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Viral vector and use thereof

Foreign code F110003610
File No. A181-23WO
Posted date Jun 30, 2011
Country EPO
Application number 08740267
Gazette No. 2154246
Gazette No. 2154246
Date of filing Apr 11, 2008
Gazette Date Feb 17, 2010
Gazette Date Mar 29, 2017
International application number JP2008057170
International publication number WO2008136253
Date of international filing Apr 11, 2008
Date of international publication Nov 13, 2008
Priority data
  • P2007-120181 (Apr 27, 2007) JP
  • 2008WO-JP57170 (Apr 11, 2008) WO
Title Viral vector and use thereof
Abstract (EP2154246)
It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition.
By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.
Scope of claims [claim1]
1. A polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein; and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, and the linking site being located downstream of the second base sequence, the second base sequence comprising an inserted base sequence having a base length of 100 or more at any position from 17th base to 795th base of the base sequence shown in SEQ ID NO: 20, wherein a polynucleotide having the second base sequence is: (i) a polynucleotide having the base sequence shown in any one of SEQ ID NO: 4 through 17.
[claim2]
2. The polynucleotide according to any one of claims 1, wherein
the viral replication protein is: (i) polypeptides having amino acid sequences shown in SEQ ID NO: 1 and 2, respectively.
[claim3]
3. The polynucleotide according to any of claims 1 or 2, wherein
the virus belongs to a tobamovirus.
[claim4]
4. The polynucleotide according to any of claims 1 to 3, wherein
the virus is a tobacco mosaic virus or a tomato mosaic virus.
[claim5]
5. A vector comprising a polynucleotide recited in any of claims 1 to 4.
[claim6]
6. A plant comprising a polynucleotide recited in any of claims 1 to 4.
[claim7]
7. A plant comprising a vector recited in claim 5.
[claim8]
8. A transformant comprising a polynucleotide recited in claims 1 to 4.
[claim9]
9. A transformant comprising a vector recited in claim 5.
[claim10]
10. A method for producing a polypeptide, comprising: transforming or transfecting a plant with a polynucleotide recited in any of claims 1 to 4.
[claim11]
11. A method for producing a polypeptide, comprising the step of: transforming a cell with a polynucleotide recited in any of claims 1 to 4.
[claim12]
12. A kit for producing a polypeptide, comprising a polynucleotide recited in any of claims 1 to 4.
[claim13]
13. A method for producing a polypeptide, comprising the step of: transforming or transfecting a plant with a vector recited in claim 5.
[claim14]
14. A method for producing a polypeptide, comprising the step of: transforming a cell with a vector recited in claim 5.
[claim15]
15. A kit for producing a polypeptide, comprising a vector recited in claim 5.
[claim16]
16. A method for producing a polypeptide, comprising the step of: using a plant recited in claim 6 or claim 7.
[claim17]
17. A method for producing a polypeptide, comprising the step of: using a transformant recited in claim 8 or claim 9.
[claim18]
18. A kit for producing a polypeptide, comprising a plant recited in claim 6 or 7.
[claim19]
19. A kit for producing a polypeptide, comprising a transformant recited in claim 8 or 9.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • MORI MASASHI
  • DOHI KOJI
IPC(International Patent Classification)
Reference ( R and D project ) CREST Plants Function and Their Control AREA
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