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GENE-SPECIFIC NON-BIASED AMPLIFICATION METHOD

Foreign code F200010134
Posted date May 22, 2020
Country EPO
Application number 16755460
Gazette No. 3263716
Date of filing Feb 23, 2016
Gazette Date Jan 3, 2018
International application number JP2016055192
International publication number WO2016136716
Date of international filing Feb 23, 2016
Date of international publication Sep 1, 2016
Priority data
  • P2015-033236 (Feb 23, 2015) JP
Title GENE-SPECIFIC NON-BIASED AMPLIFICATION METHOD
Abstract The present invention is intended to provide a method of amplifying a target gene without bias and an adapter DNA use therefor. The adapter DNA of the present invention is double-stranded adapter DNA, which is used for unbiased gene amplification and has the following features: (a) the double-stranded adapter DNA has a sense strand and an antisense strand that are annealed with each other, the base length of the sense strand being equal to or longer than the base length of the antisense strand; (b) the base length of the sense strand is 15 to 40 bp; (c) the antisense strand includes a plurality of uracil bases, each uracil is removed by treating an adapter portion with uracil DNA glycosylase (UNG), and then, heat treatment is performed to degrade the antisense strand; (d) at least one end of the adapter DNA is in the form of a blunt end; (e) the other end of the adapter DNA binds to a target gene to be amplified; and (f) a part or all of the sense strand corresponds to a forward primer sequence used for gene amplification.
  • Applicant
  • Tohoku University
  • Inventor
  • OGASAWARA, Koetsu
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