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組換えを介したゲノム動態制御

研究報告コード R000000240
掲載日 2002年9月30日
研究者
  • 柴田 武彦
研究者所属機関
  • 理化学研究所
研究機関
  • 理化学研究所
報告名称 組換えを介したゲノム動態制御
報告概要 本研究では,ゲノムを変化させる一方,逆に変異を取り除く組替えを介したゲノム動態制御について,遺伝子・分子機能から染色体・細胞の挙動までの総合的な理解と高等動物での普遍性の検証,新技術の基盤構築を目指す。以下の課題について研究する,
1)DNA鎖切断導入・修復,ゲノム流動化制御因子の研究,
2)染色体レベルでのゲノム流動性・恒常性制御の研究,
3)動物細胞株でのゲノム改変技術の研究。
平成11年度には,次の分野で成果を得た。
1)ゲノム動態に働く遺伝子,蛋白質,
2)組換え制御に働く染色体構造動態,
3)組換えとその制御に働く分子機構,
4)高等動物細胞細胞ゲノム動態に働く組換え機能。
研究分野
  • 分子遺伝学一般
  • 遺伝子操作
関連発表論文 (1)Furuse, M., Nagase, Y., Tsubouchi, H., Murakami-Murofushi, K., Shibata, T. and Ohta, K. (1998) "Distinct roles of two separable in vitro activities of yeast Mre11 in mitotic and meiotic recombination." EMBO J., 17, 6412-6425.
(2)Ohta, K., Wu, T.-C., Lichten, M. and Shibata, T. (1999) Competitive inactivation of a double-strand DNA break site involves parallel suppression of meiosis-induced changes in chromatin configuration. Nucleic Acids Res., 27, 2175-2180.
(3)Aihara, H., Ito, Y., Kurumizaka, H., Yokoyama, S. and Shibata, T. (1999) "The N-terminal domain of the human Rad51 protein binds DNA: Structure and a DNA binding surface as revealed by NMR." J. Mol. Biol., 290, 495-504.
(4)Kurumizaka, H., Aihara, H., Kagawa, W., Shibata, T. and Yokoyama, S. (1999) "Human Rad51 amino acid residues required for Rad52 binding." J. Mol. Biol., 291, 537-548.
(5)Kurumizaka, H., Ikawa, S., Sarai, A., Shibata, T. (1999) "The mutant RecA proteins, RecAR243Q and RecAK245N, that exhibit defective DNA binding in homologous pairing." Arch. Biochem. Biophys., 365, 83-91.
(6)Rajesh, S., Sakamoto, T., Iwamoto-Sugai, M., Shibata, T., Kohno, T., Ito, Y. (1999) "Ubiquitin binding interface mapping on yeast ubiquitin hydrolase by NMR chemical shift perturbation." Biochemistry, 38, 9242-9253
(7)Senbongi, H., Ling, F., Shibata, T. (1999) "A mutation in a mitochondrial ABC transporter results in mitochondrial dysfunction through oxidative damage of mitochondrial DNA." Mol. Gen. Genet., 262, 436-436
(8)Yamada, T., Okuhara. K., Iwamatsu, A., Seo, H., Ohta, K., Shibata, T. and Murofushi, H. (2000) "p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication," FEBS Lett., 466, 287-291.
(9)Jeong, S.-M., Kawasaki, K., Juni, N. and Shibata, T. (2000) "Identification of Drosophila melanogaster RECQE as a member of a new family of RecQ homologues preferentially expressed in early embryos," Mol. Gen. Genet., 263, 183-193.
(10)Kurumizaka, H., Aihara, H., Ikawa, S. and Shibata, T. (2000). "Specific defects in double-stranded DNA unwinding and homologous pairing of a mutant RecA protein," FEBS Lett., 477, 129-134.
(11)Fox, M. E., Yamada, T., Ohta, K. and Smith, G. R. (2000) "A Family of CRE-related DNA sequences with meiotic recombination hotspot activity in Schizosaccharomyces pombe," Genetics, 156, 59-68.
(12)Miyajima, A., Seki, M., Onoda, F., Shiratori, M., Odagiri, N., Ohta, K., Kikuchi, Y., Ohno, Y. and Enomoto, T. (2000) "Sgsl helicase activity is required for mitotic but apparently not for meiotic functions," Mol. Cell. Biol., 20, 6399-6409.
(13)Ling, F., Morioka, H., Ohtsuka. E. and Shibata, T. (2000). "A role for MHR1, a gene required for mitochondrial genetic recombination, in the repair of damage spontaneously introduced in yeast mtDNA," Nucleic Acids Res., 28, 4956-4963.
(14)Smith, K, N., Penkner, A., Ohta, K., Klein, F. and Nicolas, A. (2001) "B-type cyclins CLB5 and CLB6 control the initiation of recombination and synaptonemal complex formation in yeast meiosis," Current Biology 11, 88-97.
(15)Kurumizaka, H., Ikawa, S., Nakada, M., Eda, K., Kagawa, W., Takata, M., Takeda, S., Yokoyama, S. and Shibata, T. (2001) "Homologous-pairig activity of the human DNA-repair proteins Xrcc3 ・ Rad51C." Proc. Natl. Acad. Sci. USA., 98, 5538-5543
(16)Shibata, T., Nishinaka, T., Mikawa, T., Aihara, H., Kurumizaka, H., Yokoyama, S. and Ito, Y. (2001) "Homolorous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: A possible advantage of DNA over RNA as genomic material." Proc. Natl. Acad. Sci. USA., 98, 8425-8432.
(17)Kagawa, W., Kurumizaka, H., Ikawa, S., Yokoyama, S. and Shibata, T. (2001) "Homologous pairing promoted by the human Rad52 protein." J Biol Chem, 276, 35201-35208.
(18)西中太郎,柴田武彦(1999)"DNAはどのようにして相同部位を認識し,鎖を交換するか?-相同探索,鎖交換反応のDNA分子構造模型-,"蛋白質核酸酵素,44,631-642.
研究制度
  • 戦略的基礎研究推進事業、研究領域「ゲノムの構造と機能」研究代表者 柴田 武彦(理化学研究所)/科学技術振興事業団
研究報告資料
  • 柴田 武彦. ゲノム構造と機能 組換えを介したゲノム動態制御. 戦略的基礎研究推進事業 平成11年度 研究年報.科学技術振興事業団, 2000. p.731 - 744.

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