Top > Search of International Patents > Method for preparation of cartilage cell

Method for preparation of cartilage cell

Foreign code F110003033
Posted date May 13, 2011
Country EPO
Application number 08704109
Gazette No. 2119767
Gazette No. 2119767
Date of filing Jan 23, 2008
Gazette Date Nov 18, 2009
Gazette Date Jan 20, 2016
International application number JP2008051327
International publication number WO2008091013
Date of international filing Jan 23, 2008
Date of international publication Jul 31, 2008
Priority data
  • 2008JP051327 (Jan 23, 2008) WO
  • P2007-012160 (Jan 23, 2007) JP
Title Method for preparation of cartilage cell
Abstract The present invention provides a cell derived from a perichondrial tissue, the cell being capable of differentiating into a chondrocyte.
The present invention also provides a method of preparing the above-described cell and a composition comprising the same.
A method of preparing a chondrocyte and a medium for use in the method are also provided.
Scope of claims [claim1]
1. A cell derived from a human perichondrial tissue wherein Alcian blue does not stain said perichondrial tissue, said cell being capable of differentiating into a chondrocyte.
[claim2]
2. A method of preparing the cell according to claim 1, wherein the method comprises culturing cells isolated from a human perichondrial tissue, wherein Alcian blue does not stain said perichondrial tissue.
[claim3]
3. A composition comprising the cell according to claim 1.
[claim4]
4. The composition according to claim 3, which further comprises: (a) a matrix produced by the cell according to claim 1; and/or (b) scaffold.
[claim5]
5. The composition according to claim 3 or 4 which is suitable for use in proliferating a cell derived from a human perichondrial tissue, said cell being capable of differentiating into a chondrocyte.
[claim6]
6. The composition according to claim 3 or 4 for use in: (a) cell transplant; or (b) cell transplant, wherein the cell transplant aims at any one of treatment of congenital auricular deformity, treatment of costicartilage defect, treatment of damage to articular cartilage, treatment of tracheal cartilage defect, rhinoplasty, genioplasty, plastic surgery of small facial recesses, corrective surgery of facial left-right asymmetry, corrective surgery around eyelids, or cosmetic surgery of face.
[claim7]
7. A method of preparing chondrocytes, comprising differentiating the cell according to claim 1 into chondrocytes.
[claim8]
8. The method according to claim 7, wherein (a) cell masses are formed by culturing the cell according to claim 1 in centrifuge tubes; or (b) the cell according to claim 1 is multi-layered by plate culture.
[claim9]
9. The method according to claim 7 or 8, wherein the cell according to claim 1 is proliferated and/or differentiated in a medium containing a serum.
[claim10]
10. The method according to claim 9, wherein the serum is bovine serum or an autoserum.
[claim11]
11. The method according to any one of claims 7 to 10, wherein the cell according to claim 1 is differentiated into chondrocytes in a medium containing DMEM/F12, a serum, antibiotics and antimycotics.
[claim12]
12. The method according to claim 11, wherein the medium further contains dexamethasone and/or L-ascorbic acid.
[claim13]
13. The method according to claim 11 or 12, wherein the medium further contains an insulin-like growth factor and/or a basic fibroblast growth factor.
[claim14]
14. A method of preparing a matrix produced by chondrocytes, comprising differentiating the cell according to claim 1 into chondrocytes and allowing said chondrocytes to produce the matrix.
[claim15]
15. The method according to claim 14, wherein the matrix is type II collagen and/or proteoglycan.
  • Applicant
  • YOKOHAMA CITY UNIVERSITY
  • Inventor
  • TANIGUCHI HIDEKI
  • KOBAYASHI SHINJI
IPC(International Patent Classification)
Specified countries Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close