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MICROSCOPE DEVICE AND FLUORESCENT OBSERVING METHOD USING SAME UPDATE

外国特許コード F200010137
整理番号 B115-02WO
掲載日 2020年6月1日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2009JP060644
国際公開番号 WO 2010029799
国際出願日 平成21年6月10日(2009.6.10)
国際公開日 平成22年3月18日(2010.3.18)
優先権データ
  • 特願2008-235795 (2008.9.13) JP
発明の名称 (英語) MICROSCOPE DEVICE AND FLUORESCENT OBSERVING METHOD USING SAME UPDATE
発明の概要(英語) A microscope device and a fluorescent observing method both for controlling the position of an object to be observed such as cells and controlling fluorescence records. The microscope device (1) includes a stage (3) on which the object (2) is placed, a light source (4) for illuminating the object (2), an exciting light source (5) for causing the object (2) to emit fluorescence F, an image information detecting section (16) for detecting image information on the basis of light T produced by the object (2), a fluorescent image information detecting section (17) for detecting fluorescent image information attributed to the fluorescence F, and a control section (20) for determining a fluorescent observation region of the object (2) according to a motion model of the object (2) and the image information about the object (2) inputted from the image information detecting section (16) and acquiring the image information about the object (2) and the fluorescent image information from portions spaced at predetermined distances in the fluorescent observation region.
従来技術、競合技術の概要(英語) BACKGROUND ART
Such as in the field of molecular biology, intracellular ion such a living body tissue, the observation of the molecules is an essential technique. An optical microscope is, when observing the biological tissue mainly used. In addition, the laser confocal microscope, light is irradiated to a part of a living body, moving the irradiation position, which is used when a part of a cell is observed.
One of the main optical microscope observation technique, ions and molecules within the cells of the biological tissue with a fluorescent dye, excitation light is irradiated onto the fluorescent dye, the fluorescent dye is excited by observing the fluorescence emitted by the fluorescence observation method. Is different from the wavelength of the excitation light and the fluorescence. Therefore, by analyzing the fluorescence detection or measurement of the concentration of intracellular molecules can be achieved. Therefore, the fluorescence observation has the following advantages. (i) The magnitude of the ion or molecule can also be observed. (ii) Can be observed only a molecule or a specific ion. (Iii) ion or a molecular brightness of fluorescence changes in response to the concentration, the concentration of these can be measured.
However, in general fluorescence observation has the following disadvantage. (i) In the case of the ultraviolet wavelength band of the excitation light, excitation light may be toxic to cells. (ii) Excitation light continues to fade the fluorescent dye is placed. Discoloredregion is, the intensity of the fluorescence is weak.
Fluorescence observation can be moved using a fluorescent microscope and observing the cells, the cells out of the microscope field of view if the observation is interrupted.
A solution to this problem as the magnification of the objective lens, (1) to increase the field to reduce the mechanical or chemical, (2) to suppress the movement of the cell, (3) moving the stage to the location of the center of the field. However, (1) the spatial resolution is reduced and the method of the method, (2) can adversely affect cells.
Patent Document 1 and 2 with respect to the solution described (3) is a method, in order to observe a minute observation object includes a mechanism for tracking the observed object has been disclosed an optical microscope. The optical microscope, with the use of the transmitted light and the observation position of the transmission light in the control area and the recording can be performed.
In the Patent Document 3-5, the fluorescence of the cell and fluorescent recording and control of the position and the observation area can be performed using a fluorescence microscope is disclosed.
Is the non-patent document 1-5, a relatively large observation object observed in the field of view moves as paramecium fixed to the reported method.
To move freely in a culture medium of cells capable of, medical and biological importance such as those in the art, having a function to protect the human body from the bacteria and virus and immune cells (see Non-Patent Document 6).
Associated with immune function are present in many intracellular molecules, the molecules can be detected and the time variation of the molecule concentration is measured by the fluorescence observation must be used.
Among the immune cells, and does not adhere too much to the surface of the glass slide, the suspended cells are classified into floating cells. Floating cells are placed in a Petri dish, a culture solution convection, gravity, walls and the bottom of the petri dish, such as by the interaction between the cell and the water surface, always has changed. Observation of the floating cells, for example Non-Patent Document 1 have been reported in the technique using the back, a method of tracking a single cell is also conceivable.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • IGARASHI Yasunobu
  • OBARA Takeshi
  • DEGUCHI Yuki
  • SUZUKI Takeshi
  • HASHIMOTO Koichi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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