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Method for producing 4-aminocinnamic acid, and vector and host cell used in same

外国特許コード F210010508
整理番号 J1006-05WO
掲載日 2021年7月30日
出願国 中華人民共和国
出願番号 201980016395
公報番号 111801420
出願日 平成31年3月4日(2019.3.4)
公報発行日 令和2年10月20日(2020.10.20)
国際出願番号 JP2019008482
国際公開番号 WO2019168203
国際出願日 平成31年3月4日(2019.3.4)
国際公開日 令和元年9月6日(2019.9.6)
優先権データ
  • 特願2018-037794 (2018.3.2) JP
  • 2019JP08482 (2019.3.4) WO
発明の名称 (英語) Method for producing 4-aminocinnamic acid, and vector and host cell used in same
発明の概要(英語) Provided is a novel method for producing 4-aminocinnamic acid from 4-nitrophenylalanine. This method comprises: (1) converting 4-nitrophenylalanine into 4-nitrocinnamic acid; and (2) converting 4-nitrocinnamic acid into 4-aminocinnamic acid.
従来技術、競合技術の概要(英語) BACKGROUND ART
Documents of the prior art
Patent document
Patent document 1: international publication No. 2013/073519
Patent document 2: international publication No. 2015/141791
Patent document 3: international publication No. 2015/119251
Non-patent document
Non-patent document 1: suvannara et al, Macromolecules, (2014),47[5]1586-
特許請求の範囲(英語) [claim1]
1. A method of making 4-aminocinnamic acid from 4-nitrophenylalanine, the method comprising:
(1) converting 4-nitrophenylalanine into 4-nitrocinnamic acid;
(2) 4-nitro cinnamic acid is converted into 4-amino cinnamic acid.

[claim2]
2. The method according to claim 1, wherein the conversion of (1) is carried out using a first enzyme which comprises an amino acid sequence having a sequence homology of 80% or more with the amino acid sequence represented by SEQ ID NO. 1, 3 or 5 and has an activity of converting 4-nitrophenylalanine into 4-nitrocinnamic acid.

[claim3]
3. The method of claim 2, wherein the transformation of (1) is performed using a first host cell modified to express the first enzyme.

[claim4]
4. The method of claim 3, wherein the first host cell is a microorganism.

[claim5]
5. The method of claim 4, wherein the microorganism is a bacterium.

[claim6]
6. The method according to claim 5, wherein the transformation of (1) is carried out by a resting cell reaction using a resting cell of the bacterium as the first host cell.

[claim7]
7. The process according to any one of claims 1 to 6, wherein the conversion in (2) is carried out using a second enzyme which comprises an amino acid sequence having a sequence homology of 80% or more with the amino acid sequence represented by SEQ ID NO. 7, 9, 11, 13 or 15 and which has an activity of converting 4-nitrocinnamic acid into 4-aminocinnamic acid.

[claim8]
8. The method of claim 7, wherein the transforming of (2) is performed using a second host cell expressing the second enzyme.

[claim9]
9. The method of claim 8, wherein the second host cell is modified to express the second enzyme.

[claim10]
10. The method of claim 8 or 9, wherein the second host cell is a microorganism.

[claim11]
11. The method of claim 10, wherein the microorganism is a bacterium.

[claim12]
12. The method according to claim 11, wherein the transformation in (2) is performed by a resting cell reaction using a resting cell of the bacterium as the second host cell.

[claim13]
13. The method according to claim 6 or 12, wherein the resting cells are selected from the group consisting of cultured cells, powdered cells, and immobilized cells.

[claim14]
14. The process according to any one of claims 7 to 13, wherein the conversion of (2) is carried out at a pH of 8 to 9.

[claim15]
15. A method of making 4-aminocinnamic acid from glucose, the method comprising:
(a) producing phenylalanine from glucose;
(b) nitrating the phenylalanine obtained in (a) to convert it to 4-nitrophenylalanine;
(c) producing 4-aminocinnamic acid from 4-nitrophenylalanine obtained in (b) using the method of any one of claims 1 to 14.

[claim16]
16. A method of making 4-aminocinnamic acid from phenylalanine, said method comprising:
(b) nitrosation of phenylalanine to 4-nitrophenylalanine;
(c) producing 4-aminocinnamic acid from 4-nitrophenylalanine obtained in (b) using the method of any one of claims 1 to 14.

[claim17]
17. A method for producing 4-nitrocinnamic acid from 4-nitrophenylalanine, the method comprising using a first enzyme which comprises an amino acid sequence having a sequence homology of 80% or more with the amino acid sequence represented by SEQ ID NO. 1, 3 or 5 and has an activity to convert 4-nitrophenylalanine into 4-nitrocinnamic acid.

[claim18]
18. A method for producing 4-aminocinnamic acid from 4-nitrocinnamic acid, which comprises using a second enzyme which comprises an amino acid sequence having 80% or more sequence homology with the amino acid sequence represented by SEQ ID NO. 7, 9, 11, 13 or 15 and which has an activity of converting 4-nitrocinnamic acid into 4-aminocinnamic acid.

[claim19]
19. A vector carrying a nucleic acid encoding a first enzyme comprising an amino acid sequence having 80% or more sequence homology with the amino acid sequence represented by seq id No. 1, 3 or 5, and having an activity of converting 4-nitrophenylalanine into 4-nitrocinnamic acid.

[claim20]
20. A vector carrying a nucleic acid encoding a second enzyme comprising an amino acid sequence having 80% or more sequence homology with the amino acid sequence represented by SEQ ID NO. 7, 9, 11, 13 or 15, and having an activity of converting 4-nitrocinnamic acid into 4-aminocinnamic acid.

[claim21]
21. A host cell modified so as to express a first enzyme comprising an amino acid sequence having 80% or more sequence homology with the amino acid sequence represented by seq id No. 1, 3 or 5, and having an activity of converting 4-nitrophenylalanine into 4-nitrocinnamic acid.

[claim22]
22. A host cell modified so as to be capable of expressing a second enzyme comprising an amino acid sequence having 80% or more sequence homology with the amino acid sequence represented by seq id No. 7, 9, 11, 13 or 15, and having an activity of converting 4-nitrocinnamic acid into 4-aminocinnamic acid.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • TAKAYA NAOKI
  • MASUO SHUNSUKE
  • KAWASAKI YUKIE
  • MINAKAWA HAJIME
国際特許分類(IPC)
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