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PRODUCTION METHOD AND KIT OF INDUCED PLURIPOTENT STEM CELLS

Foreign code F210010283
File No. (S2019-0335-N0)
Posted date 2021年1月28日
Country 世界知的所有権機関(WIPO)
International application number 2020JP016924
International publication number WO 2020213725
Date of international filing 令和2年4月17日(2020.4.17)
Date of international publication 令和2年10月22日(2020.10.22)
Priority data
  • 特願2019-078907 (2019.4.17) JP
Title PRODUCTION METHOD AND KIT OF INDUCED PLURIPOTENT STEM CELLS
Abstract This method of producing induced pluripotent stem cells involves a step for introducing an initialization factor into somatic cells of a mammal, and culturing in a neural stem cell culture medium to obtain induced neural stem cell-like cells, and a step for cultivating said induced neural stem cell-like cells in a growth medium to obtain induced pluripotent stem cells , wherein the initialization factor contains an OCT family, a SOX family, a KLF family, a MYC family, a LIN28 family and a P53 function inhibitor.
Outline of related art and contending technology BACKGROUND ART
Techniques for establishing iPS cells from human and mouse somatic cells have been improved and simplified, and the efficiency of establishing iPS cells has been improved. On the other hand, there is an animal (pluripotent induction resistant animal) which is difficult to establish iPS cells from adult somatic cells. Dogs, pigs, marmosets, cats, cows, horses, and the like are known as pluripotent induction resistant animals.
See), for example, (, Non-Patent Document 1, in which canine iPS cells can be established by persistently expressing a reprogramming factor in canine fetus fibroblasts. In addition, see), for example, Non-Patent Document 2, it is possible to establish canine iPS cells from canine fetus fibroblasts by using Sendai virus. See), for example, Non-Patent Document 3, in which porcine iPS cells can be established by persistently expressing a reprogramming factor in porcine fetus fibroblasts.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • KEIO UNIVERSITY
  • NIHON UNIVERSITY
  • Inventor
  • OKANO Hideyuki
  • SHIOZAWA Seiji
  • YOSHIMATSU Sho
  • EDAMURA Kazuya
  • IGUCHI Aozora
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN WS ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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