystem II at liquid helium temperatures produces the metalloradical intermediate attributed to S1YZ Biochemistry, 42, 3045-3053.
(3) Hayashi F., Suzuki H., Iwase R., Uzumaki T., Miyake A., Shen J.-R., Imada K., Furukawa Y., Yonekura K., Namba K. and Ishiura M. (2003) ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC. Genes to Cells 8, 287-296.
(4) Henmi T., Yamasaki H., Sakuma S., Tomokawa Y., Tamura N., Shen J.-R. and Yamamoto Y. (2003) Dymamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts: Evidence from light-induced cross-linking of the proteins in the donor-side photoinhibition. Plant Cell Physiol. 44, 451-456.
(5) Enami I., Iwai M., Akiyama A., Suzuki T., Okumura A., Katoh T., Tada O., Ohta H. and Shen J.-R. (2003) Comparison of binding and functional properties of two extrinsic components, cytochrome c550 and a 12 kDa protein, in cyanobacterial PSII with those in red algal PSII. Plant Cell Physiol. 44, 820-827.
(6) Ohta H., Suzuki T., Ueno M., Okumura A., Yoshihara S., Shen J.-R. and Enami I. (2003) Extrinsic proteins of photosystem II: An intermediate member of the PsbQ protein family in red algal PSII. Eur. J. Biochem. 270, 4156-63.
(7) Tan C.-Y., Xu C.-H., Shen J.-R., Sakuma S., Yamamoto Y., Balny C, and Ruan K.-C. (2003) Thermodynamic and Kinetic Analysis of Unfolding of P23k Protein Isolated from Spinach Photosystem II (in Chinese with English Abstract). Acta Biochimica et Biophysica Sinica, 35, 677-681.
(8) Vasil'ev S., Shen J.-R., Kamiya N. and Bruce D. (2004) The orientations of core antenna chlorophylls in photosystem II are optimized to maximize the quantum yield of photosynthesis. FEBS Lett. 561, 111-116.
(9) Hino T., Kanamori E., Shen J.-R. and Kouyama T. (2004) An Icosahedral assembly of the Light-Harvesting Chlorophyll a/b Protein Complex from Pea Chloroplast Thylakoid Membranes. Acta Crystallogr. D, 60, 803-809.
(10) Weng J., Tan C.-Y., Shen J.-R., Yu Y., Zeng X.-M., Xu C.-H. and Ruan K.-C. (2004) pH-induced conformational changes in the soluble manganese stabilizing protein of photosystem II. Biochemistry, 43, 4855-4861.
(11) Tan C.-Y., Xu C.-H., Wong J., Shen J.-R., Sakuma S., Yamamoto Y., Lange R., Balny C,, and Ruan K.-C. (2005) Pressure Equilibrium and Jump Study on Unfolding of 23kDa Protein from Spinach Photosystem II. Biophys J. 88. 1264-1275.
(12) Arskoeld S.P., Smith P. J., Shen J.-R., Pace R. J. and Krausz E. (2005) Key cofactors of photosystem II cores from four organisms identified by 1.7-K absorption, CD and MCD. Photosynthesis Res. 84, 309-316.
(13) Enami I., Suzuki T., Tada O., Nakada Y., Nakamura K., Tohri A., Ohta H., Inoue I. and Shen J.-R. (2005) Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II. FEBS Journal, 272, 5020-5030.
(14) Kamata T., Hiramoto H., Morita N., Shen J.-R., Mann N. H. and Yamamoto Y. (2005) Quality control of photosystem II: An FtsH protease plays an essential role in the turnover of the reaction center D1 protein in Synechocystis PCC 6803 under heat stress as well as light stress conditions. Photochemical & Pbotobiological Sciences, in press (advance web publication, Sept. 9, 2005).
(15) Yamamoto Y., Sakuma S. and Shen J.-R. (2004) Isolation of photosystem II-enriched membranes and the oxygen-evolving complex (OEC) subunit proteins from higher plants. In Methods in Molecular Biology: Photosynthesis Protocols (ed. by R. Carpentier), 274:29-36. Humana Press.
(16) Shen J.-R. and Kamiya N. (2005) 3D Crystal Structure of the Core-I, In Photosystem II: The Water/Plastoquinone Oxido-Reductase In Photosynthesis, Edited by T. Wydrzynski and K. Satoh, Springer, in press.
(17) 神谷信夫、沈建仁(2005)光化学系II複合体の構造と機能、「蛋白質 核酸 酵素」、Vol. 50, No. 10, 1174-1179.
(18) Shen J.-R. Molecular mechanism of photosystem II based on its crystal structure analysis. Annual Meeting and the 40th Anniversary Meeting of the Founding of the Chinese Society for Plant Physiology, Hangzhou, China (2003.4).
(19) Shen J.-R. and Kamiya N. (Plenary Lecture) Crystal stucture of photosystem II from Thermosynechococcus vulcanus. 11th International Symposium on Phototrophic Prokaryotes, Tokyo (2003.8).
(20) Shen J.-R. and Kamiya N. Crystal structure of Photosystem II from Thermosynechococcus vulcanus. Japan-Swiss Joint Seminar “Biogenesis, Function and Acclimation of the Photosynthetic Apparatus”, Okayama (2003.9).
(21) Shen J.-R, and Kamiya N. Functions of carotenoids and other pigments in photosystem II from a structural point of view. 10th Congress of the European Society for Photobiology, Vienna, Austria (2003.9).
(22) Shen J.-R. Crystal structure of PSII from Thermosynechococcus vulcanus. 314th WE-Heraeus-Seminar: Water Oxidation in Photosynthesis, Bad Honnef. Germany (2003.11).
(23) Shen J.-R,, Naitow H., Kamiya N. (Plenary Lecture) Crystal structure of photosystem II from Thermosynechococcus vulcanus and its functional implications. “Photosynthesis and Post-Genomic Era”, An International Satellite Meeting in conjunction with the 13th International Congress on Photosynthesis, Trios-Rivieres, Quebec, Canada (2004.8).
(24) Shen J.-R. Functional analysis of photosystem II based on its three-dimensional structure. Japanese-Finnish Collaboration Seminar “Molecular Mechanisms of Regulation of Photosynthesis by Environments” and 7th Nordic Photosynthesis Congress. Turku, Finland (2004.11).
(25) 沈建仁、膜タンパク質複合体の結晶化と結晶構造:光化学系II酸素発生複合体、日本学術振興会産学協力研究委員会・回折構造生物 第169委員会、第14回研究会、仙台、2004年9月26-27
研究制度
  • 戦略的創造研究推進事業 さきがけタイプ(旧若手個人研究推進事業を含む)/たんぱく質関連領域
研究報告資料
  • 沈 建仁. 生体光エネルギー変換の分子機構-光化学系Ⅱ複合体の構造と機能の解明と応用. 2005年度“たんぱく質関連領域”合同シンポジウム 要旨集 「生体分子の形と機能」, 2005. p.41 - 44.

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