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NOVEL MICROALGAE AND USE FOR SAME NEW

外国特許コード F210010330
整理番号 AF30-04WO
掲載日 2021年2月2日
出願国 大韓民国
出願番号 20207018058
公報番号 20200115476
出願日 平成30年11月28日(2018.11.28)
公報発行日 令和2年10月7日(2020.10.7)
国際出願番号 JP2018043696
国際公開番号 WO2019107385
国際出願日 平成30年11月28日(2018.11.28)
国際公開日 令和元年6月6日(2019.6.6)
優先権データ
  • 特願2017-228394 (2017.11.28) JP
  • 特願2017-228396 (2017.11.28) JP
  • 特願2018-101753 (2018.5.28) JP
  • 特願2018-177416 (2018.9.21) JP
  • 2018JP43696 (2018.11.28) WO
発明の名称 (英語) NOVEL MICROALGAE AND USE FOR SAME NEW
発明の概要(英語) Provided is an alga belonging to Cyanidiophyceae having a diploid cell and a haploid cell form. Also provided is a nutrient composition containing an alga belonging to Cyanidiophyceae or an extract thereof.
(From EP3719114 A1)
従来技術、競合技術の概要(英語) BACKGROUND ART
Several species have been mass-cultured and used industrially as feeds, functional foods, cosmetic materials, and the like, in that microalgae have high ability to fix carbon dioxide compared to land plants, and in that they do not deal with agricultural products and growing sites.
When the microalgae are used industrially, it is preferable that the microalgae can be cultured outdoors in terms of cost. However, in order to be a microalgae that can be cultured in large quantities outdoors, conditions such as being resistant to environmental fluctuations (light, temperature, etc.), being able to be cultured under conditions in which other organisms cannot survive, being able to proliferate to a high density, etc. are required. Therefore, what has been industrially practical to date is limited to several species such as Chlorella, Euglena, Dunaliella, Spirulina, and the like.
The algae species is characterized in that the algae species can be cultured in an environment where other organisms such as high salt concentration, high pH, and low pH are difficult to grow. These algae species are rich in amino acids and vitamins and are used as raw materials for functional foods and supplements.
Meanwhile, algae belonging to Cyanidiophyceae, a monocytic primordial flushing, first proliferate in acidic sulfuric acid warm cloth. Cyanidium steels include the genera Cyanidioschyzon, Cyanidium, and Galdieria (Non-Patent Document 1), and monoploid are known only Cyanidioschyzon merolae (Non-Patent Document 2). Cyanidiosyzone merolae do not have a robust cell wall (Non-Patent Documents 1, 2).
Cyanidiosyzone merolae are constituted by a very simple set of organelles and the genomic sequence decryption is completed. Therefore, it has been used as a model organism for basic studies of photosynthetic organisms, and development of genetic modification techniques has also proceeded (Non-Patent Documents 3, 4).
特許請求の範囲(英語) [claim1]
1. An alga belonging to the genus Cyanidiophyceae, which has a diploid cell morphology and a monoploid cell morphology.

[claim2]
2. The bird of claim 1, wherein said monoploid cell morphology ruptures the cells under pH 7 conditions.

[claim3]
3. The alga according to claim 1 or 2, wherein the base sequence of the ribulose 1,5-bisphosphate carboxylase/oxygenase majority subunit gene has at least 90% identity to the base sequence set forth in SEQ ID no: 3 or 4.

[claim4]
4. The process of claim 3 wherein Cyanidium sp. YFU3 strain (FERM BP-22334), Cyanidium sp. An alga selected from the group consisting of HKN1 strain (FERM BP-22333), and mutants thereof.

[claim5]
5. The alga according to any one of claims 1 to 4, which is a transformant.

[claim6]
6. The bird of claim 5, wherein said transformant is fabricated by self-cloning.

[claim7]
7. The alga according to any one of claims 1 to 6, which is in the form of said monoploid cells.

[claim8]
8. (a) A method for producing a monoploid alga according to any one of claims 1 to 6, comprising the steps of: (a) culturing cells in the form of diploid cells, and (b) isolating cells in the form of monoploid cells arising during said culturing.

[claim9]
9. The method of claim 8, wherein the culturing of step (a) is conducted at a temperature of 30~ 50 °C, a pH of 1.0~ 5.0, and a co2 A concentration of the monoploid algae is 1 to 3%.

[claim10]
10. An algae culture comprising an algae according to any one of claims 1 to 7, wherein the proportion of the number of cells of the algae in the form of monoploid cells to the number of cells of the whole algae contained in the algae culture is 70~ 100%.

[claim11]
11. A low-temperature treated product obtained by subjecting the algae according to any one of claims 1 to 7 to a dry swelling treatment.

[claim12]
12. A nutritional ingredient composition comprising an alga belonging to the class Cyanidiophyceae or an extract thereof.

[claim13]
13. The nutritional ingredient composition of Claim 12 wherein said algae is a monoploid algae.

[claim14]
14. The nutritional component composition of Claims 12 or 13 wherein said algae are algae whose cells rupture under pH 7 conditions.

[claim15]
15. The nutritional component composition of any of claims 12 to 14 wherein said algae are algae belonging to the genus Cyanidioschyzon.

[claim16]
16. A nutritional component composition according to any of claims 12 to 14, wherein said algae are algae according to any of claims 1 to 7.

[claim17]
17. A nutritional ingredient composition according to any of claims 12 to 16, wherein said algae is a transformant in which the intracellular content of the at least one nutritional ingredient is increased.

[claim18]
18. The nutritional ingredient composition of Claim 17 wherein said transformant is prepared by self-cloning.

[claim19]
19. The nutritional ingredient composition according to any one of claims 12 to 18, which comprises at least one nutritional ingredient selected from the group consisting of amino acids, vitamins, proteins, lipids, and plant fibers.

[claim20]
20. A food product comprising a nutritional ingredient composition according to any of claims 12-19.
[claim21]
21. The food product of claim 20, wherein the food product is a functional food, or a nutritional supplement.

[claim22]
22. A feed or pet comprising a nutrient component composition according to any one of claims 12-19.

[claim23]
23. A cosmetic product comprising a nutrient component composition according to any one of claims 12-19.

[claim24]
24. (a) A method for producing a nutrient component, comprising the steps of: (a) disrupting cells of an alga belonging to the genus Cyanidiophyceae to obtain a disrupted cell product; and (b) separating at least one nutrient component from the disrupted cell product.

[claim25]
25. The method according to claim 24, wherein the nutrient component is at least one selected from the group consisting of amino acids, vitamins, proteins, lipids, and plant fibers.

[claim26]
26. The method of claim 25, wherein the amino acid is at least one selected from the group consisting of isoleucine, leucine, lysine, methionine, cystine, phenylalanine, tyrosine, threonine, tryptophan, valine, arginine, histidine, alanine, aspartic acid, glutamic acid, glycine, proline, serine, and γ-aminobutyric acid.

[claim27]
27. The composition of claim 25, wherein the vitamins are selected from the group consisting of vitamin a, β-carotene, vitamin b1, Vitamin b2, Vitamin b6, vitamin C, vitamin E, vitamin K1, Vitamin K2And at least one selected from the group consisting of niacin, inositol, folic acid, and biotin.

[claim28]
28. The method of any one of claims 24 to 27, wherein the algae are monoploid algae.

[claim29]
29. The method according to any one of claims 24 to 28, wherein said algae are algae in which the cells are ruptured under pH 7 conditions.

[claim30]
30. The method according to any one of claims 24 to 29, wherein the algae belong to the genus Cyanidioschyzon.

[claim31]
31. The method according to any one of claims 24 to 29, wherein the algae is an algae according to any one of claims 1 to 7.

[claim32]
32. The method according to any one of claims 24 to 30, wherein the algae is a transformant having an increased intracellular content of at least one nutrient component.

[claim33]
33. The method of claim 32, wherein the transformant is prepared by self-cloning.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MIYAGISHIMA Shinya
  • HIROOKA,Shunsuke
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms AREA
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