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器官形成の分子機構

研究報告コード R000000120
掲載日 2002年9月30日
研究者
  • 浅島 誠
研究者所属機関
  • 東京大学大学院総合文化研究科
研究機関
  • 東京大学大学院総合文化研究科
報告名称 器官形成の分子機構
報告概要 試験管内での未分化細胞塊(アニマルキャップ)から新しい臓器として膵臓をつくることに成功した。これらのつくられた膵臓は形態学的分化のみならず,インスリンやグルカゴンなどの遺伝子を発現し,また,免疫組織学的にもこれら特異的タンパク質を合成していることが証明された。また新規の遺伝子としては初期発生に働くノーダルのNodal5をクローニング,解析し,これらが他の因子の中で最も初期に発現していることを示した。他の腎臓形式では遺伝子としてXsal-3,アクチビンのタイプIに細胞内で結合するタンパク質(XARIP)についてクローニングし,これらが腎臓形成に関与することを明らかにした。
研究分野
  • 蛋白質・ペプチド一般
  • 免疫反応一般
  • 細胞分裂・増殖
  • 動物の生化学
  • すい臓
関連発表論文 1.cDNA Cloning and Expression of the Xenopus leavis Vitellogenin receptor. Okabayashi, K., Shoji, H., Nakamura, T., Hashimoto, 0., Asashima, M., and Sugino, H. (1996) Biochem. Biophys. Res. Commun., 224, 406-413.
2.Activin treated urodele ectoderm: a model experimental system for cardiogenesis. Ariizumi, T., Komazaki, S., Asashima, M., and G.M. Malacinski. (1996) mt. J. Devel. Biol., 40, 715-718.
3.Occurrence of Immunoreactive Activin/lnhibin β B in Gonadotrophs, Thyrotrophs, and Somatotrophs of the Xenopus Pituitary Uchiyama, H., Komazaki, S., Asashima, M. and Kikuyama, S. (1996) General and Comparative Endocrinology, 102, 1- 10
4.Determination, induction and pattern formation in early amphibian embryos. Tiedemann, H., Asashima, M., Born, J., Grunz, H., Knoehel, W., and Tiedemann, H. (1996) Develop. Growth Differ., 38, 233-246.
5. Sequential Gene Expression during pronephric Tubule Formation in vitro in Xenopus Ectoderm Uochi, T., and Asashima, M. (1996) Develop. Growth Differ., 38 (6), 625-634.
6.Conversion of ectderm into a neural fate by ATH-3, a ventabrate basic helix - loop-helix gene homologous to Drosophila proneural gene atonal. Takabayasi, K., Takahasi, S., Yokota, C., Tsuda, H., Nakanishi, S., Asashima, M., and Kageyama, R. (1997) EMBO Journal 16(2), 384-395.
7.A novel gene encoding a ferredoxin reductase-ralated protein expressed in the neuroectderm in Xenopus neurula. Hatada, S., Kinoshita, M., Sakumoto, H., Nishihara, R., Noda, M. and Asashima, M. (1997) Gene, 194/2, 297-299.
8.Xenopus FK506-binding protein homolog induces a secondary axis in frog embryos, which is inhibited by coexisting BMP-4 signaling. Nishinakamura, R., Matsumoto, Y., Uochi, T., Asashima, M. and Yokota, T. (1997) Biochem. Biophys. Res. Commun.. 239, 585-591.
9.The Na+, K+-ATPase a subunit requires gastrulation in the Xenopus embryo. Uochi, T., Takahashi, S., Ninomiya, H., Fukui, A. and Asashima, M. (1997) Develop. Growth Differ. 39, 571-580.
10. An interferon regulatory factor-related gene (xIRF-6) is expressed in the posterior mesoderm during the early development of the Xenopus laevis. Hatada, S., Kinoshita, M., Takahashi, S., Nishihara, R., Sakumoto, H., Fukui, A., Noda, M. and Asashima, M. (1997) Gene, 203, 183-188.
11. Midkine Counteracts the Activin Signal in Mesoderm Induction and Promotes Neural Formation. Yokota, C., Takahashi, S., Eisaki, A., Asashima, M., S., Akhter, S., Muramatsu, T.and Kadomatsu, K. J. Biochemistry. 123, 339-346 (1998)
12. Cloning and expression pattern of Xenopus prx-1 (Xprx-1) during embryonic development. Takahashi, S., Uochi, T., Kawakami, Y., Nohno, T., Yokota, C., Kinoshita, K.and Asashima, M. Develop. Growth Deffer. 40, 97-104 (1998)
13. Induction of blood cells in Xenopus embryo explants. Miyanaga, Y., Shiurba, R., Nagata, S., Pfeiffer, J., and Asashima, M. (1998) Develop. Genes and Evolution.. 207, 417-426
14. Pattern of gene expression in the core of Spemann's organizer and activin- treated ectoderm in Cynops pyrrhegaster. Yokota, C., Ariizumi, T. and Asashima, M. Develop. Growth Deffer.40, 335-341 (1998)
15. Axil, a member of axin family, interacts with both GSK-3β and β-catenin and inhibits axis formation of Xenopus embryo. Yamamoto, H., Kishida, S. Uochi, T., Ikeda, S., Koyma, S., Asashima, M. and Kikuchi, A. Mol. Cell. Biol. 18, 2867-2875 (1998)
16. XCIRP (Xenopus homolog of cold-inducible RNA binding protein) is expressed transiently in developing pronephros and neural tissue Uochi, T. and Asashima, M. Gene 211/2, 245-250 (1998)
17. Regulation of the Xmyf-5 and XmyoD Expression Pattern during Early Xenopus Development. Takahashi, S., Esumi, B., Nabeshima, Y., and Asashima, M. Zool. Science 15, 231-238, (1998)
18. Activin-treated ectoderm has complete organizing center activity in Cynops embryos. Ninomiya, H., Ariizumi, T., and Asashima, M.
19. Neural induction in embryos Tiedemann, H., Asashima M., Grunz, H., Knochel, W., and Tiedemann, H. Develop. Growth Differ. 40, 363-376 (1998)
20. In vitro Control of Embryonic Axis Formation by Activin A, Concanavalin A and Retinoic Acid in Xenopus lavis Moriya, N., Yokota, C., Ariizumi, T., and Asashima, M. Zool. Science 15, 879-886, (1998)
21. Activin-Treated Urodele Animal Caps:I.Mesoderm and Endoderm Differentiation of Salamander Animal Caps Ariizumi, T., Takano, K., Ninomiya, H., and Asashima, M. Zool. Science 15, 887-892, (1998)
22. Molecular cloning of XNLRR-1, a Xenopus homolog of mouse neuronal leucine-rich repeat protein expressed in the developing Xenopus nervous system Hayata, T., Uochi, T., and Asashima, M. Gene, 221, 159-166 (1998)
23. Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular glandderived epithelial cells in serum-free collagen gel culture Furue, M., Okamoto, T., Hayashi, H., Sato, J. D., Asashima, M., and Saito, S. In Vitro Cellular & Developmental Biology Animal, 35, 131-135 (1998)
24. cDNA Cloning and Distribution of the Xenopus Follistatin-Related Protein Okabayashi, K., Shoji, H., Onuma, Y., Nakamura, T., Nose, K., Sugino, H., and Asashima, M. Biochem. Biophys. Res. Commun.. 254, 42-48 (1999)
25. A Model system for Organ Engineering: Transplantation of in vitro Induced Pronephros Chan, T., Ariizumi, T., and Asashima, M.
26. Blood cell induction in Xenopus animal cap explants: effects of fibroblast growth factor, bone morphogenetic proteins, and activin Miyanaga, Y., Shiurba, R., and Asashima, M. Dev. Genes Evol. 209, 69-76 (1999)
27. Role of Activin and Other Peptide Growth Factors in Body Patterning in the Early Amphibian Embryo Asashima, M., Kinoshita, K., Ariizumi, T., and Malacinski, G. M. mt. Rev. Cytology 191, 1-52 (1999)
28. Cytochalasin B inhibits morphogenetic movement and muscle differentiation of activin-treated ectoderm in Xenopus Tamai, K., Yokota, C., Ariizumi, T., and Asashima, M. Develop. Growth Differ. 41, 41-49(1999)
29. Peptide growth factors in amphibian embryogenesis: intersection of modern molecular approaches with traditional inductive interaction paradigms Asashima, M., Yokota, C., Takahashi, S., Lau, C. L. and Malacinski, G. M. mt. J. Dev. Biol. 43, 1-10 (1999) 30. Changes in the adhesive properties of dissociated and reaggregated Xenopus laevis embryo cells Kuroda, H., Sakumoto, H., Kinoshita, K. and Asashima, M. Develop. Growth Differ. 41, 283-291 (1999)
31. Activin-treated Urodele Animal Caps: II. Inductive Interactions in Newt Animal Caps After Treatment with Activin A Ariizumi, T., Komazaki, S. and Asashima, M. Zoological Science, 16(1), 115-124 (1999)
32. Endoderm differentiation and inductive effect of activin-treated ectoderm in Xenopus Ninomiya, H., Takahashi, S., Tanegashima, K., Yokota, C. and Asashima, M. Develop. Growth Differ. 41, 391-400 (1999)
33. Activin Incorporation into Vitellogenic Oocytes of Xenopus Laevis Fukui, A., Shiurba, R., Asashima, M. Cellular and Molecular Biology 45(5), 545-554 (1999)
34. Expression of Brachyury-like T-box transcription factor, Xbra3 in Xeopus embryo Hayata, T., Eisaki, A., Kuroda, H., Asashima, M. Dev. Genes Evol. 209, 560-563 (1999)
35. Expression of Xenopus T-box transcription factor, Tbx2 in Xenopus embryo Hayata, T., Kuroda, H., Eisaki, A. and Asashima, M. Dev. Genes Evol. 209, 625-628 (1999)
36. Bioassays of Inductive Interactions in Amphibian Development Ariizumi, T., Takano, K., Asashima, M and Malacinski, G.M. Methods in Molecular Biology 135: Developmental Biology Protocols 1, 89- 112 (1999)
37. Molecular Cloning of a Novel Xenopus spalt Gene (Xsal 3) Onuma, Y., Nishinakamura, R., Takahashi, S., Yokota, T. and Asashima, M. Biochemical and Biophysical Research Communications 264, 151-156 (1999)
38 In vitro control of organogenesis and fundamental embryonic form by the peptide growth factor activin Ariizumi, T., Asashima, M. J. Artif Organs 2, 117-123 (1999)
39. Activation of Stat3 by cytokine receptor gp 130 ventralizes Xenopus embryos independently of BMP-4 Nishinakamura, R., Matsumoto, Y., Matsuda, T., Heike, T., Asashima, M. and Yokota, T. Dev. Biol. 216, 48 1-490 (1999)
40. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis Asashima, M., Ariizumi, T., Takahashi, S. and Malacinski, G.M. Methods in Molecular Biology 136: Developmental Biology Protocols II 15- 26( 1999)
41. Effect of TGF-β on morphogenesis in rat salivary gland-derived RSMG- 1 Cells Furue, M., Okamoto, T. and Asashima, M. Tiss. Cult. Res. Commun. 18:339-343 (1999)
42. Cloning a novel developmental regulating gene, Xotx5 : its potential role in anterior formation in Xenopus laevis Kuroda, H., Hayata, T., Eisake, A. and Asashima M. Develop. Growth Differ. 42, 87-93 (2000)
43. In vitro organogenesis of pancreas in Xenopus laevis dorsal lips treated with retinoic acid Moriya, N., Komazaki, S. and Asashima, M. Develop. Growth Differ. 42, 175-185 (2000)
44. FGF Plays a Critical Role in SM22 α Expression during Xenopus Embryogenesis Oka, T., Shiojima, I., Monzen, K., Kudoh, S., Hiroi, Y., Shiokawa, K., Asashima, M., Nagai, R., Yazaki, Y. and Komuro, I. Arteriosclerosis, Thrombosis, and Vascular biology, 907-914 (2000)
45. Gene expression pattern Cloing and expression pattern of a Xenopus pronephros-specific gene, XSMP-30 Sato, A., Asashima, M., Yokota, T. and Nishinakamura, R. Mech. Dev.92, 273-275 (2000)
46. In vitro control of organogenesis and body patterning by activin during early amphibian development Asashima, M., Ariizumi, T. and Malacinski, G.M. Comparative Biochemistry and Physiology 126, 169-178 (2000)
47. Development of the embryonic kidney Chan, Te-Chuan and Asashima, M. Clin. Exp. Nephrol, 4, 1-10 (2000)
48. Expression of the Xenopus GTP-binding protein gene Ran during embryogenesis Onuma, Y., Nishihara, R., Takahashi, S., Tanegashima, K., Fukui, A. and Asashima, M. Development Genes and Evolution 210, 325-327 (2000)
49. XSIP 1, a Member of Two-Handed Zinc Finger Proteins, Induced Anterior Neural Markers in Xenopus laevis Animal Cap Eisaki, A., Kuroda, H., Fukui, A. and Asashima, M. Biochemical and Biophysical Res. Coin. 271, 151-157 (2000)
50. The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family Tanaka, K., Okabayashi, K., Asashima, M. Perrimon, N. and Kadowaki, T. Eur. J. Biochem. 267, 1-13 (2000)
51. Effect of rat Axin domains on axis formation in Xenopus embryos Fukui, A., Kishida, S. Kikuchi, A. and Asashima, M. Develop. Growth & Differ. 42(5) , 489-498 (2000)
52. In vitro pancreas formation from Xenopus ectoderm treated with activin and retinoic acid Moriya, N., Komazaki, S., Takahashi, S., Yokota, C. and Asashima, M. Develop. Growth & Differ. 42(6), 593-602 (2000)
53. Inhibition of Wnt Signaling Pathway by A Novel Axin-binding Protein Kadoya, T., Kishida, S., Fukui, A., Hinoi, T., Michiue, T., Asashima, M. and Kikuchi, A. J. Biol. Chem. 275(47), 37030-37037
54. Expression cloning of Xantivin, a Xenopus lefty/antivin related gene, involved in the regulation of activin signaling during mesoderm induction Tanegashima, K., Yokota, C., Takahashi, S. and Asashima, M. Mech. Develop. 99, 3-14 (2000)
55. Isoleucine Prevents rat salivary gland epithelial cells from Apoptosis in Serum- Free Culture M. Fume, T. Okamoto, S. Koshika and M. Asashima In Vitro Cell. Dev. Biol. -Animal 36:287-289, May 2000, 2000 Society for In Vitro Biology
56. A Novel β‐Catenin Binding Protein Inhibits β‐Catenin-dependent Tcf Activation and Axis Formation Sakamoto, I., Kishida, S., Fukui, A., Kishida, M., Yamamoto, H., Hino, S., Michiue, T., Takada, S., Asashima, M. and Kikuchi, A. J. Biol. Chem. 275(42), 32871-32878, 2000
57. Meiotic maturation induces animal-vegetal asymmetric distribution of aPKC and ASIP/PAR-3 in Xenopus oocytes M. Nakaya, A. Fukui, Y. Izumi, K. Akimoto, M. Asashima and S. Ohno Development 127, 5021-5031, 2000
58. Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center S. Takahashi, C. Yokota, K. Takano, K. Tanegashima, Y. Onuma, J. Goto and M. Asashima
59. Development 127, 5319-5329 (2000) Effects of rat Axin domains on axis formation in Xenopus embryos A. Fukui, S. Kishida, A. Kikuchi and M. Asashima Develop. Growth Differ. 42,489-498, 2000
60. A Role for Xlim- 1 in Pronephros Development in Xenopus laevis Te-chuan Chan, S. Takahashi and M. Asashima Develop. Biol. 228, 256-269, 2000
61. Inhibition of the Wnt Signaling Pathway by Idax, A Novel Dvl-binding Protein S. Hino, S. Kishida, T. Michiue, A. Fukui, I. Sakamoto, S. Takada, M. Asashima, A. Kikuchi Mol. Cell Biol., 330-342, 2000
62. Dome Formation and Tubule Morphogenesis by Xenopus Kidney A6 Cell Cultures Exposed to Microgravity Simulated with A 3D-Clinostat and to Hypergravity. Junji Ichigi and Makoto Asashima In Vitro Cellular and Developmental Biology - Animal. 37. 31-44, 2001
63. Spemann's influence on Japanese Developmental Biology. Makoto Asashima and Tokindo S. Okada J. Dev. Biol. 45, 273-279, 2001
64. In vitro induction systems for analyses of amphibian organogenesis and body patternig. Takashi Ariizumi and Makoto Asashima .J. Dev. Biol. 45, 273-279, 2001
65. Activin A Induces Expression of Rat Sel-1/mRNA, a Negative Regulator of Notch Signaling, in Rat Salivary Gland-Derived Epithelial Cell. Miho Fume, Yan Zhang, Tetsuji Okamoto, Ryu-Ichiro Hata, and Makoto Asashima Bioch. and Biophy. Res. Comm. 282, 745-749, 2001
66. Inhibition of the Signaling Pathway by the PR61 Subunit of Protein Phosphatase 2A. Hideki Yamamoto, Toshihide Hinoi, Tatsuo Michiue, Akimasa Fukui, Hirofumi Usui, Veerle Janssens, Christine Van Hoof, Jozef Goris, Makoto Asashima, and Akira Kikuchi J. Biol. Chem. 276, 29, 26875-26882, 2001.
67. Inhibition of the Wnt Signaling Pathway by Idax, a Novel Dvl-Binding Protein. Shin-ichiro Hino, Shosei Kishida, Tatsuo Michiuc, Akimasa Fukui, Ikuo Sakamoto, Shinji Takada, Makoto Asashima, and Akira Kikuchi Mol. Cell. Biol. 21, 330-342, 2001 68. Murine homolog of SALL1 is essential for ureteric bud invasion in kidney development. Ryuichi Nishinakamura, Yuko Matsumoto, Kazuki Nakao, Kenji Nakamura, Akira Sato, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Sheila Scully, David L. Lacey, Motoya Katsuki, Makoto Asashima and Takashi Yokota Development 128, 3105-3115, 2001
69. Synergistic Activation of the Wnt Signaling Pathway by Dvl and Casein Kinase Iε. Michiko Kishida, Shin-ichiro Hino, Tatsuo Michiue, Hideki Yamamoto, Shosei Kishida, Akimasa Fukui, Makoto Asashima, and Akira Kikuchi J. Biol. Chem. 276 (35) 33147-33155, 2001
70. Pluripotent cells (stem cells) and their determination and differentiation in early vertebrate embryogenesis. H. Tiedemann, M. Asashima, H. Grunz and W. Knochel Develop. Growth Differ. 43, 469-502, 2001
研究制度
  • 戦略的基礎研究推進事業、研究領域「生命活動のプログラム」研究代表者 浅島 誠(東京大学大学院総合文化研究科)/科学技術振興事業団
研究報告資料
  • 浅島 誠. 生命活動のプログラム 器官形成の分子機構. 戦略的基礎研究推進事業 平成11年度 研究年報.科学技術振興事業団, 2000. p.63 - 67.

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