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METHOD FOR COLLECTION OF NUCLEATED RED BLOOD CELLS BY DENSITY-GRADIENT CENTRIFUGATION UTILIZING CHANGE IN DENSITY OF BLOOD CELLS

Foreign code F120006584
File No. S2010-0608
Posted date 2012年5月15日
Country 世界知的所有権機関(WIPO)
International application number 2011JP054414
International publication number WO 2012/023298
Date of international filing 平成23年2月21日(2011.2.21)
Date of international publication 平成24年2月23日(2012.2.23)
Priority data
  • 特願2010-185608 (2010.8.20) JP
Title METHOD FOR COLLECTION OF NUCLEATED RED BLOOD CELLS BY DENSITY-GRADIENT CENTRIFUGATION UTILIZING CHANGE IN DENSITY OF BLOOD CELLS
Abstract Provided is a method for concentrating and collecting fetus-derived nucleated red blood cells contained in a small amount in the blood of a mother body. A method for concentrating and collecting nucleated red blood cells from the blood of a mother body, comprising (i) subjecting the blood of the mother body to first density-gradient centrifugation to collect a cell fraction containing the nucleated red blood cells, (ii) treating the cell fraction containing the nucleated red blood cells in such a manner that the density of the nucleated red blood cells can be varied in a selective manner, thereby avoiding the overlapping of the density of the nucleated red blood cells and the density of white blood cells each other, and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to second density-gradient centrifugation to collect a fraction containing the nucleated red blood cells.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
  • KANAZAWA INSTITUTE OF TECHNOLOGY
  • KANAZAWA MEDICAL UNIVERSITY
  • Inventor
  • TAKAMURA, Yuzuru
  • IDEGAMI, Kotaro
  • KOGI, Mieko
  • TAKABAYASHI, Haruo
IPC(International Patent Classification)
Specified countries AE(UTILITY MODEL),AG,AL(UTILITY MODEL),AM(PROVISIONAL PATENT)(UTILITY MODEL),AO(UTILITY MODEL),AT(UTILITY MODEL),AU,AZ(UTILITY MODEL),BA(CONSENSUAL PATENT),BB,BG(UTILITY MODEL),BH(UTILITY MODEL),BR(UTILITY MODEL),BW,BY(UTILITY MODEL),BZ(UTILITY MODEL),CA,CH,CL(UTILITY MODEL),CN(UTILITY MODEL),CO(UTILITY MODEL),CR(UTILITY MODEL),CU(INVENTOR'S CERTIFICATE),CZ(UTILITY MODEL),DE(UTILITY MODEL),DK(UTILITY MODEL),DM,DO(UTILITY MODEL),DZ,EC(UTILITY MODEL),EE(UTILITY MODEL),EG(UTILITY MODEL),ES(UTILITY MODEL),FI(UTILITY MODEL),GB,GD,GE(UTILITY MODEL),GH(UTILITY CERTIFICATE),GM,GT(UTILITY MODEL),HN(UTILITY MODEL),HR(CONSENSUAL PATENT),HU(UTILITY MODEL),ID,IL,IN,IS,JP(UTILITY MODEL),KE(UTILITY MODEL),KG(UTILITY MODEL),KM,KN,KP(INVENTOR'S CERTIFICATE)(UTILITY MODEL),KR(UTILITY MODEL),KZ(PROVISIONAL PATENT)(UTILITY MODEL),LA,LC,LK,LR,LS(UTILITY MODEL),LT,LU,LY,MA,MD(UTILITY MODEL),ME,MG,MK,MN,MW,MX(UTILITY MODEL),MY(UTILITY-INNOVATION),MZ(UTILITY MODEL),NA,NG,NI(UTILITY MODEL),NO,NZ,OM(UTILITY MODEL),PE(UTILITY MODEL),PG,PH(UTILITY MODEL),PL(UTILITY MODEL),PT(UTILITY MODEL),RO,RS(PETTY PATENT),RU(UTILITY MODEL),SC,SD,SE,SG,SK(UTILITY MODEL),SL(UTILITY MODEL),SM,ST,SV(UTILITY MODEL),SY,TH(PETTY PATENT),TJ(UTILITY MODEL),TM(PROVISIONAL PATENT),TN,TR(UTILITY MODEL),TT(UTILITY CERTIFICATE),TZ,UA(UTILITY MODEL),UG(UTILITY CERTIFICATE),US,UZ(UTILITY MODEL),VC(UTILITY CERTIFICATE),VN(PATENT FOR UTILITY SOLUTION),ZA,ZM,ZW,EP(AL,AT,BE,BG,CH,CY,CZ,DE,DK,EE,ES,FI,FR,GB,GR,HR,HU,IE,IS,IT,LT,LU,LV,MC,MK,MT,NL,NO,PL,PT,RO,RS,SE,SI,SK,SM,TR),OA(BF(UTILITY MODEL),BJ(UTILITY MODEL),CF(UTILITY MODEL),CG(UTILITY MODEL),CI(UTILITY MODEL),CM(UTILITY MODEL),GA(UTILITY MODEL),GN(UTILITY MODEL),GQ(UTILITY MODEL),GW(UTILITY MODEL),ML(UTILITY MODEL),MR(UTILITY MODEL),NE(UTILITY MODEL),SN(UTILITY MODEL),TD(UTILITY MODEL),TG(UTILITY MODEL)),AP(BW(UTILITY MODEL),GH(UTILITY MODEL),GM(UTILITY MODEL),KE(UTILITY MODEL),LR(UTILITY MODEL),LS(UTILITY MODEL),MW(UTILITY MODEL),MZ(UTILITY MODEL),NA(UTILITY MODEL),SD(UTILITY MODEL),SL(UTILITY MODEL),SZ(UTILITY MODEL),TZ(UTILITY MODEL),UG(UTILITY MODEL),ZM(UTILITY MODEL),ZW(UTILITY MODEL)),EA(AM,AZ,BY,KG,KZ,MD,RU,TJ,TM)
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