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Method for determination of presence or absence of peptide compound PYY3-36

Foreign code F110003830
File No. I019P006US1
Posted date Jul 6, 2011
Country United States of America
Application number 46388909
Gazette No. 20100009453
Gazette No. 7993929
Date of filing May 11, 2009
Gazette Date Jan 14, 2010
Gazette Date Aug 9, 2011
Priority data
  • P2006-304529 (Nov 9, 2006) JP
  • 2007WO-JP70785 (Oct 25, 2007) WO
Title Method for determination of presence or absence of peptide compound PYY3-36
Abstract (US7993929)
The present invention provides a method for determining in a pharmaceutical test formulation the presence or absence of a peptide compound PYY3-36 represented by the following amino acid sequence: H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X (SEQ ID NO: 1), wherein X is OH or a carboxy acid-protecting group, the method comprising (1) preparing a solution by mixing the pharmaceutical test formulation with cucurbit[7]uril in a solvent; and (2) thermally analyzing the solution prepared in Step (1).
Scope of claims [claim1]
1. A method for determining in a pharmaceutical test formulation the presence or absence of a peptide compound PYY3-36 represented by the following amino acid sequence: H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X (SEQ ID NO: 1), wherein X is OH or a carboxy acid-protecting group, the method comprising: (1) preparing a solution by mixing the pharmaceutical test formulation with cucurbit[7]uril in a solvent; and
(2) thermally analyzing the solution prepared in Step (1)
wherein generation of heat by the solution prepared in Step (1) evidences the existence of H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X, wherein X is OH or a carboxy acid-protecting group.
[claim2]
2. The determination method according to claim 1, wherein the presence or absence of a peptide compound PYY1-36 represented by the following amino acid sequence: H-Tyr-Pro-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 (SEQ ID NO: 2), and/or peptide digests generated by degradation of the peptide compound PYY1-36 is determined.
[claim3]
3. The determination method according to claim 1, wherein peptide digests generated by degradation of the peptide compound PYY3-36 is detected.
  • Inventor, and Inventor/Applicant
  • INOUE YOSHIHISA
  • REKHARSKY MIKHAIL
  • KIM KIMOON
  • KO YONG HO
  • SELVAPALAM NARAYANAN
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
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