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METHOD FOR PRODUCING CAPPED RNA NEW

外国特許コード F210010583
整理番号 (J1036-03)
掲載日 2021年11月2日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2021JP006360
国際公開番号 WO 2021172204
国際出願日 令和3年2月19日(2021.2.19)
国際公開日 令和3年9月2日(2021.9.2)
優先権データ
  • 特願2020-032889 (2020.2.28) JP
発明の名称 (英語) METHOD FOR PRODUCING CAPPED RNA NEW
発明の概要(英語) A method for producing a capped RNA that is a 5' end cap-modified RNA, said method being characterized by comprising reacting an activated cap compound represented by formula (1) with a monophosphate RNA that is monophosphorylated at the 5' end. (In the formula, L represents a leaving group.) Preferably, the activated cap compound is a compound represented by formula (2).
従来技術、競合技術の概要(英語) BACKGROUND ART
In eukaryotic mRNA and the like, a 5 'cap structure in which 7-methylguanylate is 5'-5 'bonded to the 5' terminal via a triphosphate bond is known. A cap structure is known to facilitate translation of mRNA, and there is a demand for efficient introduction of a cap structure into mRNA in order to efficiently synthesize a protein of interest in a protein expression system or the like.
Synthesis of mRNA includes a chemical synthesis method based on an enzymatic transcription method and an amidite method, but the latter has a great advantage in that it can freely introduce) contributing to the improvement of the stability and translatability of the chemically modified (mRNA essential in mRNA medicine. On the other hand, there is no technique by which a cap structure can be easily introduced to the chemically synthesized mRNA. Only a technique is known in chemical synthesis in which the 5 'end is diphosphorylated, followed by enzymatically introducing a cap (e.g., NpL 1). Fig. 16 is a conceptual diagram illustrating this conventional cap introduction method. In this method, RNA diphosphorylated by transcriptional synthesis or chemical synthesis using an CAP enzyme is prepared, and a cap structure is further introduced to the 5 'end using the CAP enzyme.
In addition, another known method of CAP oxidation is a method in which guanine is methylated using an enzyme by solid-phase synthesis and monophosphate activation (for example, see Non-Patent Document 2). According to the method of this document, an imidazole group is introduced into the monophosphate group at the 5 'terminal of RNA, and a diphosphate group such as GDP (guanidinediphosphate) is reacted with the imidazole group to introduce a CAP structure at the 5' terminal of RNA.
Furthermore, another method is also known (see, for example, Non-Patent Document 3). This method also introduces a CAP structure to the 5 'terminal of RNA by introducing an imidazole group to the triphosphate group at the 5' terminal of RNA by solid phase synthesis, and reacting the imidazole group with a monophosphate group such as GMP (guanine phosphate).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • ABE Hiroshi
  • KIMURA Yasuaki
  • ABE Naoko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS IT JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN WS ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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