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METHOD FOR GLOMERULAR PODOCYTE INDUCTION, AND METHOD FOR PRODUCING PODOCYTE FROM PLURIPOTENT STEM CELL USING SAID INDUCTION METHOD NEW

外国特許コード F200010025
整理番号 (S2018-0663-N0)
掲載日 2020年1月30日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP021137
国際公開番号 WO 2019230737
国際出願日 令和元年5月28日(2019.5.28)
国際公開日 令和元年12月5日(2019.12.5)
優先権データ
  • 特願2018-102739 (2018.5.29) JP
発明の名称 (英語) METHOD FOR GLOMERULAR PODOCYTE INDUCTION, AND METHOD FOR PRODUCING PODOCYTE FROM PLURIPOTENT STEM CELL USING SAID INDUCTION METHOD NEW
発明の概要(英語) The purpose of the present invention is to provide a method for inducing a glomerular podocytes in vitro. More specifically, the present invention provides a method for producing podocytes, whereby a cell population having a high composition ratio of podocytes can be obtained by selective induction of podocytes. Through the present invention, a method for induced podocyte differentiation is provided, the method including the following steps: step A: a step for culturing a nephron precursor cell or a population thereof in a medium including a Wnt agonist and a ROCK inhibitor; step B: a step for culturing a cell obtained in step A or a population thereof in a medium including Fgf; and step C: a step for culturing a cell obtained in step B or a population thereof in a medium including Fgf (here, the medium in step B and/or the medium in step C includes a TGFβ signal pathway inhibitor).
従来技術、競合技術の概要(英語) BACKGROUND ART
Kidney glomerulus podocyte of the present, an important role in the filtration of blood are responsible for. Podocyte has proteinuria and failure, and then proceeds to the end-stage renal failure eventually needs a dialysis treatment, medical study podocyte important research theme to be a cost.
Podocyte studies, in vitro analysis of immortalized cell lines is used received (non-patent document 1). However, this cell lines gene or protein expression of podocyte characteristic of an extremely low level, carrying out the study problem that insufficient material (non-patent document 2) had. Human iPS cells to be selected from the past to induce podocyte and the reports, a specific gene of podocyte NPHS1 should be prior to induction of the expression level of human iPS cells from 1/10 to 1/5 as compared with the expression remains very low (non-patent document 3-5; Patent Document 1). Therefore, these cells may be induced in a report, referred to as insufficient quality of podocyte considered.
Including reports made by the present inventors, in recent years, human iPS cells from the nephron induced progenitor cells, kidney podocyte and tubule cells created in the test tube containing a plurality of research organoids have been reported (Non-Patent Document 6-9; Patent Document 2). A large number of cell types including renal organoids in the population, an experiment to reduce urinary protein podocyte fault model for drug screening for therapeutic drug development for, and a more refined podocyte of the cell (cell-autonomous) autonomous verification of the effect are required. Therefore, a high-purity podocyte efficiently from the cell population to induce the differentiation of a method of manufacturing or is demanded.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY
  • 発明者(英語)
  • NISHINAKAMURA Ryuichi
  • TAGUCHI Atsuhiro
  • YOSHIMURA Yasuhiro
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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