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Plant having increased yield of seeds 実績あり

外国特許コード F110003330
整理番号 A181-20WO
掲載日 2011年6月22日
出願国 アメリカ合衆国
出願番号 52328308
公報番号 20100083404
公報番号 8173865
出願日 平成20年1月15日(2008.1.15)
公報発行日 平成22年4月1日(2010.4.1)
公報発行日 平成24年5月8日(2012.5.8)
国際出願番号 JP2008050341
国際公開番号 WO2008087932
国際出願日 平成20年1月15日(2008.1.15)
国際公開日 平成20年7月24日(2008.7.24)
優先権データ
  • 特願2007-007464 (2007.1.16) JP
  • 2008WO-JP50341 (2008.1.15) WO
発明の名称 (英語) Plant having increased yield of seeds 実績あり
発明の概要(英語) (US8173865)
It is found that a transformed plant into which a γ-glutamylcysteine synthetase gene has been introduced is increased in at least one of the number of flowers and the number of seeds in comparison with a wild-type plant, and is therefore increased in seed yield.
Based on this finding, the present invention elucidates the function of a product of a plant-derived γ-glutamylcysteine synthetase gene and provides a technique for producing a plant having an increased seed yield.
特許請求の範囲(英語) [claim1]
1. A plant transformed by introducing a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase, the polynucleotide producing a translated product having no chloroplast targeting signal peptide,
the plant being further increased in biomass quantity per unit area or seed yield per unit area in comparison with a parent plant thereof by being cultivated at a planting density higher than that which allows increases in the biomass quantity per unit area and in the seed yield per unit area
wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d):
(a) a polynucleotide encoding a polypeptide having the amino-acid sequence comprising SEQ ID NO: 3;
(b) a polynucleotide encoding a polypeptide having an amino-acid sequence in which not more than 10 amino acids of which are deleted, substituted, or added from/in/to the amino-acid sequence comprising SEQ ID NO: 3;
(c) a polynucleotide having the base sequence comprising SEQ ID NO: 4; and
(d) a polynucleotide that hybridizes, under stringent conditions, with the polynucleotide having the base sequence comprising SEQ ID NO: 4.
[claim2]
2. The transformed plant according to claim 1, into which a polynucleotide encoding glutathione-binding aldolase is further introduced.
[claim3]
3. A method for increasing the number of flowers of a plant and/or the number of seeds of the plant, the method comprising the steps of: introducing, into the plant, a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase, wherein the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (h):
(a) a polynucleotide encoding a polypeptide having the amino-acid sequence comprising SEQ ID NO: 1;
(b) a polynucleotide encoding a polypeptide having an amino-acid sequence in which not more than 10 amino acids of which are deleted, substituted, or added from/in/to the amino-acid sequence comprising SEQ ID NO: 1;
(c) a polynucleotide having the base sequence comprising SEQ ID NO: 2;
(d) a polynucleotide that hybridizes, under stringent conditions, with the polynucleotide having the base sequence comprising SEQ ID NO: 2;
(e) a polynucleotide encoding a polypeptide having the amino-acid sequence comprising SEQ ID NO: 3;
(f) a polynucleotide encoding a polypeptide having an amino-acid sequence in which not more than 10 amino acids of which are deleted, substituted, or added from/in/to the amino-acid sequence comprising SEQ ID NO: 3;
(g) a polynucleotide having the base sequence comprising SEQ ID NO: 4; and
(h) a polynucleotide that hybridizes, under stringent conditions, with the polynucleotide having the base sequence comprising SEQ ID NO: 4; and
cultivating the plant, into which the polynucleotide is introduced, at a planting density higher than that which allows increases in biomass quantity per unit area and in seed yield per unit area.
[claim4]
4. A method for increasing biomass quantity or seed yield of a plant per unit area, the method comprising the steps of: introducing, into the plant, a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase; and
cultivating the plant, into which the polynucleotide is introduced, at a planting density higher than that which allows increases in biomass quantity per unit area and in seed yield per unit area,
wherein a translated product of the polynucleotide encoding the plant-derived gamma -glutamylcysteine synthetase has a chloroplast targeting signal peptide or
wherein a translated product of the polynucleotide encoding the plant-derived gamma -glutamylcysteine synthetase does not have a chloroplast targeting signal peptide and a harvest index is improved.
[claim5]
5. The method according to claim 4, wherein the translated product of the polynucleotide encoding the plant-derived gamma -glutamylcysteine synthetase has a chloroplast targeting signal peptide, and the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d): (a) a polynucleotide encoding a polypeptide having the amino-acid sequence comprising SEQ ID NO: 1;
(b) a polynucleotide encoding a polypeptide having an amino-acid sequence in which not more than 10 amino acids of which are deleted, substituted, or added from/in/to the amino-acid sequence comprising SEQ ID NO: 1;
(c) a polynucleotide having the base sequence comprising SEQ ID NO: 2; and
(d) a polynucleotide that hybridizes, under stringent conditions, with the polynucleotide having the base sequence comprising SEQ ID NO: 2.
[claim6]
6. The method according to claim 4, wherein the translated product of the polynucleotide encoding the plant-derived gamma -glutamylcysteine synthetase does not have a chloroplast targeting signal peptide and a harvest index is improved, and the polynucleotide encoding the gamma -glutamylcysteine synthetase is selected from the group consisting of the following (a) to (d): (a) a polynucleotide encoding a polypeptide having the amino-acid sequence comprising SEQ ID NO: 3;
(b) a polynucleotide encoding a polypeptide having an amino-acid sequence in which not more than 10 amino acids of which are deleted, substituted, or added from/in/to the amino-acid sequence comprising SEQ ID NO: 3;
(c) a polynucleotide having the base sequence comprising SEQ ID NO: 4; and
(d) a polynucleotide that hybridizes, under stringent conditions, with the polynucleotide having the base sequence comprising SEQ ID NO: 4.
[claim7]
7. The method according to claim 4, further comprising the step of introducing into the plant a polynucleotide encoding glutathione-binding aldolase.
[claim8]
8. A method for producing a plant that is increased in biomass quantity per unit area or seed yield per unit area by being cultivated at a planting density higher than that which allows increases in the biomass quantity per unit area and in the seed yield per unit area, the method comprising the steps of: introducing into the plant a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase; and
selecting a plant having an increased biomass or number of flowers and/or seeds in comparison with its parent plant under a cultivation condition where the planting density was higher than that which allows increases in biomass quantity per unit area and in seed yield per unit area.
[claim9]
9. The method according to claim 8, further comprising the step of introducing into the plant a polynucleotide encoding glutathione-binding aldolase.
[claim10]
10. A plant transformed by introducing a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase and further introducing a polynucleotide encoding glutathione-binding aldolase, the polynucleotide producing a translated product having no chloroplast targeting signal peptide,
the plant being further increased in biomass quantity per unit area or seed yield per unit area in comparison with a parent plant thereof by being cultivated at a planting density higher than that which allows increases in the biomass quantity per unit area and in the seed yield per unit area.
[claim11]
11. A method for increasing biomass quantity or seed yield of a plant per unit area, the method comprising the steps of: introducing, into the plant, a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase;
introducing into the plant a polynucleotide encoding glutathione-binding aldolase; and
cultivating the plant, into which the polynucleotide is introduced, at a planting density higher than that which allows increases in biomass quantity per unit area and in seed yield per unit area.
[claim12]
12. A method for producing a plant that is increased in biomass quantity per unit area or seed yield per unit area by being cultivated at a planting density higher than that which allows increases in the biomass quantity per unit area and in the seed yield per unit area, the method comprising the steps of: introducing into the plant a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase; and
introducing into the plant a polynucleotide encoding glutathione-binding aldolase.
[claim13]
13. A plant transformed by introducing a polynucleotide encoding a plant-derived gamma -glutamylcysteine synthetase, the polynucleotide producing a translated product having no chloroplast targeting signal peptide, the plant being cultivated at a planting density higher than that which allows increases in the biomass quantity per unit area and in the seed yield per unit area,
wherein the plant having an increased biomass or number of flowers and/or seeds in comparison with its parent plant under a cultivation condition where the planting density was higher than that which allows increases in biomass quantity per unit area and in seed yield per unit area is selected.
  • 発明者/出願人(英語)
  • OGAWA KENICHI
  • IWASAKI AYA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • OKAWAMA PREFECTURE
  • OKAYAMA PREFECTURE
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Plants Function and Their Control AREA
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