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Protein-responsive translational regulatory system using RNA-protein interacting motif UPDATE

外国特許コード F110003837
整理番号 I029P004WO
掲載日 2011年7月6日
出願国 アメリカ合衆国
出願番号 74385308
公報番号 20110040077
公報番号 9255274
出願日 平成20年11月21日(2008.11.21)
公報発行日 平成23年2月17日(2011.2.17)
公報発行日 平成28年2月9日(2016.2.9)
国際出願番号 JP2008071213
国際公開番号 WO2009066757
国際出願日 平成20年11月21日(2008.11.21)
国際公開日 平成21年5月28日(2009.5.28)
優先権データ
  • 特願2007-303662 (2007.11.22) JP
  • 特願2008-186385 (2008.7.17) JP
  • 2008WO-JP71213 (2008.11.21) WO
発明の名称 (英語) Protein-responsive translational regulatory system using RNA-protein interacting motif UPDATE
発明の概要(英語) (US9255274)
An object of the present invention is to provide a translationally regulatable mRNA which has wider application and can perform specific ON-OFF regulation, an RNA-protein complex specifically bound to the mRNA, and a translational regulatory system.
The present invention provides an mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5′ to the ribosome-binding site or within the 5′ region of the open reading frame, and an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5′ to the ribosome-binding site or within the 5′ region of the open reading frame.
特許請求の範囲(英語) [claim1]
1. An isolated non-naturally occurring mRNA encoding a protein comprising an RNA-protein complex interacting motif nucleotide sequence incorporated 5' to a ribosome-binding site in a position 2 to 10 bases distant from the ribosome-binding site or within the 5' region of an open reading frame, wherein the interacting motif comprises nucleic acid sequence SEQ ID NO:9, wherein an RNA-protein complex of the interacting motif and L7Ae protein has a dissociation constant Kd of approximately 0.1 nM to approximately 1 mu M between the motif and L7Ae and the interacting motif interacts with L7Ae protein.
[claim2]
2. An RNA-protein complex comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim3]
3. A translational regulatory kit comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim4]
4. A method for translational regulation of mRNA, comprising contacting the mRNA according to claim 1 with a protein specifically binding to the RNA-protein complex interacting motif nucleotide sequence.
[claim5]
5. An artificial information conversion method which converts input information of an arbitrary substrate protein to output information of an arbitrary target protein using an mRNA according to claim 1, comprising steps of preparing the mRNA of claim 1 having an open reading frame encoding the arbitrary target protein; and
contacting the mRNA with the substrate protein that specifically binds to the RNA-protein complex interacting motif nucleotide sequence.
[claim6]
6. A plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1.
[claim7]
7. An intracellular translational regulatory kit comprising a first plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1, and
a second plasmid vector comprising a nucleic acid sequence encoding a protein specifically binding to the RNA-protein complex interacting motif nucleotide sequence.
[claim8]
8. The kit according to claim 7, for regulating protein translation in a human cancer cell.
  • 発明者/出願人(英語)
  • INOUE TAN
  • SAITO HIROHIDE
  • KOBAYASHI TETSUHIRO
  • HARA TOMOAKI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • C12N015/67
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