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METHOD FOR EVALUATING HYDROGEL

Foreign code F200010020
File No. (S2018-0607-N0)
Posted date Jan 30, 2020
Country WIPO
International application number 2019JP020099
International publication number WO 2019225600
Date of international filing May 21, 2019
Date of international publication Nov 28, 2019
Priority data
  • P2018-098312 (May 22, 2018) JP
Title METHOD FOR EVALUATING HYDROGEL
Abstract Provided is a method for evaluating a hydrogel, the method including: a first measurement step for culturing adhesive cells on a hydrogel, staining living cells using a stain which exhibits color in a living cell, and measuring the number of living cells by microscopy; a second measurement step for cleaning the hydrogel after the first measurement step and measuring the number of living cells by microscopy; and an adhesion evaluation step for evaluating adhesion of cells to the hydrogel on the basis of the measurement result of the first measurement step and the measurement result of the second measurement step.
Outline of related art and contending technology BACKGROUND ART
In recent years, three-dimensional network structure having a hydrogel polymer, and medical attention as materials. Hydrogels, the three-dimensional network structure held by a large amount of water.
As the hydrogel, pullulan, dextran, collagen, alginate, polyvinyl alcohol derived from a variety of the hydrogel are known, their mechanical strength, moisture-retaining property, the cell adhesion, such as biocompatible applications in accordance with the characteristics. For example, a cell adhesion of the hydrogel is high, the field of regenerative medicine as a scaffold of the cells can be used. On the other hand, the cell adhesion of the hydrogel, containing a therapeutic agent and the like can be used as a wound dressing.
For example, to Patent Document 1, tissue repair, a cell suitable for use in tissue regeneration can be used as a scaffold of the crosslinked dextran and pullulan mixture of the hydrogel are disclosed.
Scope of claims (In Japanese)[請求項1]
 ハイドロゲル上で接着性細胞を培養し、生細胞内で発色する染色剤を用いて生細胞を染色し、顕微鏡観察により生細胞数を測定する第1測定工程と、
 前記第1測定工程後に前記ハイドロゲルを洗浄し、顕微鏡観察により生細胞数を測定する第2測定工程と、
 前記第1測定工程における測定結果と前記第2測定工程における測定結果とに基づき、前記ハイドロゲルに対する細胞接着性を評価する接着性評価工程と、
を含むハイドロゲルの評価方法。

[請求項2]
 前記第1測定工程及び前記第2測定工程では、プレート状のハイドロゲル基材に設けられた凹部内で前記接着性細胞を培養する、請求項1に記載のハイドロゲルの評価方法。

[請求項3]
 前記第1測定工程における測定結果に基づき、前記ハイドロゲルの細胞毒性を評価する毒性評価工程をさらに含む、請求項1又は2に記載のハイドロゲルの評価方法。

[請求項4]
 前記染色剤がMTTである、請求項1~3のいずれか1項に記載のハイドロゲルの評価方法。

[請求項5]
 ハイドロゲル上で細胞を培養し、生細胞内で発色する染色剤を用いて生細胞を染色し、顕微鏡観察により生細胞数を測定する測定工程と、
 前記測定工程における測定結果に基づき、前記ハイドロゲルの細胞毒性を評価する毒性評価工程と、
を含むハイドロゲルの評価方法。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • TOKYO UNIVERSITY OF SCIENCE FOUNDATION
  • Inventor
  • HANAWA Takehisa
  • KAWANO Yayoi
  • MAMADA Hiroshi
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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