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METHOD FOR SYNTHESIZING ENZYME FOR β-GLYCOSIDE OF LACTO-N-BIOSE I OR GALACTO-N-BIOSE USING MODIFIED PHOSPHORYLASE 新技術説明会

外国特許コード F200010049
整理番号 6192
掲載日 2020年5月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP032866
国際公開番号 WO 2020040257
国際出願日 令和元年8月22日(2019.8.22)
国際公開日 令和2年2月27日(2020.2.27)
優先権データ
  • 特願2018-157840 (2018.8.24) JP
発明の名称 (英語) METHOD FOR SYNTHESIZING ENZYME FOR β-GLYCOSIDE OF LACTO-N-BIOSE I OR GALACTO-N-BIOSE USING MODIFIED PHOSPHORYLASE 新技術説明会
発明の概要(英語) This application provides: a modified GLNBP protein in which a mutation is introduced to a certain region of a non-mutant GLNBP; and a method of forming a β-1,3 bond between the 1-position of a galactose-1-phosphate using the modified GLNBP, and the 3-position of an N-acetylglucosamine residue or an N-acetylgalactosamine residue in a saccharide having the N-acetylglucosamine residue or the N-acetylgalactosamine residue which is β-bound to a non-reducing end thereof, and also having one or multiple saccharide residues.
従来技術、競合技術の概要(英語) BACKGROUND ART
Milk nutrient infant intestinal flora is dominant bifidobacterium is formed, the human milk oligosaccharides which is included in the involved components. Among the oligosaccharide moieties, tetraose -N- lacto (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) was incubated with the true known factor. The formation of an infant intestinal flora, adult obesity after a large impact on the allergies known, WHO may recommend to the gnotobiotic breast milk, breast milk gnotobiotic been made are reduced, also to the premature timing of the weaning. Also, cesarean rate exceeds 25% all over the world, the infant intestinal flora the delivery is a vaginal birth of the infant can be significantly different from that known in the art. For this reason, close to human milk to milk prepared and the urgent need for the development. However, the existing -N- lacto tetraose as a synthesis method using chemical synthesis and metabolic engineering (for example non-patent document 1) fermentation methods have been developed however, has not been practical either.
Β-1, 3-N - galactosyl (EC 2.4.1.211 acetylhexosamine phosphorylase; a.k.a.: -N- galacto -N- lacto-biose biose/phosphorylase I) (hereinafter, sometimes abbreviated as GLNBP) is, lacto-biose I - N-1-phosphate (Galβ1-3GlcNAc) galactose (Gal-1-P) (GlcNAc) and the N- acetylglucosamine, and galactose -N- galacto-biose-1-phosphate (Galβ1-3GalNAc) (Gal-1-P) (GalNAc) and the N- acetylgalactosamine, and its reverse reaction of the decomposition pressure of phosphoric acid having a catalytic activity is known as an enzyme, its presence is Bifidobacterium many bacteria Rhoma widely been found. However, such as - N-N - lacto-biose tetraose GLNBP is β - glycoside of lacto-I do not act, using its reverse reaction of these compounds may not be prepared.
GLNBP the structure of much research has been made. For example the following may be referred to as lines and Bifidobacterium longum subsp. longum (strain ATCC 15707/DSM 20219/JCM 1217/NCTC 11818/E194b) (, Bifidobacterium longum JCM1217) in the result and from the storage stability, Bifidobacterium sp 27 in the helix of the structure has been suggested (Non-Patent Document 2). The three-dimensional structure 3 in the center of activity than have been reported the results of a study (for example Non-Patent Document 3).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KYOTO UNIVERSITY
  • NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
  • 発明者(英語)
  • KATAYAMA, Takane
  • KATOH, Toshihiko
  • KITAOKA Motomitsu
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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