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Virus vector and use thereof

外国特許コード F110003332
整理番号 A181-23WO
掲載日 2011年6月22日
出願国 アメリカ合衆国
出願番号 45099908
公報番号 20110107466
公報番号 8586826
出願日 平成20年4月11日(2008.4.11)
公報発行日 平成23年5月5日(2011.5.5)
公報発行日 平成25年11月19日(2013.11.19)
国際出願番号 JP2008057170
国際公開番号 WO2008136253
国際出願日 平成20年4月11日(2008.4.11)
国際公開日 平成20年11月13日(2008.11.13)
優先権データ
  • 特願2007-120181 (2007.4.27) JP
  • 2008WO-JP57170 (2008.4.11) WO
発明の名称 (英語) Virus vector and use thereof
発明の概要(英語) (US8586826)
It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition.
By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.
特許請求の範囲(英語) [claim1]
1. A polynucleotide comprising a tobamoviral nucleotide sequence, the tobamoviral nucleotide sequence comprising: a first nucleotide sequence encoding a tobamoviral replication protein;
a second nucleotide sequence encoding a tobamoviral movement protein, the second nucleotide sequence being located downstream of the first nucleotide sequence; and
a linking site for linking with an exogenous nucleotide sequence encoding a polypeptide to be expressed, wherein the linking site is located downstream of the second nucleotide sequence,
and wherein the second nucleotide sequence comprises a mutation relative to the wild-type nucleotide sequence encoding the tobamoviral movement protein, wherein the mutation comprises an insertion of 300 or more contiguous nucleotides, and wherein the insertion is a sequence other than an intron.
[claim2]
7. A plant comprising the polynucleotide recited in claim 1.
[claim3]
2. The polynucleotide according to claim 1, wherein the second nucleotide sequence comprises the mutation at any position from 17 to 795 of the nucleotide sequence shown in SEQ ID NO: 20.
[claim4]
3. The polynucleotide according to claim 1, wherein the first nucleotide sequence encoding a tobamoviral replication protein is:
(i) a polynucleotide encoding a polypeptide having at least 80% identity to SEQ ID NO: 1; or
(ii) a polynucleotide encoding a polypeptide having at least 80% identity to SEQ ID NO: 2.
[claim5]
4. The polynucleotide according to claim 1, wherein the second nucleotide sequence is selected from the group consisting of polynucleotides having at least 80% identity to SEQ ID NO: 13 and a polynucleotide which hybridizes with a polynucleotide having a sequence that is complementary to SEQ ID NO: 13 under a stringent condition for hybridization.
[claim6]
5. The polynucleotide according to claim 1, wherein the tobamoviral replication protein and the tobamoviral movement protein are derived from a tobacco mosaic virus or a tomato mosaic virus.
[claim7]
6. A vector comprising the polynucleotide recited in claim 1.
[claim8]
8. A plant comprising the vector recited in claim 6.
[claim9]
9. A transformant comprising the polynucleotide recited in claim 1.
[claim10]
10. A transformant comprising the vector recited in claim 6.
[claim11]
11. A method for producing a polypeptide, comprising: transforming or transfecting a plant with the polynucleotide recited in claim 1.
[claim12]
12. A method for producing a polypeptide, comprising the step of: transforming a cell with the polynucleotide recited in claim 1.
[claim13]
13. A kit for producing a polypeptide, comprising the polynucleotide recited in claim 1.
[claim14]
14. A method for producing a polypeptide, comprising the step of: transforming or transfecting a plant with the vector recited in claim 6.
[claim15]
15. A method for producing a polypeptide, comprising the step of: transforming a cell with the vector recited in claim 6.
[claim16]
16. A kit for producing a polypeptide, comprising the vector recited in claim 6.
[claim17]
17. A method for producing a polypeptide, comprising the step of: using the plant recited in claim 7.
[claim18]
18. A method for producing a polypeptide, comprising the step of: using the transformant recited in claim 9.
[claim19]
19. A kit for producing a polypeptide, comprising the plant recited in claim 7.
[claim20]
20. A kit for producing a polypeptide, comprising the transformant recited in claim 9.
[claim21]
21. A polynucleotide comprising a tobamoviral nucleotide sequence, the tobamoviral nucleotide sequence comprising: a first nucleotide sequence encoding a tobamoviral replication protein;
a second nucleotide sequence encoding a tobamoviral movement protein, the second nucleotide sequence being located downstream of the first nucleotide sequence; and
a linking site for linking with an exogenous nucleotide sequence encoding a polypeptide to be expressed, wherein the linking site is located downstream of the second nucleotide sequence, and wherein the second nucleotide sequence comprises a mutation relative to the wild-type nucleotide sequence encoding the tobamoviral movement protein, wherein the mutation comprises an insertion of 1258 or more contiguous nucleotides.
  • 発明者/出願人(英語)
  • MORI MASASHI
  • DOHI KOJI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Plants Function and Their Control AREA
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