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METHOD FOR PRODUCING OSTEOBLAST CLUSTER USING IPS CELLS NEW

外国特許コード F200010231
整理番号 (S2019-0268-N0)
掲載日 2020年10月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2020JP007876
国際公開番号 WO2020175592
国際出願日 令和2年2月26日(2020.2.26)
国際公開日 令和2年9月3日(2020.9.3)
優先権データ
  • 特願2019-033277 (2019.2.26) JP
発明の名称 (英語) METHOD FOR PRODUCING OSTEOBLAST CLUSTER USING IPS CELLS NEW
発明の概要(英語) A method for producing an osteoblast cluster from iPS cells, said method comprising: (1) a step for inducing the formation of embryoid bodies by non-adhesively culturing undifferentiated iPS cells; (2) a step for inducing the differentiation of the iPS cells into mesodermal cells by non-adhesively culturing the embryoid bodies of the iPS cells obtained in step (1); and (3) a step for inducing the differentiation into osteoblasts by non-adhesively culturing the mesodermal cells of the iPS cells obtained in step (2), wherein the aforesaid steps (1) and (2) are carried out using a culture container which is provided with a bottom face and a circular side wall standing from the bottom face and in which a plurality of concave portions are formed in the bottom face in such a manner as to be independent from each other.
従来技術、競合技術の概要(英語) Background Art
The demand for artificial bone/bone prosthetic materials for supplementing bone tumor extraction, crushed bone fracture, bone defects associated with fixation of rheumatoid arthritis, bone defects lost due to maxilla resorption, etc., has been very increasing.
At present, non-resorbable materials such as hydroxyapatite and resorbable materials such as / S-3-th calcium phosphate, which are currently used as artificial bone/bone prosthetic materials in clinical use, have problems such as lack of osteoinductive properties compared to autologous bone, and thus have not always good prognosis of surgical procedures. A hybrid artificial bone obtained by combining a human e bone desired to be developed and promoted as a next generation type with a growth factor protein such as BMP (B o n e Mo r p h o g e n e t i c P r o t e i n) is also provided. Since the bone prosthetic material has no "extracellular matrix" important for bone tissue regeneration, sufficient bone regeneration effect cannot be obtained.
Under such circumstances, the inventors succeed in producing a bone regeneration agent using stem cells as a raw material, comprising inactivated cell clusters, wherein the inactivated cell clusters comprise at least calcified products and extracellular matrix.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOHOKU UNIVERSITY
  • 発明者(英語)
  • EGUSA, Hiroshi
  • OKAWA, Hiroko
  • HORIE, Naohiro
  • KONDO, Takeru
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN WS ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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