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MUCONIC ACID-PRODUCING TRANSFORMED MICROORGANISM AND USE THEREOF NEW

外国特許コード F200010202
整理番号 (S2019-0033-N0)
掲載日 2020年7月30日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2019JP040908
国際公開番号 WO 2020080467
国際出願日 令和元年10月17日(2019.10.17)
国際公開日 令和2年4月23日(2020.4.23)
優先権データ
  • 特願2018-196001 (2018.10.17) JP
発明の名称 (英語) MUCONIC ACID-PRODUCING TRANSFORMED MICROORGANISM AND USE THEREOF NEW
発明の概要(英語) The purpose of the present invention is to provide: a microorganism that allows production of muconic acid from a lignin-derived aromatic compound at low cost and irrespective of the type of lignin; and a method for producing muconic acid using the microorganism. The purpose is achieved by: a transformed microorganism obtained from a host microorganism that belongs to the genus Pseudomonas , that has the pcaH gene, pcaG gene, catA gene, and catB gene on the chromosome thereof, and that uses a syringyl lignin-derived aromatic compound, wherein the catB gene and at least one gene selected from the group consisting of the pcaH gene and the pcaG gene are deleted from the chromosome, and an inserted aroY gene is expressed; and a method for producing muconic acid using the transformed microorganism.
従来技術、競合技術の概要(英語) BACKGROUND ART
Vascular plant cell wall components of lignin as the amorphous polymer substance is present, the complexity of the configuration of the fuzed unit-phenylpropane and, on the chemical structure containing a methoxy group are the features. Wood lignins are stuck to each other and to a plant cell, and serves to enhance the tissue, in the wood chips about 18%-36%, about 15%-25% in the presence of the herbaceous. Therefore, to effectively utilize wood, and decomposition of lignin, an attempt to obtain useful compounds have been made various. Such as wood lignin derived from biomass, p- hydroxyphenyl lignin, syringyl lignin guay acyl lignin and 3 of the one to be known.
On the other hand, cis, cis - muconic acid (hereinafter, simply sometimes referred to as muconic acid.) Is, the carboxyl group and double bond in the molecule by 2 pieces, is a compound having high reactivity. Muconic acid as a starting material of the various known and muconic acid derivatives, for example, lactone, sulfone, polyamide, polyester, a thioester, additional polymers and the like. Such as muconic acid derivatives, various applications are known as having, for example, surfactants, flame retardants, UV light stabilizers, thermosetting plastics, may be used as coating agents.
In this way, muconic acid are, muconic acid derivatives are subjected to a variety of applications in the form of a result, lignin muconic acid can be produced, the reproduction of the resources is achieved, very useful. Therefore, a material derived from lignin or a lignin, muconic acid has been attempted a method of manufacturing. Particularly as such a method, the microorganisms of the bioconversion is used has been studied.
For example, non-patent document 1 (as disclosed herein all of the literature is incorporated by reference.) Is, Pseudomonas, a host microorganism (Pseudomonas putida) putida, a gene on a chromosome and gene pcaH pcaG (hereinafter, sometimes referred to as gene pcaHG with.) As well as gene catR, catB gene, the gene and catC catA gene disruption, and, inserted into the gene aroY catA gene, a gene or gene ecdB catA aroY gene expression to produce a transformed microorganisms, the microorganisms grown transformed glucose, caffeic acid then was muconic acid p- is described.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • HIROSAKI UNIVERSITY
  • NAGAOKA UNIVERSITY OF TECHNOLOGY
  • 発明者(英語)
  • SONOKI, Tomonori
  • MASAI, Eiji
  • KAMIMURA, Naofumi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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