TOP > 外国特許検索 > Reagent for introduction of protein or gene

Reagent for introduction of protein or gene UPDATE

外国特許コード F110003065
整理番号 134-US
掲載日 2011年5月25日
出願国 アメリカ合衆国
出願番号 31264807
公報番号 20100291672
公報番号 8338643
出願日 平成19年11月26日(2007.11.26)
公報発行日 平成22年11月18日(2010.11.18)
公報発行日 平成24年12月25日(2012.12.25)
国際出願番号 JP2007073266
国際公開番号 WO2008062911
国際出願日 平成19年11月26日(2007.11.26)
国際公開日 平成20年5月29日(2008.5.29)
優先権データ
  • 特願2006-317841 (2006.11.24) JP
  • 2007WO-JP73266 (2007.11.26) WO
発明の名称 (英語) Reagent for introduction of protein or gene UPDATE
発明の概要(英語) (US8338643)
The present invention provides a reagent for introducing a protein or gene into a cell.
The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (wherein in formula (I)-1: L is a single bond, —CONH—, or —S—S—; M1 is —(CH2)k— or —(CH2CH2O)k— (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).
特許請求の範囲(英語) [claim1]
1. A reagent for introducing a protein into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1:

wherein: L is a single bond, -- CONH -- , or -- S -- S -- ; M1 is -- (CH2)k -- or -- (CH2CH2O)k -- ; k is an integer between 1 and 11; and m1 and m2 are each independently an integer between 11 and 21.
[claim2]
2. A reagent for introducing a gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1:

wherein: L is a single bond, -- CONH -- , or -- S -- S -- ; M1 is -- (CH2)k -- or -- (CH2CH2O)k -- ; k is an integer between 1 and 11; and m1 and m2 are each independently an integer between 11 and 21; with the proviso that both m1 and m2 are not an integer of 15.
[claim3]
3. The reagent according to claim 1 or 2, wherein the composition further comprises at least one substance selected from the group consisting of diacylphosphatidylcholine, cholesterol, and an amphipathic molecule to which a polyethylene glycol chain is bound.
[claim4]
4. The reagent according to claim 3, wherein one or two acyl chains of diacylphosphatidylcholine is an oleoyl group.
[claim5]
5. The reagent according to claim 1 or 2, wherein the composition is a molecular assembly dispersed in an aqueous medium.
[claim6]
6. The reagent according to claim 5, wherein the molecular assembly forms a bilayer membrane vesicle structure, and wherein an inner aqueous phase thereof includes a protein or gene.
[claim7]
7. The reagent according to claim 1 or 2, wherein the composition is a dry powder product.
[claim8]
8. The reagent according to claim 7, wherein the dry powder product can form a bilayer membrane vesicle structure including a protein or gene when dispersed in an aqueous medium.
[claim9]
9. The reagent according to claim 1 or 2, wherein the cell is: a cell constituting a part of body tissue; plasma, serum or blood; or a cell cultured in a medium containing a part of components thereof.
[claim10]
10. A kit for introducing a protein or gene into a cell, which comprises the reagent according to claim 1 or 2.
[claim11]
11. A cationic amino acid type lipid represented by the following formula (I)-3:

wherein: M2 is -- (CH2)k' -- or -- (CH2CH2O)k' -- ; k' is an integer between 1 and 14; and p1 and p2 are each independently an integer between 11 and 21.
[claim12]
12. A reagent for introducing a protein into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1:

wherein: L is -- CONH -- , or -- S -- S -- ; M1 is -- (CH2)k -- or -- (CH2CH2O)k -- ; k is an integer between 2 and 14; and m1 and m2 are each independently an integer between 11 and 21.
[claim13]
13. A reagent for introducing a gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1:

wherein: L is -- CONH -- , or -- S -- S -- ; M1 is -- (CH2)k -- or -- (CH2CH2O)k -- ; k is an integer between 2 and 14; and m1 and m2 are each independently an integer between 11 and 21; with the proviso that both m1 and m2 are not an integer of 15.
[claim14]
14. The reagent according to claim 12 or 13, wherein the composition further comprises at least one substance selected from the group consisting of diacylphosphatidylcholine, cholesterol, and an amphipathic molecule to which a polyethylene glycol chain is bound.
[claim15]
15. The reagent according to claim 14, wherein one or two acyl chains of diacylphosphatidylcholine is an oleoyl group.
[claim16]
16. The reagent according to claim 12 or 13, wherein the composition is a molecular assembly dispersed in an aqueous medium.
[claim17]
17. The reagent according to claim 16, wherein the molecular assembly forms a bilayer membrane vesicle structure, and wherein an inner aqueous phase thereof includes a protein or gene.
[claim18]
18. The reagent according to claim 12 or 13, wherein the composition is a dry powder product.
[claim19]
19. The reagent according to claim 18, wherein the dry powder product can form a bilayer membrane vesicle structure including a protein or gene when dispersed in an aqueous medium.
[claim20]
20. The reagent according to claim 12 or 13, wherein the cell is: a cell constituting a part of body tissue; plasma, serum or blood; or a cell cultured in a medium containing a part of components thereof.
[claim21]
21. A kit for introducing a protein or gene into a cell, which comprises the reagent according to claim 12 or 13.
[claim22]
22. The reagent according to claim 1, wherein k is an integer between 3 and 5.
[claim23]
23. The reagent according to claim 2, wherein k is an integer between 3 and 5.
  • 発明者/出願人(英語)
  • TAKEOKA SHINJI
  • TAKEDA NAOYA
  • KURUMIZAKA HITOSHI
  • SAKANE ISAO
  • IKEGAYA NAMIKO
  • OBATA YOSUKE
  • SAITO SYUNSUKE
  • WASEDA UNIVERSITY
国際特許分類(IPC)
米国特許分類/主・副
  • C07C237/08
  • C07C237/12
  • C07C237/22
  • C07K005/02D
  • C07K005/06B
  • C07K005/08B
技術導入、技術提携、実用化開発(受託研究・共同研究等)のご相談を承っております。お気軽にご連絡ください。

PAGE TOP

close
close
close
close
close
close