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Method for production of DHA-containing phospholipid through microbial fermentation meetings

Foreign code F110003071
Posted date May 25, 2011
Country United States of America
Application number 45186008
Gazette No. 20100105113
Gazette No. 8652814
Date of filing Jun 3, 2008
Gazette Date Apr 29, 2010
Gazette Date Feb 18, 2014
International application number JP2008001394
International publication number WO2008149542
Date of international filing Jun 3, 2008
Date of international publication Dec 11, 2008
Priority data
  • P2007-148398 (Jun 4, 2007) JP
  • 2008WO-JP01394 (Jun 3, 2008) WO
Title Method for production of DHA-containing phospholipid through microbial fermentation meetings
Abstract (US8652814)
Disclosed is a method for producing a DHA phospholipid comprising an omega3 unsaturated fatty acid, particularly DHA, as a constituent lipid by using a microorganism in a simpler manner.
Specifically disclosed is a method for producing a phospholipid comprising an omega3 unsaturated fatty acid as a constituent lipid, which comprises the steps of: growing a microorganism capable of producing the omega3 unsaturated fatty acid in a culture medium containing a carbon source; and further culturing the grown microorganism in a culture medium without any carbon source.
The method enables to produce a highly value-added phospholipid which comprises an omega3 unsaturated fatty acid as a constituent lipid by using a microorganism capable of producing the omega3 unsaturated fatty acid in a large quantity.
Scope of claims [claim1]
1. A method for increasing the content of a phospholipid comprising an omega 3 unsaturated fatty acid as a constituent lipid in a microorganism capable of producing the omega 3 unsaturated fatty acid, comprising the steps of: (a) growing the microorganism in a culture medium containing a carbon source, whereby the phospholipid is produced at a first level and stored in a microbial cell body of the microorganism; and
(b) further growing the microorganism from step (a) in a culture medium without any carbon source, whereby the content of the phospholipid in the microorganism is increased to a second level that is greater than the first level.
[claim2]
2. The method as set forth in claim 1, wherein the microorganism is a labyrinthulean microorganism or thraustochytride microorganism.
[claim3]
3. The method as set forth in claim 2, wherein the labyrinthulean microorganism is labyrinthulean strain 12B.
[claim4]
4. The method as set forth in claim 2, wherein the labyrinthulean microorganism is selected from the group consisting of genus Labyrinthula microorganisms, genus Thraustochytrium microorganisms and genus Schizochytrium microorganisms.
[claim5]
5. The method as set forth in claim 1, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim6]
6. The method as set forth in claim 2, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim7]
7. The method as set forth in claim 3, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim8]
8. The method as set forth in claim 4, wherein the omega 3 unsaturated fatty acid is docosahexaenoic acid.
[claim9]
9. The method as set forth in claim 1, wherein step (b) is carried out under forced aeration.
[claim10]
10. The method as set forth in claim 2, wherein step (b) is carried out under forced aeration.
[claim11]
11. The method as set forth in claim 3, wherein step (b) is carried out under forced aeration.
[claim12]
12. The method as set forth in claim 4, wherein step (b) is carried out under forced aeration.
[claim13]
13. The method as set forth in claim 5, wherein step (b) is carried out under forced aeration.
[claim14]
14. A method for increasing the content of a phospholipid comprising a docosahexaenoic acid as a constituent lipid in labyrinthulean strain 12B, comprising the steps of: (a) growing the labyrinthulean strain 12B in a culture medium containing a carbon source, whereby the phospholipid is produced at a first level and stored in a microbial cell body of the microorganism; and
(b) further growing the labyrinthulean strain 12B from step (a) in a culture medium without any carbon source, whereby the content of the phospholipid in the microorganism is increased to a second level that is greater than the first level.
[claim15]
15. The method as set forth in claim 14, wherein the microorganism is grown under forced aeration.
  • Inventor, and Inventor/Applicant
  • OKUYAMA HIDETOSHI
  • ORIKASA YOSHITAKE
  • NISHIDA TAKANORI
  • HOKKAIDO UNIVERSITY
  • RESEARCH OF MICROBES
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • C12N001/10
  • C12P007/64E2H
  • C12R001/90
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