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METHOD FOR PRODUCING PLANT WITH MODIFIED FLOWER MORPHOLOGY 新技術説明会

外国特許コード F110002730
整理番号 S2009-0356-N0
掲載日 2011年4月11日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2008JP063973
国際公開番号 WO 2009020101
国際出願日 平成20年8月4日(2008.8.4)
国際公開日 平成21年2月12日(2009.2.12)
優先権データ
  • 特願2007-204253 (2007.8.6) JP
発明の名称 (英語) METHOD FOR PRODUCING PLANT WITH MODIFIED FLOWER MORPHOLOGY 新技術説明会
発明の概要(英語) A plant with modified flower morphology is produced by suppressing the function of a transcription factor related to the polarity determination of the plant. More particularly, a plant with modified flower morphology is produced through the steps of: obtaining a transformed cell by introducing into a plant cell a chimeric DNA obtained by fusing a DNA encoding a transcription factor related to the polarity determination to a DNA encoding a functional peptide for converting a transcription factor into a transcription suppressor; and regenerating a transgenic plant from the transformed cell.
従来技術、競合技術の概要(英語) BACKGROUND ART
The view of the flower garden plants in the form of one of the important factor in the property. The occurrence of the flowers, inflorescence meristem tissue begins to differentiate from hanabara group, contained in a flower buds from meristem tissue hanabara, calyx pieces, petals, stamen, pistil 4 differentiated type of flower organs. Then, as a complex organ containing differentiated flower organs mature flower is formed. Altered flower plants that were in the form of, or creation of novel garden plants, having novel forms can be used in creation of the fruit and the like, the usability of the agriculturally.
Up to now, is a flower modifications of form, generally by multiplying plant varieties made by cross breeding techniques. However, in the conventional cross breeding techniques, plants that have the purpose to produce an embodiment, a long period of years and, since the skilled person's experience is necessary, simply and reliably in the form of a method of modifying the flower is demanded.
The occurrence of higher plants is generally in the form of a flower ABC and described by the model, this model MADS box belonging to the family A, B, through control of the transcription of the gene of class C flower is thought to be change in the mode of (Sakai one, flower morphogenesis of molecular genetic, to-date 'plant that determine the shape molecular mechanisms') 150-163 (2000) (Glycobiology). MADS box family gene, called a box storage stability MADS transcriptional factor containing the gene encoding region, composed of 30 or more of the genes forming the gene family. (AP1) For example APETALA 1, (AP2) such as class A APETALA gene, APETALA 3 (AP3), such as (PI) gene PISTILLATA class B, class C AGAMOUS (AG) of the transcription factor gene and the like, a variant of these changes in the form of a flower has been confirmed.
On the other hand, the relatively flat leaf has a basic structure in angiosperm tissue, in general, the proximal - distal, inner - outer, adaxial and abaxial --3 will be described with reference to the axis of the two terms can be. YABBY 1 As a transcription factor to determine the polarity of these (YAB1), YABBY 3 (YAB3) such as YABBY group, such as HD PHABULOSA KANADI (PHB) such as KANADI (KAN) group and group and the like, and participate in determining the polarity of the leaf lamina are known (non-patent document 1-3) respectively.
So far, genetic engineering methods to modify the form of a flower as the inventors of the present invention, any transcription factor transcriptional repressors found a method for converting are peptides (for example, Patent Document 1-7). This peptide, or a plant protein Class II ERF(Ethylene Responsive Element Binding Factor) (Zinc Finger Protein zinc-finger protein, for example Arabidopsis thaliana SUPERMAN protein or the like) from the cell from which the, has an extremely simple structure. Then, the present inventors, a variety of such transcription factor fuzed to the peptide and a gene encoding a fusion protein (chimeric protein) can be introduced into plants more, converted to a transcription factor and the transcriptional repressors, promotes transcription of the transcription factor and expression of a target gene is suppressed and a plant are produced successfully. Specifically, the above-described MADS box family AG Arabidopsis AP3 gene or gene expression of the gene, bind to the promoter region of each gene using suppression of a repressor, a method for the production of male sterility in the form of the method of modifying the flower or the established (Patent Document 8-9). However, the leaf of a plant transcription factor involved in determining the polarity of the (YAB1, KAN, PHB) transcriptional repressors to recombinant converted by overexpressing, the polarity of the transcription factor involved in plant organ determined by inhibiting the function of the form of flowers to be modified is not known.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • UNIVERSITY OF TSUKUBA
  • NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
  • HOKKO CHEMICAL INDUSTRY CO., LTD.
  • 発明者(英語)
  • ONO, Michiyuki
  • TERAKAWA, Teruhiko
  • TAKAGI, Masaru
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MT NL NO PL PT RO SE SI SK TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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