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Detection instrument with the use of polynucleotides mapped on barley chromosome 実績あり

外国特許コード F110003326
整理番号 A181-11WO
掲載日 2011年6月22日
出願国 アメリカ合衆国
出願番号 54728405
公報番号 20090186402
公報番号 7977087
出願日 平成17年3月31日(2005.3.31)
公報発行日 平成21年7月23日(2009.7.23)
公報発行日 平成23年7月12日(2011.7.12)
国際出願番号 JP2005006825
国際公開番号 WO2005095603
国際出願日 平成17年3月31日(2005.3.31)
国際公開日 平成17年10月13日(2005.10.13)
優先権データ
  • 特願2004-101618 (2004.3.31) JP
  • 特願2004-101626 (2004.3.31) JP
  • 特願2004-101641 (2004.3.31) JP
  • 特願2004-101646 (2004.3.31) JP
  • 特願2004-101662 (2004.3.31) JP
  • 特願2004-101675 (2004.3.31) JP
  • 特願2004-101682 (2004.3.31) JP
  • 特願2004-341944 (2004.11.26) JP
  • 特願2004-342018 (2004.11.26) JP
  • 特願2004-342097 (2004.11.26) JP
  • 特願2004-342261 (2004.11.26) JP
  • 特願2004-342406 (2004.11.26) JP
  • 特願2004-342458 (2004.11.26) JP
  • 特願2004-342598 (2004.11.26) JP
  • 2005WO-JP06825 (2005.3.31) WO
発明の名称 (英語) Detection instrument with the use of polynucleotides mapped on barley chromosome 実績あり
発明の概要(英語) (US7977087)
As the results of mass sequencing of cDNA clones originating in barley varieties “H. spontaneum”, “Haruna Nijo” and “Akashinriki”, a large number of sites showing single nucleotide polymorphisms (SNPs) among the varieties are found out.
Because of involving nonsynonymous substitutions and likely relating to phenotypes inherent in respective varieties, these SNPs are usable for various purposes, for example, genetically distinguishing a variety, isolating a gene, producing/selecting a novel transformant and so on.
特許請求の範囲(英語) [claim1]
1. A gene detection instrument for detecting expression or polymorphism of genes existing in a genome of Triticeae species, said gene detection instrument comprising a support on which are immobilized at least 50 polynucleotides selected from the group consisting of:
(a) polynucleotides with partial base sequences of chromosomal DNA of barley and
(b) polynucleotides in which a base sequence constituting part of entire base sequences of chromosomal DNA of barley is joined to non-continuous base sequences constituting other parts of the entire base sequences of chromosomal DNA of barley wherein the polynucleotide immobilized on the support comprises; at least one kind of polynucleotide is selected from the group consisting of:
(1) polynucleotides with the base sequences of SEQ ID NO: 1 through 5780;
(2) a polynucleotide whose partial sequence comprises all of any one of the polynucleotides set forth in (1); and
(3) a polynucleotide whose partial sequence comprises: all of a base sequence of SEQ ID NO: n (where n is an odd number), or in the polynucleotides set forth in (1), and all of a base sequence of SEQ ID NO: n+1, or in the polynucleotides set forth in (1); the polynucleotides being mapped on barley chromosomes for an entire genome of barley, and
the polynucleotides being mapped on the support at an average density of no greater than 30 cM;
regions on the support in which said at least 50 polynucleotides are respectively immobilized being arranged in the same order as a chromosomal order of said at least 50 polynucleotides immobilized on the support, or
information indicative of a chromosomal order of said at least 50 polynucleotides immobilized on the support being appended to regions on the support in which said at least 50 polynucleotides are respectively immobilized.
[claim2]
2. A gene detection instrument as set forth in claim 1, wherein the polynucleotide immobilized on the support comprises cDNA.
  • 発明者/出願人(英語)
  • TAKEDA KAZUYOSHI
  • SATO KAZUHIRO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Plants Function and Their Control AREA
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