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PREPARATION AND EXPANSION CULTURE OF ENDOTHELIAL PROGENITOR CELL NEW

外国特許コード F200010250
整理番号 (S2019-0238-N0)
掲載日 2020年10月30日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2020JP005255
国際公開番号 WO2020179380
国際出願日 令和2年2月12日(2020.2.12)
国際公開日 令和2年9月10日(2020.9.10)
優先権データ
  • 特願2019-040117 (2019.3.6) JP
発明の名称 (英語) PREPARATION AND EXPANSION CULTURE OF ENDOTHELIAL PROGENITOR CELL NEW
発明の概要(英語) The present invention addresses the problem of providing a means for easily preparing endothelial progenitor cells at high purity and low cost. The present invention also addresses the problem of providing a method for efficiently proliferating endothelial progenitor cells. Endothelial progenitor cells are prepared at high purity by the steps of: differentiating pluripotent stem cells into endothelial progenitor cells; and purifying the endothelial progenitor cells using the difference in adhesion ability between the endothelial progenitor cells of the cell population obtained in the previous step and other cells. Meanwhile, the endothelial progenitor cells are cultured and proliferated in the presence of a ROCK inhibitor, a GSK-3β inhibitor, and a TGF-β receptor inhibitor as well as a basic fibroblast growth factor and an epidermal growth factor.
従来技術、競合技術の概要(英語) BACKGROUND ART
Human pluripotent stem cells including induced pluripotent stem cells (induced pluripotent stem cells , iPS cells) and embryonic stem cells (embryonic stem cells , ES cells) proliferate infinitely and have the ability to differentiate into any site of the human body. Because of this characteristic, human pluripotent stem cells (, particularly iPS cells), are expected to be used for the construction of drug screening models simulating human organs and clinical applications in regenerative medicine.
Various clinical applications of the iPS cell-derived vascular endothelial progenitor cells (EPCs) may be considered, such as blood vessel regeneration, myocardial infarction treatment by co-transplantation with myocardium, and three-dimensional cellular tissue construction of advanced organs. In addition, it is expected to construct a pathological model of vascular diseases and to use it as a screening system in developing new drugs by differentiating it into brain capillary endothelial cells. A large amount of high-purity vascular endothelial progenitor cells are required to be used for such purposes.
Reference) is now made to (, for example, Non-Patent Documents 1 and 2, where many reports have been made that vascular endothelial cells (ECs) and vascular endothelial progenitor cells are efficiently differentiated and induced from ES and iPS cells. However, since the rate of differentiation into vascular endothelial cells and vascular endothelial progenitor cells is greatly affected by the cell line used and the state before differentiation, it is often purified by using antibody beads/magnetic beads or cell sorter in order to obtain high-purity cells. In addition, efficient expansion culture of iPS cell-derived vascular endothelial progenitor cells has been reported. The research group of the present inventors reported a novel method for inducing differentiation of vascular endothelial progenitor cells by applying iPS-sac method in the previous patent application (Patent Document 1). Patent Document 2 discloses a method for preparing vascular endothelial progenitor cells from embryonic stem cells.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NAGOYA CITY UNIVERSITY
  • 発明者(英語)
  • MATSUNAGA Tamihide
  • HASHITA Tadahiro
  • AOKI Hiromasa
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN WS ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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