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Virus vector and use thereof

Foreign code F110003332
File No. A181-23WO
Posted date Jun 22, 2011
Country United States of America
Application number 45099908
Gazette No. 20110107466
Gazette No. 8586826
Date of filing Apr 11, 2008
Gazette Date May 5, 2011
Gazette Date Nov 19, 2013
International application number JP2008057170
International publication number WO2008136253
Date of international filing Apr 11, 2008
Date of international publication Nov 13, 2008
Priority data
  • P2007-120181 (Apr 27, 2007) JP
  • 2008WO-JP57170 (Apr 11, 2008) WO
Title Virus vector and use thereof
Abstract (US8586826)
It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition.
By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.
Scope of claims [claim1]
1. A polynucleotide comprising a tobamoviral nucleotide sequence, the tobamoviral nucleotide sequence comprising: a first nucleotide sequence encoding a tobamoviral replication protein;
a second nucleotide sequence encoding a tobamoviral movement protein, the second nucleotide sequence being located downstream of the first nucleotide sequence; and
a linking site for linking with an exogenous nucleotide sequence encoding a polypeptide to be expressed, wherein the linking site is located downstream of the second nucleotide sequence,
and wherein the second nucleotide sequence comprises a mutation relative to the wild-type nucleotide sequence encoding the tobamoviral movement protein, wherein the mutation comprises an insertion of 300 or more contiguous nucleotides, and wherein the insertion is a sequence other than an intron.
[claim2]
7. A plant comprising the polynucleotide recited in claim 1.
[claim3]
2. The polynucleotide according to claim 1, wherein the second nucleotide sequence comprises the mutation at any position from 17 to 795 of the nucleotide sequence shown in SEQ ID NO: 20.
[claim4]
3. The polynucleotide according to claim 1, wherein the first nucleotide sequence encoding a tobamoviral replication protein is:
(i) a polynucleotide encoding a polypeptide having at least 80% identity to SEQ ID NO: 1; or
(ii) a polynucleotide encoding a polypeptide having at least 80% identity to SEQ ID NO: 2.
[claim5]
4. The polynucleotide according to claim 1, wherein the second nucleotide sequence is selected from the group consisting of polynucleotides having at least 80% identity to SEQ ID NO: 13 and a polynucleotide which hybridizes with a polynucleotide having a sequence that is complementary to SEQ ID NO: 13 under a stringent condition for hybridization.
[claim6]
5. The polynucleotide according to claim 1, wherein the tobamoviral replication protein and the tobamoviral movement protein are derived from a tobacco mosaic virus or a tomato mosaic virus.
[claim7]
6. A vector comprising the polynucleotide recited in claim 1.
[claim8]
8. A plant comprising the vector recited in claim 6.
[claim9]
9. A transformant comprising the polynucleotide recited in claim 1.
[claim10]
10. A transformant comprising the vector recited in claim 6.
[claim11]
11. A method for producing a polypeptide, comprising: transforming or transfecting a plant with the polynucleotide recited in claim 1.
[claim12]
12. A method for producing a polypeptide, comprising the step of: transforming a cell with the polynucleotide recited in claim 1.
[claim13]
13. A kit for producing a polypeptide, comprising the polynucleotide recited in claim 1.
[claim14]
14. A method for producing a polypeptide, comprising the step of: transforming or transfecting a plant with the vector recited in claim 6.
[claim15]
15. A method for producing a polypeptide, comprising the step of: transforming a cell with the vector recited in claim 6.
[claim16]
16. A kit for producing a polypeptide, comprising the vector recited in claim 6.
[claim17]
17. A method for producing a polypeptide, comprising the step of: using the plant recited in claim 7.
[claim18]
18. A method for producing a polypeptide, comprising the step of: using the transformant recited in claim 9.
[claim19]
19. A kit for producing a polypeptide, comprising the plant recited in claim 7.
[claim20]
20. A kit for producing a polypeptide, comprising the transformant recited in claim 9.
[claim21]
21. A polynucleotide comprising a tobamoviral nucleotide sequence, the tobamoviral nucleotide sequence comprising: a first nucleotide sequence encoding a tobamoviral replication protein;
a second nucleotide sequence encoding a tobamoviral movement protein, the second nucleotide sequence being located downstream of the first nucleotide sequence; and
a linking site for linking with an exogenous nucleotide sequence encoding a polypeptide to be expressed, wherein the linking site is located downstream of the second nucleotide sequence, and wherein the second nucleotide sequence comprises a mutation relative to the wild-type nucleotide sequence encoding the tobamoviral movement protein, wherein the mutation comprises an insertion of 1258 or more contiguous nucleotides.
  • Inventor, and Inventor/Applicant
  • MORI MASASHI
  • DOHI KOJI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) CREST Plants Function and Their Control AREA
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