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PRODUCTION OF HYBRIDOMA PRODUCING ANTIHUMAN CENP-A PEPTIDE MONOCLONAL ANTIBODY AND METHOD OF USING THE SAME 実績あり

Foreign code F110004240
File No. A021-08CA
Posted date 2011年7月13日
Country カナダ
Application number 2474880
Gazette No. 2474880
Gazette No. 2474880
Date of filing 平成14年7月4日(2002.7.4)
Gazette Date 平成15年8月7日(2003.8.7)
Gazette Date 平成21年11月24日(2009.11.24)
Priority data
  • 特願2002-023587 (2002.1.31) JP
Title PRODUCTION OF HYBRIDOMA PRODUCING ANTIHUMAN CENP-A PEPTIDE MONOCLONAL ANTIBODY AND METHOD OF USING THE SAME 実績あり
Abstract

It is intended to provide antibodies, in particular, monoclonal antibodies against, as antigens, peptides comprising N-terminal 1/3 amino acid sequences of a protein CENP-A expressed in centromeres in chromosome and a process for producing th e same. Antibodies against partial-length peptides of the CENP-A protein expressed in centromeres in chromosome (preferably a peptide having the amino acid sequen ce of 3- to 19-positions of the CENP-A protein, a peptide having the amino acid sequence of 27- to 40-positions thereof or a peptide having the amino acid sequence of 1 26- to 140-positions thereof)

and a process for producing the same. A method of solubilizing a centromere chromatin while sustaining its completeness by treating with 0.3 M NaCl and MNase.

A method of using an anti-CENP-A antibody which comprises chemically bonding the antibody to a support and isolating and purifying the same by the immunoprecipitation method.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • OKAZAKI, TSUNEKO,
  • YODA, KINYA,
  • NOZAKI, NAOHITO
IPC(International Patent Classification)
Reference ( R and D project ) CREST Genetic Programming AREA
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