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Reagent for introduction of protein or gene

Foreign code F110003080
File No. 134-EP
Posted date Jun 6, 2011
Country EPO
Application number 07849971
Gazette No. 2116599
Gazette No. 2116599
Date of filing Nov 26, 2007
Gazette Date Nov 11, 2009
Gazette Date Aug 6, 2014
International application number JP2007073266
International publication number WO2008062911
Date of international filing Nov 26, 2007
Date of international publication May 29, 2008
Priority data
  • 2007JP073266 (Nov 26, 2007) WO
  • P2006-317841 (Nov 24, 2006) JP
Title Reagent for introduction of protein or gene
Abstract The present invention provides a reagent for introducing a protein or gene into a cell.
The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (see diagramm) (wherein in formula (I)-1: L is a single bond, -CONH-, or -S-S-; M **1 is -(CH 2) k- or -(CH 2CH 2O) k- (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).
Scope of claims [claim1]
1. Use of a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1, (II)-1 or (III)-1: (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; or (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21,
for introducing a protein into a cell.
[claim2]
2. Use of a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1, (II)-1 or (III)-1: (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21 with the proviso that the case where both m1 and m2 are 15 is excluded; (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21; or (see diagramm) wherein: L is -CONH-, or -S-S-; M **1 is -(CH 2) k- and k is an integer between 0 and 7; and m1 and m2 are each independently an integer between 11 and 21,
for introducing a gene into a cell.
[claim3]
3. The use according to claim 1 or 2, wherein the composition further comprises at least one substance selected from the group consisting of diacylphosphatidylcholine, cholesterol, and an amphipathic molecule to which a polyethylene glycol chain is bound.
[claim4]
4. The use according to claim 3, wherein one or two acyl chains of diacylphosphatidylcholine is an oleoyl group.
[claim5]
5. The use according to claim 1 or 2, wherein the composition is a molecular assembly dispersed in an aqueous medium.
[claim6]
6. The use according to claim 5, wherein the molecular assembly forms a bilayer membrane vesicle structure, and wherein an inner aqueous phase thereof includes a protein or gene.
[claim7]
7. The use according to claim 1 or 2, wherein the composition is a dry powder product.
[claim8]
8. The use according to claim 7, wherein the dry powder product can form a bilayer membrane vesicle structure including a protein or gene when dispersed in an aqueous medium.
[claim9]
9. The use according to claim 1 or 2, wherein the cell is: a cell constituting a part of body tissue; plasma, serum or blood; or a cell cultured in a medium containing a part of components thereof.
[claim10]
10. A kit for introducing a protein or gene into a cell, which comprises the composition as defined in any one of claims 1 to 9.
[claim11]
11. A cationic amino acid type lipid represented by the following formula (I)-3, (II)-3 or (III)-3: (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21; (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21; or (see diagramm) wherein: M **2 is -(CH 2) k'- and k' is an integer between 0 and 7; and p1 and p2 are each independently an integer between 11 and 21.
  • Applicant
  • WASEDA UNIVERSITY
  • Inventor
  • TAKEOKA SHINJI
  • TAKEDA NAOYA
  • KURUMIZAKA HITOSHI
  • SAKANE ISAO
  • IKEGAYA NAMIKO
  • OBATA YOSUKE
  • SAITO SYUNSUKE
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • C07C237/08
  • C07C237/12
  • C07C237/22
  • C07K005/02D
  • C07K005/06B
  • C07K005/08B
Specified countries Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
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