Top > Search of International Patents > METHOD FOR PRODUCING PLANT WITH MODIFIED FLOWER MORPHOLOGY

METHOD FOR PRODUCING PLANT WITH MODIFIED FLOWER MORPHOLOGY meetings

Foreign code F110002730
File No. S2009-0356-N0
Posted date Apr 11, 2011
Country WIPO
International application number 2008JP063973
International publication number WO 2009020101
Date of international filing Aug 4, 2008
Date of international publication Feb 12, 2009
Priority data
  • P2007-204253 (Aug 6, 2007) JP
Title METHOD FOR PRODUCING PLANT WITH MODIFIED FLOWER MORPHOLOGY meetings
Abstract A plant with modified flower morphology is produced by suppressing the function of a transcription factor related to the polarity determination of the plant. More particularly, a plant with modified flower morphology is produced through the steps of: obtaining a transformed cell by introducing into a plant cell a chimeric DNA obtained by fusing a DNA encoding a transcription factor related to the polarity determination to a DNA encoding a functional peptide for converting a transcription factor into a transcription suppressor; and regenerating a transgenic plant from the transformed cell.
Outline of related art and contending technology BACKGROUND ART
The view of the flower garden plants in the form of one of the important factor in the property. The occurrence of the flowers, inflorescence meristem tissue begins to differentiate from hanabara group, contained in a flower buds from meristem tissue hanabara, calyx pieces, petals, stamen, pistil 4 differentiated type of flower organs. Then, as a complex organ containing differentiated flower organs mature flower is formed. Altered flower plants that were in the form of, or creation of novel garden plants, having novel forms can be used in creation of the fruit and the like, the usability of the agriculturally.
Up to now, is a flower modifications of form, generally by multiplying plant varieties made by cross breeding techniques. However, in the conventional cross breeding techniques, plants that have the purpose to produce an embodiment, a long period of years and, since the skilled person's experience is necessary, simply and reliably in the form of a method of modifying the flower is demanded.
The occurrence of higher plants is generally in the form of a flower ABC and described by the model, this model MADS box belonging to the family A, B, through control of the transcription of the gene of class C flower is thought to be change in the mode of (Sakai one, flower morphogenesis of molecular genetic, to-date 'plant that determine the shape molecular mechanisms') 150-163 (2000) (Glycobiology). MADS box family gene, called a box storage stability MADS transcriptional factor containing the gene encoding region, composed of 30 or more of the genes forming the gene family. (AP1) For example APETALA 1, (AP2) such as class A APETALA gene, APETALA 3 (AP3), such as (PI) gene PISTILLATA class B, class C AGAMOUS (AG) of the transcription factor gene and the like, a variant of these changes in the form of a flower has been confirmed.
On the other hand, the relatively flat leaf has a basic structure in angiosperm tissue, in general, the proximal - distal, inner - outer, adaxial and abaxial --3 will be described with reference to the axis of the two terms can be. YABBY 1 As a transcription factor to determine the polarity of these (YAB1), YABBY 3 (YAB3) such as YABBY group, such as HD PHABULOSA KANADI (PHB) such as KANADI (KAN) group and group and the like, and participate in determining the polarity of the leaf lamina are known (non-patent document 1-3) respectively.
So far, genetic engineering methods to modify the form of a flower as the inventors of the present invention, any transcription factor transcriptional repressors found a method for converting are peptides (for example, Patent Document 1-7). This peptide, or a plant protein Class II ERF(Ethylene Responsive Element Binding Factor) (Zinc Finger Protein zinc-finger protein, for example Arabidopsis thaliana SUPERMAN protein or the like) from the cell from which the, has an extremely simple structure. Then, the present inventors, a variety of such transcription factor fuzed to the peptide and a gene encoding a fusion protein (chimeric protein) can be introduced into plants more, converted to a transcription factor and the transcriptional repressors, promotes transcription of the transcription factor and expression of a target gene is suppressed and a plant are produced successfully. Specifically, the above-described MADS box family AG Arabidopsis AP3 gene or gene expression of the gene, bind to the promoter region of each gene using suppression of a repressor, a method for the production of male sterility in the form of the method of modifying the flower or the established (Patent Document 8-9). However, the leaf of a plant transcription factor involved in determining the polarity of the (YAB1, KAN, PHB) transcriptional repressors to recombinant converted by overexpressing, the polarity of the transcription factor involved in plant organ determined by inhibiting the function of the form of flowers to be modified is not known.
Scope of claims (In Japanese)請求の範囲 [1]
植物の極性決定に関与する転写因子の機能を抑制することにより、植物体の花の形態を改変させることを特徴とする花の形態が改変された植物体の生産方法。

[2]
前記転写因子をコードするDNAと転写因子を転写抑制因子に転換する機能性ペプチドをコードするDNAとを融合させたキメラDNAを植物細胞に導入して形質転換細胞を得る工程と、前記形質転換細胞から形質転換植物体を再生させる工程を含む、請求項1に記載の花の形態が改変された植物体の生産方法。

[3]
前記転写因子をコードするDNAが、
(1)配列番号2に記載のアミノ酸配列をコードするDNA
(2)配列番号1に記載の塩基配列を含むDNA
(3)配列番号2に記載のアミノ酸配列において1若しくは数個のアミノ酸が置換、欠失、挿入及び/又は付加したアミノ酸配列をコードするDNA
(4)配列番号1に記載の塩基配列と相補的な塩基配列からなるDNAにストリンジェントな条件下でハイブリダイズするDNA
(5)配列番号2に記載のアミノ酸配列と90%以上の同一性を有するアミノ酸配列をコードするDNAから選択されることを特徴とする、請求項2に記載の花の形態が改変された植物体の生産方法。

[4]
前記転写因子をコードするDNAが、
(1)配列番号4に記載のアミノ酸配列をコードするDNA
(2)配列番号3に記載の塩基配列を含むDNA
(3)配列番号4に記載のアミノ酸配列において1若しくは数個のアミノ酸が置換、欠失、挿入及び/又は付加したアミノ酸配列をコードするDNA
(4)配列番号3に記載の塩基配列と相補的な塩基配列からなるDNAにストリンジェントな条件下でハイブリダイズするDNA
(5)配列番号4に記載のアミノ酸配列と90%以上の同一性を有するアミノ酸配列をコードするDNAから選択されることを特徴とする、請求項2に記載の花の形態が改変された植物体の生産方法。

[5]
前記転写因子をコードするDNAが、
(1)配列番号6に記載のアミノ酸配列をコードするDNA
(2)配列番号5に記載の塩基配列を含むDNA
(3)配列番号6に記載のアミノ酸配列において1若しくは数個のアミノ酸が置換、欠失、挿入及び/又は付加したアミノ酸配列をコードするDNA
(4)配列番号5に記載の塩基配列と相補的な塩基配列からなるDNAにストリンジェントな条件下でハイブリダイズするDNA
(5)配列番号6に記載のアミノ酸配列と90%以上の同一性を有するアミノ酸配列をコードするDNAから選択されることを特徴とする、請求項2に記載の花の形態が改変された植物体の生産方法。

[6]
前記機能性ペプチドが、配列番号7から配列番号46のいずれかに示されるアミノ酸配列を有するペプチドであることを特徴とする、請求項2~5のいずれか一項に記載の花の形態が改変された植物体の生産方法。

[7]
請求項1~6のいずれか1項に記載の生産方法により生産された植物体。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF TSUKUBA
  • NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
  • HOKKO CHEMICAL INDUSTRY CO., LTD.
  • Inventor
  • ONO, Michiyuki
  • TERAKAWA, Teruhiko
  • TAKAGI, Masaru
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MT NL NO PL PT RO SE SI SK TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close