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Bioparticle observation apparatus and bioparticle observation method

Foreign code F200010111
File No. 6211
Posted date May 18, 2020
Country China
Application number 201910502109
Gazette No. 110577890
Date of filing Jun 11, 2019
Gazette Date Dec 17, 2019
Priority data
  • P2018-111342 (Jun 11, 2018) JP
Title Bioparticle observation apparatus and bioparticle observation method
Abstract The invention provides a bioparticle observation apparatus and the like that are able to reduce damage to bioparticles and facilitate observation of a single bioparticle. A bioparticle observation apparatus (101) includes a dielectrophoresis electrode (104) that outputs a first signal causing a dielectrophoresis force to act on a bioparticle (106), a sensor electrode (105) that detects an impedance difference between the bioparticle (106) and the liquid (102), and a control circuit (110) that controls the first signal so that the detected impedance difference is fixed.
Outline of related art and contending technology BACKGROUND ART
Background
cells, which are one of biological particles, are roughly classified into adhesive cells that are attached to a culture container and proliferate, and planktonic cells that proliferate in a state of floating in a culture medium, depending on the mode of existence in cell culture.
In order to follow the growth pattern of floating cells such as hematopoietic cells, it is necessary to fix an observation target at a predetermined position when observing the cells using a microscope. In order to observe the floating cells under a microscope, a method of distributing the floating cells from the inside of a culture medium in a dish or the like and observing the cells is considered, but the cells are largely damaged because the floating state cannot be maintained for a long period of time in culture or observation under a microscope.
on the other hand, as shown in fig. 7, a method of immobilizing planktonic cells on a cell surface, a plate 202, or the like by forming an immobilizing agent 201 such as a protein or a polymer having a good affinity with the surface of the cells 200 in a solution 203 is known. Since the cell wall of the cell 200 is not directly adhered to the bottom surface of the tray 202 or the like, the floating state can be maintained even when the culture or observation is performed for a long time. Viewing is performed across a cover 204 of a cover slip or the like. It is predicted that the above-described constitution causes little damage to cells. For example, patent document 1 proposes a method in which a fixative 201 has a phosphorylcholine-like group and a hydrazide group. In this method, the planktonic cell-immobilizing protein is modified in a dish or the like in advance, and is bound to the surface of planktonic cells and mixed and immobilized. This allows the surface of the cell 200, the disk 202, and the like to be fixed.
documents of the prior art
Patent document
Patent document 1: japanese patent laid-open publication No. 2005-80579 (published 3/31/2005) "
Scope of claims [claim1]
1. A biological particle observation apparatus for observing biological particles in a liquid, comprising:
A dielectrophoresis electrode that outputs a first signal for causing a dielectrophoretic force to act on the biological particles;
A sensor electrode for detecting an impedance difference between the biological particle and the liquid; and
And a control circuit for controlling the first signal so that the detected impedance difference is constant.

[claim2]
2. The biological particle observation apparatus according to claim 1,
The control circuit controls the amplitude or frequency of the first signal.

[claim3]
3. The biological particle observation apparatus according to claim 1 or 2,
The dielectrophoresis electrode has a shape surrounding the sensor electrode with a circle or a polygon centered on the sensor electrode, and outputs a signal that is a negative dielectrophoresis force to the biological particles as the first signal.

[claim4]
4. The biological particle observation apparatus according to claim 1 or 2,
The dielectrophoresis electrode is configured by a plurality of electrodes arranged so as to surround the sensor electrode with the sensor electrode as a center, and outputs a signal that is a negative dielectrophoresis force as the first signal to the biological particles.

[claim5]
5. the biological particle observation apparatus according to claim 1 or 2,
The sensor electrode is a single electrode or a differential electrode.

[claim6]
6. The biological particle observation apparatus according to claim 1 or 2,
the sensor electrode is connected to a switch, and the function of detecting the impedance difference and the function of outputting a signal that is a positive dielectrophoretic force to the biological particles can be switched via the switch.

[claim7]
7. The biological particle observation apparatus according to claim 1 or 2,
Outputting a second signal from the sensor electrode that becomes a positive dielectrophoretic force.

[claim8]
8. The biological particle observation apparatus according to claim 1 or 2,
The apparatus is provided with a microscope which can observe the state that the biological particles stay at a predetermined position from the outside.

[claim9]
9. The biological particle observation apparatus according to claim 1 or 2,
A microfluidic channel into which the biological particles flow is provided.

[claim10]
10. A method of observing biological particles, characterized in that,
The biological particle observation apparatus according to claim 1 or 2, wherein the biological particle is held at a predetermined position in the liquid;
The state where the biological particles stay at the predetermined position is observed from the outside via a microscope.
  • Applicant
  • KYOTO UNIVERSITY
  • SHARP CORPORATION
  • Inventor
  • MITSUNAKA TAKESHI
  • OGAWA YUICHI
IPC(International Patent Classification)
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