TOP > 外国特許検索 > Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same

Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same UPDATE

外国特許コード F110003168
整理番号 A141-05WO
掲載日 2011年6月17日
出願国 アメリカ合衆国
出願番号 59946005
公報番号 20070050858
公報番号 9347936
出願日 平成17年3月24日(2005.3.24)
公報発行日 平成19年3月1日(2007.3.1)
公報発行日 平成28年5月24日(2016.5.24)
国際出願番号 JP2005005350
国際公開番号 WO2005093051
国際出願日 平成17年3月24日(2005.3.24)
国際公開日 平成17年10月6日(2005.10.6)
優先権データ
  • 特願2004-096995 (2004.3.29) JP
  • 特願2004-380376 (2004.12.28) JP
  • 2005WO-JP05350 (2005.3.24) WO
発明の名称 (英語) Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same UPDATE
発明の概要(英語) (US9347936)
The present invention provides an inactive Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) knockin animal in which only the protein kinase activity of CaMKIIα has been specifically impaired.
Since CaMKIIα is considered to be involved in higher brain functions including learning and memory, and inhibit epileptic seizure and brain disorders caused by ischemia, the inactive CaMKIIα knockin animal of the present invention is widely usable for various studies of the brain and nerve, such as the studies of mechanisms of learning disability, dysmnesia, epileptic seizure and brain disorders.
特許請求の範囲(英語) [claim1]
1. An inactive Ca2+/calmodulin-dependent protein kinase IIalpha (CaMKIIalpha ) knockin nonhuman animal, wherein a CaMKIIalpha gene of one or both of homologous chromosomes is substituted into an inactive type so that an inactive CaMKIIalpha is expressed, wherein lysine corresponding to Lys-42 in the catalytic domain of mouse CaMKIIalpha is substituted by arginine; and thereby a protein kinase activity of CaMKIIalpha is specifically impaired while a calmodulin binding capacity of CaMKIIalpha and a capacity of multimerizing subunits are maintained, wherein the nucleus accumbens of the brain of the inactive CaMKIIalpha knockin nonhuman animal has lower neuronal activity as compared to that of a wild-type, while there is no substantial difference in neuronal activities in the cerebral cortex and caudate-putamen as compared to those of the wild-type,
and wherein the inactive CaMKIIalpha knockin nonhuman animal is produced by a gene targeting method.
[claim2]
2. The inactive CaMKIIalpha knockin nonhuman animal of claim 1, wherein the inactive CaMKIIalpha knockin nonhuman animal is a rodent animal.
[claim3]
3. The inactive CaMKIIalpha knockin nonhuman animal of claim 2, wherein the inactive CaMKIIalpha knockin nonhuman animal is a mouse.
  • 発明者/出願人(英語)
  • YAMAGATA YOKO
  • YANAGAWA YUCHIO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • A01K067/027M2
  • C12N009/12C
  • C12N015/85A
  • G01N033/50D2J4
  • K01K217/07A
  • K01K267/03
参考情報 (研究プロジェクト等) CREST Understanding the Brain AREA
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