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METHOD FOR PRODUCTION OF DHA-CONTAINING PHOSPHOLIPID THROUGH MICROBIAL FERMENTATION meetings

Foreign code F110002383
Posted date Jan 20, 2011
Country WIPO
International application number 2008JP001394
International publication number WO 2008/149542
Date of international filing Jun 3, 2008
Date of international publication Dec 11, 2008
Priority data
  • P2007-148398 (Jun 4, 2007) JP
Title METHOD FOR PRODUCTION OF DHA-CONTAINING PHOSPHOLIPID THROUGH MICROBIAL FERMENTATION meetings
Abstract Disclosed is a method for producing a DHA phospholipid comprising an .omega.3 unsaturated fatty acid, particularly DHA, as a constituent lipid by using a microorganism in a simpler manner. Specifically disclosed is a method for producing a phospholipid comprising an .omega.3 unsaturated fatty acid as a constituent lipid, which comprises the steps of: growing a microorganism capable of producing the .omega.3 unsaturated fatty acid in a culture medium containing a carbon source; and further culturing the grown microorganism in a culture medium without any carbon source. The method enables to produce a highly value-added phospholipid which comprises an .omega.3 unsaturated fatty acid as a constituent lipid by using a microorganism capable of producing the .omega.3 unsaturated fatty acid in a large quantity.
Scope of claims (In Japanese)
【請求項1】炭素源を含む培地でω3系不飽和脂肪酸生産能を有する微生物を増殖させる工程、及び増殖させた前記微生物を、炭素源を含まない培地でさらに培養する工程を含む、ω3系不飽和脂肪酸を構成脂質とするリン脂質の製造方法。

【請求項2】ω3系不飽和脂肪酸生産能を有する微生物がラビリンチュラ類微生物又はトロウストチトリアレ類微生物である、請求項1に記載の製造方法

【請求項3】ラビリンチュラ類微生物がラビリンチュラ12B株である、請求項2に記載の製造方法。

【請求項4】ラビリンチュラ類微生物が、ラビリンチュラ属微生物、トラウストキトリウム属微生物、及びシゾキトリウム属微生物よりなる群から選ばれる、請求項2に記載の製造方法。

【請求項5】ラビリンチュラ類微生物がラビリンチュラ属S3-2株又はシゾキトリウム属SR21株である、請求項4に記載の製造方法。

【請求項6】ω3系不飽和脂肪酸がドコサヘキサエン酸である、請求項1~5の何れかに記載の製造方法。

【請求項7】強制通気を行いながら炭素源を含まない培地での培養を行う、請求項1~6の何れかに記載の製造方法。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
  • RESEARCH OF MICROBES CO., LTD.
  • Inventor
  • OKUYAMA, Hidetoshi
  • ORIKASA, Yoshitake
  • NISHIDA, Takanori
IPC(International Patent Classification)
Specified countries AE(UTILITY MODEL),AG,AL(UTILITY MODEL),AM(PROVISIONAL PATENT)(UTILITY MODEL),AO(UTILITY MODEL),AT(UTILITY MODEL),AU,AZ(UTILITY MODEL),BA,BB,BG(UTILITY MODEL),BH(UTILITY MODEL),BR(UTILITY MODEL),BW(UTILITY MODEL),BY(UTILITY MODEL),BZ(UTILITY MODEL),CA,CH,CN(UTILITY MODEL),CO(UTILITY MODEL),CR(UTILITY MODEL),CU(INVENTOR'S CERTIFICATE),CZ(UTILITY MODEL),DE(UTILITY MODEL),DK(UTILITY MODEL),DM(UTILITY MODEL),DO(UTILITY MODEL),DZ,EC(UTILITY MODEL),EE(UTILITY MODEL),EG(UTILITY MODEL),ES(UTILITY MODEL),FI(UTILITY MODEL),GB,GD,GE(UTILITY MODEL),GH(UTILITY CERTIFICATE),GM,GT,HN,HR(CONSENSUAL PATENT),HU(UTILITY MODEL),ID,IL,IN,IS,JP(UTILITY MODEL),KE(UTILITY MODEL),KG(UTILITY MODEL),KM,KN,KP(INVENTOR'S CERTIFICATE)(UTILITY MODEL),KR(UTILITY MODEL),KZ(PROVISIONAL PATENT)(UTILITY MODEL),LA,LC,LK,LR,LS(UTILITY MODEL),LT,LU,LY,MA,MD(UTILITY MODEL),ME(PETTY PATENT),MG,MK,MN,MW,MX(UTILITY MODEL),MY(UTILITY-INNOVATION),MZ(UTILITY MODEL),NA,NG,NI(UTILITY MODEL),NO,NZ,OM,PG,PH(UTILITY MODEL),PL(UTILITY MODEL),PT(UTILITY MODEL),RO,RS(PETTY PATENT),RU(UTILITY MODEL),SC,SD,SE,SG,SK(UTILITY MODEL),SL(UTILITY MODEL),SM,SV(UTILITY MODEL),SY,TJ(UTILITY MODEL),TM(PROVISIONAL PATENT),TN,TR(UTILITY MODEL),TT(UTILITY CERTIFICATE),TZ,UA(UTILITY MODEL),UG(UTILITY CERTIFICATE),US,UZ(UTILITY MODEL),VC(UTILITY CERTIFICATE),VN(PATENT FOR UTILITY SOLUTION),ZA,ZM,ZW,EP(AT,BE,BG,CH,CY,CZ,DE,DK,EE,ES,FI,FR,GB,GR,HR,HU,IE,IS,IT,LT,LU,LV,MC,MT,NL,NO,PL,PT,RO,SE,SI,SK,TR),OA(BF(UTILITY MODEL),BJ(UTILITY MODEL),CF(UTILITY MODEL),CG(UTILITY MODEL),CI(UTILITY MODEL),CM(UTILITY MODEL),GA(UTILITY MODEL),GN(UTILITY MODEL),GQ(UTILITY MODEL),GW(UTILITY MODEL),ML(UTILITY MODEL),MR(UTILITY MODEL),NE(UTILITY MODEL),SN(UTILITY MODEL),TD(UTILITY MODEL),TG(UTILITY MODEL)),AP(BW(UTILITY MODEL),GH(UTILITY MODEL),GM(UTILITY MODEL),KE(UTILITY MODEL),LS(UTILITY MODEL),MW(UTILITY MODEL),MZ(UTILITY MODEL),NA(UTILITY MODEL),SD(UTILITY MODEL),SL(UTILITY MODEL),SZ(UTILITY MODEL),TZ(UTILITY MODEL),UG(UTILITY MODEL),ZM(UTILITY MODEL),ZW(UTILITY MODEL)),EA(AM,AZ,BY,KG,KZ,MD,RU,TJ,TM)
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