Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein
|Posted date||Jun 30, 2011|
|Country||United States of America|
|Date of filing||May 31, 2007|
|Gazette Date||Oct 23, 2008|
|Gazette Date||Oct 26, 2010|
|International application number||JP1998000430|
|International publication number||WO1998033900|
|Date of international filing||Feb 2, 1998|
|Date of international publication||Aug 6, 1998|
|Title||Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein|
The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known.
More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator.
The present also provides a system for predicting a functional site for automatically performing said methods.
Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.
|Scope of claims||
1. A thermophilic DNA polymerase consisting of the amino acid sequence of SEQ ID NO: 6.
|IPC(International Patent Classification)|
|Reference ( R and D project )||ERATO DOI Bioasymmetry AREA|
Contact Information for " Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
- Fax: 81-3-5214-8476