Viral vector and use thereof

外国特許コード	F110003610
整理番号	A181-23WO
掲載日	2011年6月30日
出願国	欧州特許庁（ＥＰＯ）
出願番号	08740267
公報番号	2154246
公報番号	2154246
出願日	平成20年4月11日(2008.4.11)
公報発行日	平成22年2月17日(2010.2.17)
公報発行日	平成29年3月29日(2017.3.29)
国際出願番号	JP2008057170
国際公開番号	WO2008136253
国際出願日	平成20年4月11日(2008.4.11)
国際公開日	平成20年11月13日(2008.11.13)
優先権データ	特願2007-120181 (2007.4.27) JP 2008WO-JP57170 (2008.4.11) WO
発明の名称 （英語）	Viral vector and use thereof
発明の概要（英語）	(EP2154246) It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.
特許請求の範囲（英語）	[claim1] 1. A polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein; and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, and the linking site being located downstream of the second base sequence, the second base sequence comprising an inserted base sequence having a base length of 100 or more at any position from 17th base to 795th base of the base sequence shown in SEQ ID NO: 20, wherein a polynucleotide having the second base sequence is: (i) a polynucleotide having the base sequence shown in any one of SEQ ID NO: 4 through 17. [claim2] 2. The polynucleotide according to any one of claims 1, wherein the viral replication protein is: (i) polypeptides having amino acid sequences shown in SEQ ID NO: 1 and 2, respectively. [claim3] 3. The polynucleotide according to any of claims 1 or 2, wherein the virus belongs to a tobamovirus. [claim4] 4. The polynucleotide according to any of claims 1 to 3, wherein the virus is a tobacco mosaic virus or a tomato mosaic virus. [claim5] 5. A vector comprising a polynucleotide recited in any of claims 1 to 4. [claim6] 6. A plant comprising a polynucleotide recited in any of claims 1 to 4. [claim7] 7. A plant comprising a vector recited in claim 5. [claim8] 8. A transformant comprising a polynucleotide recited in claims 1 to 4. [claim9] 9. A transformant comprising a vector recited in claim 5. [claim10] 10. A method for producing a polypeptide, comprising: transforming or transfecting a plant with a polynucleotide recited in any of claims 1 to 4. [claim11] 11. A method for producing a polypeptide, comprising the step of: transforming a cell with a polynucleotide recited in any of claims 1 to 4. [claim12] 12. A kit for producing a polypeptide, comprising a polynucleotide recited in any of claims 1 to 4. [claim13] 13. A method for producing a polypeptide, comprising the step of: transforming or transfecting a plant with a vector recited in claim 5. [claim14] 14. A method for producing a polypeptide, comprising the step of: transforming a cell with a vector recited in claim 5. [claim15] 15. A kit for producing a polypeptide, comprising a vector recited in claim 5. [claim16] 16. A method for producing a polypeptide, comprising the step of: using a plant recited in claim 6 or claim 7. [claim17] 17. A method for producing a polypeptide, comprising the step of: using a transformant recited in claim 8 or claim 9. [claim18] 18. A kit for producing a polypeptide, comprising a plant recited in claim 6 or 7. [claim19] 19. A kit for producing a polypeptide, comprising a transformant recited in claim 8 or 9.
出願人（英語）	JAPAN SCIENCE AND TECHNOLOGY AGENCY
発明者（英語）	MORI MASASHI DOHI KOJI
国際特許分類(IPC)	A01H   5/00      開花植物，すなわち，被子植物 C12N   1/15      外来遺伝物質の導入によって修飾されたもの C12N   1/19      外来遺伝物質の導入によって修飾されたもの C12N   1/21      外来遺伝物質の導入によって修飾されたもの C12N   5/10      外来遺伝物質の導入によって修飾された細胞，例．ウイルス形質転換細胞 C12N  15/09      組換えＤＮＡ技術 C12P  21/02      ２以上のアミノ酸の結合順序が既知のもの，例．グルタチオン
参考情報 （研究プロジェクト等）	CREST Plants Function and Their Control AREA