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Novel microalgae and use for same NEW

外国特許コード F210010329
整理番号 AF30-04WO
掲載日 2021年2月2日
出願国 欧州特許庁(EPO)
出願番号 18884061
公報番号 3719114
出願日 平成30年11月28日(2018.11.28)
公報発行日 令和2年10月7日(2020.10.7)
国際出願番号 JP2018043696
国際公開番号 WO2019107385
国際出願日 平成30年11月28日(2018.11.28)
国際公開日 令和元年6月6日(2019.6.6)
優先権データ
  • 特願2017-228394 (2017.11.28) JP
  • 特願2017-228396 (2017.11.28) JP
  • 特願2018-101753 (2018.5.28) JP
  • 特願2018-177416 (2018.9.21) JP
  • 2018JP43696 (2018.11.28) WO
発明の名称 (英語) Novel microalgae and use for same NEW
発明の概要(英語) Provided is an alga belonging to Cyanidiophyceae having a diploid cell and a haploid cell form. Also provided is a nutrient composition containing an alga belonging to Cyanidiophyceae or an extract thereof.
従来技術、競合技術の概要(英語) Background Art]
Microalgae have a carbon dioxide fixation capacity higher than that of terrestrial plants and do not compete with agricultural products for their growing places. Therefore, some species of the microalgae are cultured in large quantities and used industrially as feed, functional foods, cosmetic materials, and the like.
In a case where the microalgae are used industrially, in view of costs and the like, microalgae that can be cultured outdoors in large quantities are desirable. However, the microalgae that can be cultured outdoors in large quantities need to meet requirements such as being resistant to environmental changes (light, temperature, and the like), being cultivable under conditions where other organisms cannot survive, and being able to grow to high density. Accordingly, hitherto, only a few species such as Chlorella, Euglena, Dunaliella, and Spirulina have been practically used in industry.
One of the characteristic of the aforementioned species of algae is that they can be cultured in an environment, such as an environment with a high salt concentration, high pH, and low pH, where other organisms are difficult to grow. These species of algae are rich in amino acids and vitamins and are used as raw materials for functional foods or supplements.
Meanwhile, the algae belonging to Cyanidiophyceae, which are unicellular primitive red algae, grow preferentially in acidic hot springs containing sulfuric acid. Cyanidiophyceae includes Cyanidioschyzon, Cyanidium, and Galdieria (Non-Patent Document 1). As haploid algae, only Cyanidioschyzon merolae is known (Non-Patent Document 2). Cyanidioschyzon merolae has no rigid cell wall (Non-Patent Documents 1 and 2).
Cyanidioschyzon merolae is constituted with a very simple set of cell organelles, and the genome sequence thereof has been completely decoded. Therefore, Cyanidioschyzon merolae is used as a model organism for basic research on photosynthetic organisms, and the development of techniques for modifying the gene thereof is also making progress (Non-Patent Documents 3 and 4).
[Citation List]
[Non-Patent Literature]
[Non-Patent Document 1]
Pinto, G. (2007) Cyanidiophyceae: looking back-looking forward, In: J. Seckbach (ed.) Algae and Cyanobacteria in Extreme Environments.Springer, Dordrecht, The Netherlands, pp. 389-397.
[Non-Patent Document 2]
Misumi O et al. (2005) Cyanidioschyzon merolae Genome. A Tool for Facilitating Comparable Studies on Organelle Biogenesis in Photosynthetic Eukaryotes. Plant Physiol. 137 (2): 567-585.
[Non-Patent Document 3]
Fujiwara T et al. (2013) Gene targeting in the red alga Cyanidioschyzon merolae: single- and multi-copy insertion using authentic and chimeric selection markers. PLOS ONE. Sep 5; 8 (9): e73608.
[Non-Patent Document 4]
Fujiwara T et al. (2015) A nitrogen source-dependent inducible and repressible gene expression system in the red alga Cyanidioschyzon merolae. Front Plant Sci. Aug 26; 6: 657.
特許請求の範囲(英語) [claim1]
1. An alga belonging to Cyanidiophyceae having a diploid cell form and a haploid cell form.

[claim2]
2. The alga according to claim 1,
wherein the haploid cell form is ruptured under a condition of pH 7.

[claim3]
3. The alga according to claim 1 or 2,
wherein a ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene has identity equal to or higher than 90% with a base sequence shown in SEQ ID NO: 3 or 4.

[claim4]
4. The alga according to claim 3, which is selected from the group consisting of a Cyanidium sp. YFU3 strain (FERM BP-22334), a Cyanidium sp. HKN1 strain (FERM BP-22333), and a mutant of these.

[claim5]
5. The alga according to any one of claims 1 to 4, which is a transformant.

[claim6]
6. The alga according to claim 5,
wherein the transformant is prepared by self-cloning.

[claim7]
7. The alga according to any one of claims 1 to 6, which is the haploid cell form.

[claim8]
8. A method for producing a haploid alga, comprising:
(a) step of culturing a diploid cell of the alga according to any one of claims 1 to 6; and
(b) step of isolating a haploid cell generated during the culturing.

[claim9]
9. The method for producing a haploid alga according to claim 8,
wherein the culturing in the step (a) is performed under a condition of temperature of 30°C to 50°C, pH of 1.0 to 5.0, and CO2 concentration of 1% to 3%.

[claim10]
10. An alga culture comprising: the alga according to any one of claims 1 to 7,
wherein a ratio of the number of haploid cells of the alga to the total number of cells of the alga contained in the alga culture is 70% to 100%.

[claim11]
11. A low-temperature treatment product obtained by performing a drying and swelling treatment on the alga according to any one of claims 1 to 7.

[claim12]
12. A nutrient composition comprising:
an alga belonging to Cyanidiophyceae or an extract thereof.

[claim13]
13. The nutrient composition according to claim 12,
wherein the alga is a haploid alga.

[claim14]
14. The nutrient composition according to claim 12 or 13,
wherein the alga undergoes cell rupture under a condition of pH 7.

[claim15]
15. The nutrient composition according to any one of claims 12 to 14,
wherein the alga belongs to Cyanidioschyzon.

[claim16]
16. The nutrient composition according to any one of claims 12 to 14,
wherein the alga is the alga according to any one of claims 1 to 7.

[claim17]
17. The nutrient composition according to any one of claims 12 to 16,
wherein the alga is a transformant having an increased intracellular content of at least one kind of nutrient.

[claim18]
18. The nutrient composition according to claim 17,
wherein the transformant is prepared by self-cloning.

[claim19]
19. The nutrient composition according to any one of claims 12 to 18, comprising:
at least one kind of nutrient selected from the group consisting of amino acids, vitamins, proteins, lipids, and dietary fiber.

[claim20]
20. A food comprising:
the nutrient composition according to any one of claims 12 to 19.

[claim21]
21. The food according to claim 20, which is a functional food or a dietary supplement.

[claim22]
22. A feed or a pet food comprising:
the nutrient composition according to any one of claims 12 to 19.

[claim23]
23. A cosmetic comprising:
the nutrient composition according to any one of claims 12 to 19.

[claim24]
24. A method for producing a nutrient, comprising:
(a) step of a disrupting cell of an alga belonging to Cyanidiophyceae so as to obtain a cell disruption product; and
(b) step of separating at least one kind of nutrient from the cell disruption product.

[claim25]
25. The method for producing a nutrient according to claim 24,
wherein the nutrient is at least one kind of nutrient selected from the group consisting of amino acids, vitamins, proteins, lipids, and dietary fiber.

[claim26]
26. The method for producing a nutrient according to claim 25,
wherein the amino acids are at least one kind of amino acid selected from the group consisting of isoleucine, leucine, lysine, methionine, cystine, phenylalanine, tyrosine, threonine, tryptophan, valine, arginine, histidine, alanine, aspartic acid, glutamic acid, glycine, proline, serine, and γ-aminobutyric acid.

[claim27]
27. The method for producing a nutrient according to claim 25,
wherein the vitamins are at least one kind of vitamin selected from the group consisting of vitamin A, β-carotene, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin E, vitamin K1, vitamin K2, niacin, inositol, folic acid, and biotin.

[claim28]
28. The method for producing a nutrient according to any one of claims 24 to 27,
wherein the alga is a haploid alga.

[claim29]
29. The method for producing a nutrient according to any one of claims 24 to 28,
wherein the alga undergoes cell rupture under a condition of pH 7.

[claim30]
30. The method for producing a nutrient according to any one of claims 24 to 29,
wherein the alga belong to Cyanidioschyzon.

[claim31]
31. The method for producing a nutrient according to any one of claims 24 to 29,
wherein the alga is the alga according to any one of claims 1 to 7.

[claim32]
32. The method for producing a nutrient according to any one of claims 24 to 30,
wherein the alga is a transformant having an increased intracellular content of at least one kind of nutrient.

[claim33]
33. The method for producing a nutrient according to claim 32,
wherein the transformant is prepared by self-cloning.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MIYAGISHIMA SHIN-YA
  • HIROOKA SHUNSUKE
国際特許分類(IPC)
指定国 Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Extension States: BA ME
参考情報 (研究プロジェクト等) CREST Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms AREA
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