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Method for the synthesis of nucleic acid without protecting base moiety

Foreign code F110003371
File No. A251-11WO
Posted date Jun 23, 2011
Country United States of America
Application number 59117205
Gazette No. 07807821
Date of filing Apr 26, 2007
Gazette Date Oct 5, 2010
International application number PCT/JP2005/003053
International publication number WO2005/082923
Date of international filing Feb 24, 2005
Date of international publication Sep 9, 2005
Priority data
  • P2004-056707 (Mar 1, 2004) JP
Title Method for the synthesis of nucleic acid without protecting base moiety
Abstract A phosphoramidite method for the synthesis of a nucleic acid oligomer without protecting the base moiety characterized in that a 3' or 5' hydroxyl group of a nucleotide is reacted with a nucleoside phosphoramidite, a cyclonucleoside phosphoramidite, a 2'-substituted nucleoside phosphoramidite, a 4'-substituted nucleoside phosphoramidite, or a 2',4'-di-substituted nucleoside phosphoramidite to produce a phosphodiester linkage. The phosphoramidite is contacted with an activator containing both a) hydroxybenzotriazole-1-ol (HOBt), a mono-substituted HOBt, a di-substituted HOBt, or a di-substituted phenol and b) imidazole, tetrazole, benzimidazoletriflate (BIT), 4-ethylthiotetrazole, imidazolium triflate(trifluoromethane sulfonate) or 4,5-dicyanoimidazole.
  • Inventor, and Inventor/Applicant
  • Sekine, Mitsuo; Yokohama [JP]
  • Seio, Kohji; Yokohama [JP]
  • Ohkubo, Akihiro; Machida [JP]
  • Japan Science and Technology Agency, Kawaguchi-shi [JP]
IPC(International Patent Classification)
Reference ( R and D project ) CREST Creation of Bio-Devices and Bio-Systems with Chemical and Biological Molecules for Medical Use AREA
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