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UNDIFFERENTIATED CELL DETECTION METHOD

外国特許コード F200010147
整理番号 (S2018-0734-N0)
掲載日 2020年6月2日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP023599
国際公開番号 WO 2019240247
国際出願日 令和元年6月14日(2019.6.14)
国際公開日 令和元年12月19日(2019.12.19)
優先権データ
  • 特願2018-115025 (2018.6.15) JP
発明の名称 (英語) UNDIFFERENTIATED CELL DETECTION METHOD
発明の概要(英語) Provided is a marker gene capable of detecting the persistence/admixture of undifferentiated cells in a differentiated cell population. Undifferentiated cells present in a differentiated cell population are detected by using at least one gene selected from the group consisting of ESRG, VSNL1, THY1, SFRP2, SPP1, USP44, and CNMD as an undifferentiation marker. A method for detecting undifferentiated cells, a method of use as an undifferentiation marker, and an undifferentiated cell detection kit. Also provided is a method for selecting an undifferentiated cell line.
従来技術、競合技術の概要(英語) BACKGROUND ART
Used in regenerative medicine iPS undifferentiated population of differentiated cells into/leaving the cell is detected, evaluating the risk of cancer is very important from the viewpoint. Up to this point, leaving the cell to detect tampering/iPS undifferentiated, quantitative PCR (qPCR) is a technique for evaluating iPS cell-specific genes by a method of detecting a (non-patent document 1), differentiated cells in culture undifferentiated cells due to the maintaining condition reculturing method (Non-Patent Document 2) has been reported.
In the prior art, the method reculturing, iPS cell colony from undifferentiated mixed to form a high accuracy because there is an advantage that the other hand, from 1 weeks to detect, using the quantitative PCR method is convenient, in view of excellent performed rapidly.
However, this is reported (Non-Patent Document 1) of the LIN28 organ/tissue of the differentiated cells with a low expression (retinal pigment epithelial cells) exists on the other hand, differentiated cells or iPS cells such as hepatocytes differentiated from the induced cells can be seen from the expression, various differentiated cells and undifferentiated iPS cells subject of the detection of the remaining mixed/may not be available if there is a problem.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • TANIGUCHI HIDEKI
  • SEKINE KEISUKE
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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