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PRODUCTION METHOD AND KIT OF INDUCED PLURIPOTENT STEM CELLS NEW

外国特許コード F210010283
整理番号 (S2019-0335-N0)
掲載日 2021年1月28日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2020JP016924
国際公開番号 WO 2020213725
国際出願日 令和2年4月17日(2020.4.17)
国際公開日 令和2年10月22日(2020.10.22)
優先権データ
  • 特願2019-078907 (2019.4.17) JP
発明の名称 (英語) PRODUCTION METHOD AND KIT OF INDUCED PLURIPOTENT STEM CELLS NEW
発明の概要(英語) This method of producing induced pluripotent stem cells involves a step for introducing an initialization factor into somatic cells of a mammal, and culturing in a neural stem cell culture medium to obtain induced neural stem cell-like cells, and a step for cultivating said induced neural stem cell-like cells in a growth medium to obtain induced pluripotent stem cells , wherein the initialization factor contains an OCT family, a SOX family, a KLF family, a MYC family, a LIN28 family and a P53 function inhibitor.
従来技術、競合技術の概要(英語) BACKGROUND ART
Techniques for establishing iPS cells from human and mouse somatic cells have been improved and simplified, and the efficiency of establishing iPS cells has been improved. On the other hand, there is an animal (pluripotent induction resistant animal) which is difficult to establish iPS cells from adult somatic cells. Dogs, pigs, marmosets, cats, cows, horses, and the like are known as pluripotent induction resistant animals.
See), for example, (, Non-Patent Document 1, in which canine iPS cells can be established by persistently expressing a reprogramming factor in canine fetus fibroblasts. In addition, see), for example, Non-Patent Document 2, it is possible to establish canine iPS cells from canine fetus fibroblasts by using Sendai virus. See), for example, Non-Patent Document 3, in which porcine iPS cells can be established by persistently expressing a reprogramming factor in porcine fetus fibroblasts.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KEIO UNIVERSITY
  • NIHON UNIVERSITY
  • 発明者(英語)
  • OKANO Hideyuki
  • SHIOZAWA Seiji
  • YOSHIMATSU Sho
  • EDAMURA Kazuya
  • IGUCHI Aozora
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN WS ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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