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生命活動のプログラム 核内因子による遺伝情報発現制御機構の解明

研究報告コード R990004219
掲載日 2001年2月6日
研究者
  • 田村 隆明
研究者所属機関
  • 千葉大学大学院自然科学研究科
研究機関
  • 千葉大学大学院自然科学研究科
報告名称 生命活動のプログラム 核内因子による遺伝情報発現制御機構の解明
報告概要 細胞機能は遺伝子発現の強弱によって制御されている。DNAを基にしたRNAの合成(転写)は遺伝子発現の基本であり、その制御機構の解明は生命活動の本質を理解する上で必須である。1つの細胞の中では、多くの転写が統合性と多様性という、一見相反する機構に従って行われているが、その詳しい分子機構は不明である。本研究では転写制御で普遍的に用いられる因子TBPを材料にしてこの課題に迫ろうとしている。TBPに結合する因子を検索したところ、新規の転写制御因子TIP120が得られた。この因子は多くの遺伝子を活性化する能力を持つ、新しいものであった。さらに研究を進めていく過程で、TBP類似因子の1つであるTLPを世界に先駆けて発見した。TLPはTBP様の機能は持たないが、TBPが担当しないある一群の遺伝子の発現に関わると考えられた。遺伝子発現の統合と多様性に関わる可能性のある因子を発見したが、今後はこれらの材料を基に、遺伝子発現機構の調和と細胞機能の維持という課題をさらに追及する。
研究分野
  • 生物学的機能
  • 酵素一般
  • 遺伝子発現
  • 細胞生理一般
  • 細胞構成体の機能
関連発表論文 (1)Toshihiko Kishimoto, Kenji Kokura, Noriaki Ohkawa, Yasutaka Makino, Mitsuaki Yoshida, Setsuo Hirohashi, Shin-ichiro Niwa, Masami Muramatsu, and Taka-aki Tamura (1998). Enhanced expression of a new class of liver-enriched B-zip transcription factor, HTF, in hepatocellular carcinomas of rats and humans. Cell Growth Different., 9, 337-344.
(2)Yasutaka Makino, Tsuneyo Mimori, Chika Koike, Yumiko Kurokawa, Masato Kanemaki, Satoshi Inoue, Toshihiko Kishimoto, and Taka-aki Tamura (1998). TIP49, homologous to the bacterial DNA helicase RuvB acts as an autoantigen in human. Biochem. Biophys. Res. Commun., 245, 819-823.
(3)Kenji Kokura, Tomoyoshi Nakadai, Toshihiko Kishimoto, Yasutaka Makino, Masami Muramatsu, and Taka-aki Tamura (1998). Gene expression in hepatomas. J. Gastroenterol. Hepatol., 13, 132-141.
(4)Tomoyoshi Nakadai, Toshihiko Kishimoto, Kenji Kokura, Noriaki Ohkawa, Yasutaka Makino, Masami Muramatsu, and Taka-aki Tamura (1998). Cloning of a novel gene, DB83, that encodes a putative membrane protein. DNA Res., 5, 315-317.
(5)Tetsuya Ohbayashi, Yasutaka Makino, and Taka-aki Tamura (1999). Identification of a mouse TBP-like protein (TLP) distantly related to the Drosophila TBP-related factor. Nucl. Acids Res., 27, 750-755.
(6)Tetsuya Ohbayashi, Toshihiko Kishimoto, Yasutaka Makino, Miho Shimada, Tomoyoshi Nakadai, Tsutomu Aoki, Takefumi Kawata, Shin-ichiro Niwa and Taka-aki Tamura (1999). Isolation of cDNA, Chromosome mapping, and expression of the human TBP-like protein (TLP). Biochem. Biophys. Res. Commun., 255, 137-142.
(7)Yumiko Kurokawa, Masato Kanemaki, Yasutaka Makino, and Taka-aki Tamura (1998) A notable example of an evolutionary conserved gene: studies on a putative DNA helicase TIP49. DNA Sequence, 10, 37-42.
(8)Yoshiyuki Konishi, Noriaki Ohkawa, Yasutaka Makino, Hiroaki Okubo, Ryoichiro Kageyama, Teiichi Furuichi, Katsuhiko Mikoshiba, and Taka-aki Tamura (1999). Transcriptional regulation of mouse type 1 inositol 1,4,5-trisphosphate receptor gene by NeuroD-Related Factor. J. Neurochem., 72, 1717-1724.
(9)Noriaki Ohkawa, Yoshiyuki Konishi, Miho Shimada, Yasutaka Makino, Shingo Yoshikawa, Katsuhiko Mikoshiba and Taka-aki Tamura (1999). Activation of the mouse inositol 1,4,5-trisphosphate receptor type 1 promoter by AP-2. Gene, 229, 11-19.
(10)Yasutaka Makino, Masato Kanemaki, Yumiko Kurokowa, Takehiko Koji, and Taka-aki Tamura (1999). A rat RuvB-like protein, TIP49a, is a germ cell-enriched novel DNA helicase. J. Biol. Chem., 274, 15329-15335.
(11)Tomoyoshi Nakadai, Toshihiko Kishimoto, Yaeko Miyazawa, Yasutaka Makino, Takashi Obinata, and Taka-aki Tamura (1999). HP33: hapatocellular carcinoma-enriched 33-kDa protein with similarity to mitochndrial N-acyltransferase but localized in a microtubule-dependent manner at the centrosome. J. Cell Sci., 112, 1353-1364.
(12)Miho Shimada, Yoshiyuki Konishi, Noriyuki Ohkawa, Chiaki Maruyama, Fumio Hanaoka, Yasutaka Makino, and Taka-aki Tamura (1999). Distribution of AP-2 subtypes in the adult mouse brain. Neurosci. Res., 33, 275-280.
(13)Kentaro Kayukawa, Yasutaka Makino, Shingo Yogosawa and Taka-aki Tamura (1999). A serine residue in the N-terminal acidic region of rat RPB6, one of the common subunits of RNA polymerases, is exclusively phosphorylated by casein kinase II in vitro. Gene, 234, 139-147.
(14)Tomoyoshi Nakadai, Nami Okada, Yasutaka Makino and Taka-aki Tamura (1999). Structure of rat gamma-tubulin and its binding to HP33. DNA Res., 6, 207-209.
(15)Miho Shimada, Tetsuya Ohbayashi, Tomoyoshi Nakadai, Yasutaka Makino, Tsutomu Aoi, Takefumi Kawata, Tomohiro Suzuki, Yoichi Matsuda and Taka-aki Tamura (1999). Analysis of the chicken TBP-like protein (tlp) gene: evidence for a striking conservation of vertebrate TLPs and for a close relationship between tlp and tbp genes. Nucl. Acids Res., 27, 3146-3152.
(16)Masato Kanemaki, Yumiko Kurokawa, Toru Matsu-ura, Yasutaka Makino, Abdull Masani, Katsu-ichiro Okazaki, Takashi Morishita, and Taka-aki Tamura (1999). TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase TIP49a. J. Biol. Chem., 274, 22437-22444.
(17)Taka-aki Tamura, Yoshiyuki Konishi, Noriaki Ohkawa, Miho Shimada, Tetsuya Ohbayashi, Tsutomu Aoki, and Yasutaka Makino (1999). Transcriptional regulation of the inositol 1,4,5-trisphosphate receptor type 1 gene in the central nervous system. In Recent Research Development in Neurochemistry, 2, 125-137, Research Signpost Publisher, Trivandrum
(18)Tsutomu Aoki, Nami Okada, Michiko Ishida, Shingo Yogosawa, Yaustaka Makino, and Taka-aki Tamura (1999) TIP120B: a novel TIP120-family protein that is expressed specifically in muscle tissues. Biochem. Biophys. Res. Commun., 261, 911-916.
(19)Tatsushi Yoshida, Yasutaka Makino, and Taka-aki Tamura (1999). Association of the rat heterogeneous nuclear RNA ribonucleoprotein F with TATA-binding protein. FEBS Lett., 457, 251-254
(20)Yasutaka Makino, Tatsushi Yoshida, Shingo Yogosawa, Keniji Tanaka, Masami Muramatsu, and Taka-aki Tamura (1999).
Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120. Genes to Cells, 4, 529-539.
(21)Yasutaka Makino, Shingo Yogosawa, Kentaro Kayukawa, Frederic Coin, Jcan-Marc Egly, Kazuo Yanamoto, Masami Muramatsu, Zhenxin Wang, Robert G. Roeder, and Taka-aki Tamura (1999). TBP-interacting protein120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism. Mol. Cell. Biol., 19, 7951-7960.
(22)Shingo Yogosawa, Kentaro Kayukawa, Takefumi Kawata, Yasutaka Makino, Satoshi Inoue, Akihiko Okuda, Masami Mutratatsu, and Taka-aki Tamura (1999) Induced Expression, Localization and chromosome mapping of the TBP-interacting protein120A. Biochem. Biophys. Res. Commun., 268, 123-128.
(23)Tatsushi Yoshida, Kenji Kokura, Yasutaka Makino, Vincent Ossipow, and Taka-aki Tamura (1999). Heterogeneous nuclear RNA-ribonucleoprotein F binds to DNA via an oligo(dG)-motif and is associated with RNA polymerase II. Genes To Cells, 4, 707-719.
(24)Kenji Kokura, Toshihiko Kishimoto, and Taka-aki Tamura (2000). Identity between rat htf and human xbp-1 gene: demonstration of gene structure, target sequence, and transcription promotion function of HTF. Gene, 241, 297-307.
(25)Tsukasa Oda, Kentaro Kayukawa, Hiroko Hagiwara, Henrik T. Yudate, Yasuiko Masuho, Yasufumi Murakami, Taka-aki Tamura, and Masa-aki Muramatsu (2000). A novel TBP-binding protein ABT1 activates basal transcription and has a yeast homolog being essential for growth. Mol. Cell. Biol., 20, 1407-1418.
(26)Kazuo Yamamoto, Atsuro Koga, Mika Yamamoto, Yu-ichi Nishi, Taka-aki Tamura, Yasuhisa Nogi, and Masami Muramatsu (2000). Identification of a novel 70 kDa protein that binds to the core promoter element and is essential for ribosomal DNA transcription. Nucl. Acids Res., 28, 1199-1205.
(27)Yasutaka Makino, Masato Kanemaki, Atsuro Koga, Kaoru Osano, Toru Matsu-ura, Yumiko Kurokawa, and Taka-aki Tamura (2000). Chromosome mapping and expression of human TIP49 family genes. DNA Sequence, 11, (in press)
(28)Yoshiyuki Konishi, Tsutomu Aoki, Noriaki Ohkawa, Toru Matsu-ura, Katsuhiko Mikoshiba, and Taka-aki Tamura (2000). Identification of the carboxy-terminal activation domain of NeuroD-related factor (NDRF). Nucl. Acids Res., (in press)
(29)Shinsuke Yanagi, Yasutaka Makino, Kazuya Yamano, Naoki Shimbara, and Taka-aki Tamura (2000). Characterization of MSS1 proteasomal ATPase: DNA helicase activity, cell and tissue distribution, and complex formation with the basal transcription factors. Eur. J. Biochem., (Accepted)
研究制度
  • 戦略的基礎研究推進事業、研究領域「生命活動のプログラム」研究代表者 田村 隆明(千葉大学大学院自然科学研究科)/科学技術振興事業団
研究報告資料
  • 田村 隆明. 生命活動のプログラム 核内因子による遺伝情報発現制御機構の解明. 戦略的基礎研究推進事業 平成10年度 研究年報,1999. p.114 - 119.

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