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Oligonucleotide derivative, labeling agent and use for labeling agent

Foreign code F120006738
File No. K02704WO
Posted date 2012年5月30日
Country アメリカ合衆国
Application number 200913126320
Gazette No. 20120126175
Gazette No. 8354515
Date of filing 平成21年11月2日(2009.11.2)
Gazette Date 平成24年5月24日(2012.5.24)
Gazette Date 平成25年1月15日(2013.1.15)
International application number JP2009068785
International publication number WO2010055789
Date of international filing 平成21年11月2日(2009.11.2)
Date of international publication 平成22年5月20日(2010.5.20)
Priority data
  • 特願2008-292815 (2008.11.14) JP
  • 2009WO-JP68785 (2009.11.2) WO
Title Oligonucleotide derivative, labeling agent and use for labeling agent
Abstract (US8354515)
A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, a C1-4 alkyl or a morpholino group).
Scope of claims [claim1]
1. A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group).
[claim2]
2. The labeling agent according to claim 1, wherein said Y is a condensed polycyclic hydrocarbon group.
[claim3]
3. The labeling agent according to claim 2, wherein said Y is selected from a group consisting of naphthalene, phenathrene, pyrene and perylene.
[claim4]
4. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units in a continuous series.
[claim5]
5. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units, with linker units in between.
[claim6]
6. The labeling agent according to claim 1, wherein said multiple nucleotide units are two nucleotide derivative units in which said Y is the same or different, and are provided in a continuous series.
[claim7]
7. A set of labeling agents comprising two or more labeling agents each provided with a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the labeling agents.
[claim8]
8. The set of labeling agents according to claim 7, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1) above.
[claim9]
9. An oligonucleotide probe comprising a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group).
[claim10]
10. The oligonucleotide probe according to claim 9, comprising said plurality of nucleotide derivative units at the 3' terminal end or 5' terminal end.
[claim11]
11. An oligonucleotide probe set comprising two or more oligonucleotide probes each provided with a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se- or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the probes.
[claim12]
12. The oligonucleotide probe set according to claim 11, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1).
  • Inventor, and Inventor/Applicant
  • UENO YOSHIHITO
  • KITADE YUKIO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO RNA and biofunctions AREA
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