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Immunostimulating agents 実績あり

外国特許コード F110003530
整理番号 B18-01WO
掲載日 2011年6月28日
出願国 アメリカ合衆国
出願番号 55710804
公報番号 20080262210
公報番号 7790189
出願日 平成16年5月13日(2004.5.13)
公報発行日 平成20年10月23日(2008.10.23)
公報発行日 平成22年9月7日(2010.9.7)
国際出願番号 JP2004006793
国際公開番号 WO2004100965
国際出願日 平成16年5月13日(2004.5.13)
国際公開日 平成16年11月25日(2004.11.25)
優先権データ
  • 特願2003-136876 (2003.5.15) JP
  • 2004WO-JP06793 (2004.5.13) WO
発明の名称 (英語) Immunostimulating agents 実績あり
発明の概要(英語) (US7790189)
Disclosed is a new type of immunostimulating agent including an immunostimulating oligonucleotide complexed with a carrier which is safe and has a high transfection effect.
The carrier complexed with the immunostimulating oligonucleotide to form the immunostimulating agent is a polysaccharide having &bgr;-1,3-bonds (preferably &bgr;-1,3-glucan such as schizophyllan).
A preferred example of the immunostimulating oligonucleotide is one containing an unmethylated CpG motif.
The polysaccharide for use is preferably modified with nucleic acid-binding functional group and/or cell membrane-affinitive functional group.
特許請求の範囲(英語) [claim1]
1. An immunostimulating agent which comprises a complex of an immunostimulating oligonucleotide of 8 to 100 nucleotides which contains an unmethylated CpG motif and a polysaccharide having beta -1,3-bonds.
[claim2]
2. The immunostimulating agent of claim 1, wherein the phosphoric acid backbone of the oligonucleotide is phosphorothioate-modified or phosphorodithioate-modified.
[claim3]
3. The immunostimulating agent of claim 1, wherein the polysaccharide having beta -1,3-bonds is beta -1,3-glucan or beta -1,3-xylan.
[claim4]
4. The immunostimulating agent of claim 1, wherein the beta -1,3-glucan is selected from among schizophyllan, curdlan, lentinan, pachyman, grifolan, laminaran and scleroglucan.
[claim5]
5. The immunostimulating agent of claim 1, wherein the polysaccharide is modified with nucleic acid-binding functional group and/or cell membrane-affinitive functional group.
[claim6]
6. The immunostimulating agent of claim 1, wherein the complex of the oligonucleotide and the polysaccharide is of a triple helix structure formed through hydrogen bonds and hydrophobic interactions.
[claim7]
7. The immunostimulating agent of claim 1, wherein said unmethylated CpG motif is selected the group consisting of AACGTT, AGCGTT, GACGTT, GGCGTT, AACGTC, AGCGTC, GACGTC, GGCGTC, AACGCC, AGCGCC, GACGCC, GGCGCC, AACGCT, AGCGCT, GACGCT, and GGCGCT.
[claim8]
8. The immunostimulating agent of claim 1, wherein said immunostimulating oligonucleotide is selected from the group consisting of:
(SEQ ID NO 7)accgataccggtgccggtgacggcaccacg;(SEQ ID NO 8)accgatagcgctgccggtgacggcaccacg;(SEQ ID NO 9)accgatgacgtcgccggtgacggcaccacg;(SEQ ID NO 10)accgattcgcgagccggtgacggcaccacg;(SEQ ID NO 11)ggggggggggggcgatcggggggggggggg;(SEQ ID NO 12)gggggggggggacgatcgtcgggggggggg;(SEQ ID NO 13)ggggggggggggaacgttgggggggggggg;(SEQ ID NO 14)GAGAACGCTCGACCTTCGAT;(SEQ ID NO 15)TCCATGACGTTCCTGATGCT; and(SEQ ID NO 16)TCTCCCAGCGTGCGCCAT;
wherein capital letters denote a thiolated DNA.
[claim9]
8. The immunostimulating agent of
-- (SEQ ID NO 7)
-- accgataccggtgccggtgacggcaccacg;
--
-- (SEQ ID NO 8)
-- accgatagcgctgccggtgacggcaccacg;
--
-- (SEQ ID NO 9)
-- accgatgacgtcgccggtgacggcaccacg;
--
-- (SEQ ID NO 10)
-- accgattcgcgagccggtgacggcaccacg;
--
-- (SEQ ID NO 11)
-- ggggggggggggcgatcggggggggggggg;
--
-- (SEQ ID NO 12)
-- gggggggggggacgatcgtcgggggggggg;
--
-- (SEQ ID NO 13)
-- ggggggggggggaacgttgggggggggggg;
--
-- (SEQ ID NO 14)
-- GAGAACGCTCGACCTTCGAT;
--
-- (SEQ ID NO 15)
-- TCCATGACGTTCCTGATGCT; and
--
-- (SEQ ID NO 16)
-- TCTCCCAGCGTGCGCCAT;
[claim10]
9. The immunostimulating agent of claim 1, wherein the polysaccharide to be complexed is provided with nucleic acid-binding functional groups formed by periodate oxidation of 1,6-glucopyranoside branches followed by reductive amination.
  • 発明者/出願人(英語)
  • MIZU MASAMI
  • SHINKAI SEIJI
  • SAKURAI KAZUO
  • KOUMOTO KAZUYA
  • NUMATA MUNENORI
  • MATSUMOTO TAKAHIRO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • MITSUI SUGAR
国際特許分類(IPC)
参考情報 (研究プロジェクト等) SORST Selected in Fiscal 2001
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