Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same
|Posted date||Jun 17, 2011|
|Country||United States of America|
|Date of filing||Mar 24, 2005|
|Gazette Date||Mar 1, 2007|
|Gazette Date||May 24, 2016|
|International application number||JP2005005350|
|International publication number||WO2005093051|
|Date of international filing||Mar 24, 2005|
|Date of international publication||Oct 6, 2005|
|Title||Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same|
The present invention provides an inactive Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) knockin animal in which only the protein kinase activity of CaMKIIα has been specifically impaired.
Since CaMKIIα is considered to be involved in higher brain functions including learning and memory, and inhibit epileptic seizure and brain disorders caused by ischemia, the inactive CaMKIIα knockin animal of the present invention is widely usable for various studies of the brain and nerve, such as the studies of mechanisms of learning disability, dysmnesia, epileptic seizure and brain disorders.
|Scope of claims||
1. An inactive Ca2+/calmodulin-dependent protein kinase IIalpha (CaMKIIalpha ) knockin nonhuman animal, wherein a CaMKIIalpha gene of one or both of homologous chromosomes is substituted into an inactive type so that an inactive CaMKIIalpha is expressed, wherein lysine corresponding to Lys-42 in the catalytic domain of mouse CaMKIIalpha is substituted by arginine; and thereby a protein kinase activity of CaMKIIalpha is specifically impaired while a calmodulin binding capacity of CaMKIIalpha and a capacity of multimerizing subunits are maintained, wherein the nucleus accumbens of the brain of the inactive CaMKIIalpha knockin nonhuman animal has lower neuronal activity as compared to that of a wild-type, while there is no substantial difference in neuronal activities in the cerebral cortex and caudate-putamen as compared to those of the wild-type,
and wherein the inactive CaMKIIalpha knockin nonhuman animal is produced by a gene targeting method.
2. The inactive CaMKIIalpha knockin nonhuman animal of claim 1, wherein the inactive CaMKIIalpha knockin nonhuman animal is a rodent animal.
3. The inactive CaMKIIalpha knockin nonhuman animal of claim 2, wherein the inactive CaMKIIalpha knockin nonhuman animal is a mouse.
|IPC(International Patent Classification)||
|Reference ( R and D project )||CREST Understanding the Brain AREA|
Contact Information for " Inactive Ca2+/calmodulin-dependent protein kinase IIα knockin animal and knockin cell of the same "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
- Fax: 81-3-5214-8476