Tobacco BY2 cells containing a tomato mosaic virus vector for protein production
| 外国特許コード | F110003325 |
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| 整理番号 | A181-07WO |
| 掲載日 | 2011年6月22日 |
| 出願国 | アメリカ合衆国 |
| 出願番号 | 57411204 |
| 公報番号 | 20090017490 |
| 公報番号 | 8507220 |
| 出願日 | 平成16年10月1日(2004.10.1) |
| 公報発行日 | 平成21年1月15日(2009.1.15) |
| 公報発行日 | 平成25年8月13日(2013.8.13) |
| 国際出願番号 | JP2004014487 |
| 国際公開番号 | WO2005033306 |
| 国際出願日 | 平成16年10月1日(2004.10.1) |
| 国際公開日 | 平成17年4月14日(2005.4.14) |
| 優先権データ |
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| 発明の名称 （英語） | Tobacco BY2 cells containing a tomato mosaic virus vector for protein production |
| 発明の概要（英語） |
(US8507220) An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP. |
| 特許請求の範囲（英語） |
[claim1] 1. A process for producing a tobacco BY2 cell for protein production, comprising: a first transforming step of transfecting tobacco BY2 cells with a plasmid, the plasmid comprising a transcription factor-expressing DNA comprising a coding gene of a transcription factor, wherein the transcription factor is LexA-VP16-hER which is activated by estrogen; and a promoter for expressing the transcription factor; a screening step of screening tobacco BY2 cells, obtained in the first transforming step, for an individual tobacco BY2 cell expressing the transcription factor; and a second transforming step of transfecting the individual tobacco BY2 cell, obtained in the screening step, with a plasmid, the plasmid comprising a protein-expressing DNA comprising cDNA of a RNA virus vector having incorporated therein a gene encoding a target protein, wherein the RNA virus vector is a tomato mosaic virus vector; an inducible promoter which is OLexA-46 located upstream of the cDNA of the RNA virus vector, wherein the virus vector cDNA is transcribed under the control of the inducible promoter; and a ribozyme sequence of satellite tobacco ringspot virus directly ligated to the 3' end of the RNA virus vector cDNA with no sequence ligated therebetween. [claim2] 2. A process for producing a tobacco BY2 cell for protein production as set forth in claim 1, wherein the gene encoding the target protein replaces a viral coat protein gene. [claim3] 3. A process for producing a tobacco BY2 cell for protein production as set forth in claim 1, wherein the transcription factor-expressing DNA and the protein-expressing DNA are transfected by an Agrobacterium method. [claim4] 4. A plant culture tobacco BY2 cell for protein production, which is produced by the process for producing a tobacco BY2 cell for protein production as set forth in claim 1. [claim5] 5. A protein producing process comprising, culturing the tobacco BY2 cell of claim 4 in the presence of estrogen. |
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| 国際特許分類(IPC) |
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| 参考情報 （研究プロジェクト等） | CREST Plants Function and Their Control AREA |
日本語項目の表示
| 発明の名称 | 形質転換細胞および該細胞を用いたタンパク質の生産方法並びにタンパク質生産キット、ウイルスベクター発現用ＤＮＡ断片およびその利用、タンパク質生産用形質転換体の生産方法および当該生産方法によって得られたタンパク質生産用形質転換体並びにその利用 |
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