TOP > 研究報告検索 > 深度地下極限環境微生物の探索と利用

深度地下極限環境微生物の探索と利用

研究報告コード R000000209
掲載日 2002年9月30日
研究者
  • 今中 忠行
研究者所属機関
  • 京都大学大学院工学研究科
研究機関
  • 京都大学大学院工学研究科
報告名称 深度地下極限環境微生物の探索と利用
報告概要 本研究は,深度地下極限環境から分離した微生物が有するであろう特殊酵素,代謝系や環境適応戦略を解明していくことを目標にしている。Thermococcus kodakaraensis KOD1 株由来の超耐熱性酵素の同定と解析研究では,超好熱始原菌T. kodakaraensis KOD1 のDNA リガーゼ遺伝子をゲノム解析により取得し,その一次構造を明らかにした。 KOD1 株のゲノム解析からLon proteaseと相同性の遺伝子断片が得られたので,これまでに研究されていない始原菌内でのLon proteaseの生理的役割の解明研究を進めた。T. kodakaraensis KOD1 株由来のリブロース1,5-二りん酸カルボキシラーゼ/オキシゲナーゼ (Rubisco)が高温下でも十量体構造を維持し,また,温度に依らず安定した立体構造を保持することを明らかにした。ポリアミンはヒストンと相互作用し,高温でのヌクレオソーム構造の維持に関与していることが示唆された。トリプトファンシンターゼの組換え体の調製等も含めて研究の概要を報告した。
研究分野
  • 遺伝子の構造と化学
  • 微生物の生態
  • 菌体の生産
関連発表論文 (1)A unique DNase activity shares the active site with ATPase activity of RecA/Rad51 homologue (Pk-REC) from a hyperthermophilic archaeon. Naeem Rashid, Masaaki Morikawa, Shigenori Kanaya, Haruyuki Atomi & Tadayuki Imanaka FEBS Lett., 445, 111-114, 1999.
(2)In vitro heat effect on functional and conformationa changes of cyclodextrin glucanotransferase from hyperthermophilic archaea. Tomoko Yamamoto, Kentaro Shiraki, Shinsuke Fujiwara, Masahiro Takagi, Kiichi Fukui & Tadayuki Imanaka Biochem. Biophys. Res. Commun., 265, 57-61, 1999.
(3)Identification of the gene encoding esterase, a homolog of hormone-sensitive lipase, from an oil-degrading bacterium, strain HD-1. Satoru Mizuguchi, Kei Amada, Mitsuru Haruki, Tadayuki Imanaka, Masaaki Morikawa & Shigenori Kanaya J. Biochem., 126, 731-737, 1999.
(4)Pk-cdcA, encodes a CDC48/VCP homologue in the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1: transcriptional and enzymatic characterization. S.J. Jeon, Shinsuke Fujiwara, Masahiro Takagi & Tadayuki Imanaka Mol. Gen. Genet., 262, 559-567, 1999.
(5)Hyperthermostable protein structure maintained by intra and inter-helix ion-pairs in archaeal O6-methylguanine-DNA methyltransferase. Hiroshi Hashimoto, Tsuyoshi Inoue, Motomu Nishioka, Shinsuke Fujiwara, Masahiro Takagi, Tadayuki Imanaka & Yasushi Kai J. Mol. Biol., 292, 707-716, 1999.
(6)Characterization and application to hot start PCR of neutralizing monoclonal antibodies against KOD DNA polymerase. Hiroshi Mizuguchi, Miki Nakatsuji, Shinsuke Fujiwara, Masahiro Takagi & Tadayuki Imanaka J. Biochem., 126, 762-768, 1999.
(7)Gene analysis and enzymatic properties of thermostable β-glycosidase from Pyrococcus kodakaraensis KOD1. Satoshi Ezaki, Kohei Miyaoku, Ken-ichi Nishi, Takeshi Tanaka, Shinsuke Fujiwara, Masahiro Takagi, Haruyuki Atomic & Tadayuki Imanaka J. Biosci. Bioeng., 88, 130-135, 1999.
(8)Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam. Nguyen Q. Huy, Sha Jin, Kei Amada, Mitsuru Haruki, Nguyen B. Huu, Dinh T. Hang, Dang T.C. Ha, Tadayuki Imanaka, Masaaki Morikawa & Shigenori Kanaya J. Biosci. Bioeng., 88, 100-102, 1999.
(9)Gene cloning and characterization of aldehyde dehydrogenase from a petroleum-degrading bacterium strain HD-1. Naoko Okibe, Kei Amada, Shin-ichi Hirano, Mitsuru Haruki, Tadayuki Imanaka, Masaaki Morikawa & Shigenori Kanaya J. Biosci. Bioeng., 88, 7-11, 1999.
(10)Sequence and transcriptional studies of five clustered flagellin genes from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. Keisuke Nagahisa, Satoshi Ezaki, Shinsuke Fujiwara, Tadayuki Imanaka & Masahiro Takagi FEMS Microbiol. Lett., 178, 183-190, 1999.
(11)Isolation and characterization of psychrotrophs from subterranean environments. Naeem Rashid, Hiroshi Kikuchi, Satoshi Ezaki, Haruyuki Atomi & Tadayuki Imanaka J. Biosci. Bioeng., 87, 746-751, 1999.
(12)Crystallographic studies on family B DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis strain KOD1. Hiroshi Hashimoto, Tomoya Matsumoto, Motomu Nishioka, Toru Yuasa, Shoko Takeuchi, Tsuyoshi Inoue, Shinsuke Fujiwara, Masahiro Takagi, Tadayuki Imanaka & Y. Kai J. Biochem., 125, 983-986, 1999.
(13)The concept of the α-amylase family; structural similarity and common catalytic mechanism. Takashi Kuriki & Tadayuki Imanaka J. Biosci. Bioeng., 87, 557-565, 1999.
(14)Analysis of DNA compaction profile and intracellular contents of archaeal histones from Pyrococcus kodakaraensis KOD1. Hiroki Higashibata, Shinsuke Fujiwara, Masahiro Takagi & Tadayuki Imanaka Biochem. Biophys. Res. Commun., 258, 416-424, 1999.
(15)Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon, Themococcus sp. Yoshihisa Tachibana, Akiko Kuramura, Naoki Shirasaka, Yuji Suzuki, Tomoko Yamamoto, Shinsuke Fujiwara, Masahiro Takagi & Tadayuki Imanaka Appl. Environ. Microbiol., 65, 1991-1997, 1999.
(16)Isolation of TBP-interacting protein(TIP) from a hyperthermophilic archaeon that inhibits the binding of TBP to TATA-DNA. Tomoki Matsuda, Masaaki Morikawa, Mitsuru Haruki, Hiroki Higashibata, Tadayuki Imanaka & Shigenori Kanaya FEBS Lett., 457, 38-42, 1999.
(17)Isolation and characterization of a second subunit of molecular chaperonin from Pyrococcus kodakaraensis KOD1: Analysis of ATPase deficient mutant. Michi Izumi, Shinsuke Fujiwara, Masahiro Takagi, Shigenori Kanaya & Tadayuki Imanaka Appl. Environ. Microbiol., 65, 1801-1805, 1999.
(18)The expression of tryptophan biosynthesis gene cluster trpCDEGFBA from Pyrococcus kodakaraensis KOD1 is regulated at the transcriptional level as a single mRNA. Xiaofeng Tang, Satoshi Ezaki, Shinsuke Fujiwara, Masahiro Takagi, Haruyuki Atomi & Tadayuki Imanaka Mol. Gen. Genet., 262, 815-821, 1999.
(19)Ribulose bisphosphate carboxylase/oxygenase from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 is composed solely of large subunits and forms a pentagonal structure. Norihiro Maeda, Ken Kitano, Toshiaki Fukui, Satoshi Ezaki, Haruyuki Atomi, Kunio Miki & Tadayuki Imanaka J. Mol. Biol., 293, 57-66, 1999.
(20)A unique chitinase with dual active sites and triple substrate binding sites from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. Takeshi Tanaka, Shinsuke Fujiwara, Shingo Nishikori, Toshiaki Fukui, Masahiro Takagi & Tadayuki Imanaka Appl. Environ. Microbiol., 65, 5338-5344, 1999.
研究制度
  • 戦略的基礎研究推進事業、研究領域「極限環境状態における現象」研究代表者 今中 忠行(京都大学大学院工学研究科)/科学技術振興事業団
研究報告資料
  • 今中 忠行. 極限環境状態における現象 深度地下極限環境微生物の探索と利用. 戦略的基礎研究推進事業 平成11年度 研究年報.科学技術振興事業団, 2000. p.564 - 573.

PAGE TOP