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Method for preparation of cartilage cell

Foreign code F110003030
Posted date May 13, 2011
Country United States of America
Application number 52435908
Gazette No. 20090324560
Gazette No. 9951312
Date of filing Jan 23, 2008
Gazette Date Dec 31, 2009
Gazette Date Apr 24, 2018
International application number JP2008051327
International publication number WO2008091013
Date of international filing Jan 23, 2008
Date of international publication Jul 31, 2008
Priority data
  • P2007-012160 (Jan 23, 2007) JP
  • 2008WO-JP51327 (Jan 23, 2008) WO
Title Method for preparation of cartilage cell
Abstract (US9951312)
The present invention provides a cell derived from a perichondrial tissue, the cell being capable of differentiating into a chondrocyte.
The present invention also provides a method of preparing the above-described cell and a composition comprising the same.
A method of preparing a chondrocyte and a medium for use in the method are also provided.
Scope of claims [claim1]
1. A method of preparing chondrocytes, comprising: separating an outermost layer and a fibroblast layer of human perichondrium away from a perichondrium-cartilage transition zone of the human perichondrium to produce a tissue section from the outermost layer and the fibroblast layer without the perichondrium-cartilage transition zone,
then treating the tissue section from the outermost layer and the fibroblast layer with collagenase to produce an isolated perichondrocyte population, and
then differentiating the perichondrocytes from the isolated perichondrocyte population from the tissue section from the outermost layer and the fibroblast layer into chondrocytes in a medium containing a serum and further containing an insulin-like growth factor and/or a basic fibroblast growth factor.
[claim2]
2. The method according to claim 1, wherein the serum is bovine serum.
[claim3]
3. The method according to claim 1, wherein the serum is an autoserum.
[claim4]
4. The method according to claim 1, wherein the isolated perichondrocyte population is differentiated into chondrocytes in the medium further containing DMEM/F 12, antibiotics and antimycotics.
[claim5]
5. The method according to claim 4, wherein the medium further contains dexamethasone and/or L-ascorbic acid.
[claim6]
6. A method of transplanting chondrocytes: comprising: preparing the chondrocytes according to the method of claim 1; and
transplanting the chondrocytes and/or a cartilage tissue formed by said chondrocytes into a living body.
[claim7]
7. A method of preparing a matrix produced by chondrocytes, comprising preparing the chondrocytes according to the method of claim 1; and
allowing said chondrocytes to produce the matrix.
[claim8]
8. The method according to claim 7, wherein the matrix is type II collagen and/or proteoglycan.
[claim9]
9. The method according to claim 1, wherein the medium contains the insulin-like growth factor.
[claim10]
10. The method according to claim 1, wherein the method further comprises proliferating the differentiated cell population.
[claim11]
11. A method of transplanting a cell derived from a human perichondrial tissue, comprising: separating an outermost layer and a fibroblast layer of human perichondrium away from a perichondrium-cartilage transition zone of the human perichondrium to produce a tissue section from the outermost layer and the fibroblast layer without the perichondrium-cartilage transition zone,
then treating the tissue section from the outermost layer and the fibroblast layer with collagenase to produce an isolated perichondrocyte population;
then culturing the isolated perichondrocyte population in a centrifuge tube;
then proliferating and differentiating the perichondrocytes from the isolated perichondrocyte population into chondrocytes in a medium containing a serum and further containing an insulin-like growth factor and/or a basic fibroblast growth factor; and
then transplanting the chondrocytes or a cartilage tissue formed by the chondrocytes into a subject.
[claim12]
12. The method of transplanting a cell according to claim 11, wherein differentiating the cell into chondrocytes in the presence of the insulin-like growth factor and/or the basic fibroblast growth factor is accompanied by allowing said chondrocytes to produce a matrix.
[claim13]
13. The method according to claim 11, wherein the serum is bovine serum.
[claim14]
14. The method according to claim 11, wherein the serum is an autoserum.
[claim15]
15. The method according to claim 11, wherein the medium further contains DMEM/F 12, a serum, antibiotics, and antimycotics.
[claim16]
16. The method according to claim 11, wherein the medium further contains dexamethasone and/or L-ascorbic acid.
[claim17]
17. The method according to claim 11, wherein the medium contains the insulin-like growth factor.
[claim18]
18. The method according to claim 11, wherein the method comprises maintaining chondrogenic phenotype of the chondrocytes or the cartilage tissue formed by the chondrocytes for a month after the transplanting.
[claim19]
19. The method according to claim 11, wherein the method comprises maintaining cell count of the chondrocytes or the cartilage tissue formed by the chondrocytes for a month after the transplanting.
  • Inventor, and Inventor/Applicant
  • TANIGUCHI HIDEKI
  • KOBAYASHI SHINJI
  • YOKOHAMA CITY UNIVERSITY
IPC(International Patent Classification)
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